Differential centrifugation in culture and differentiation of rat neural stem cells

doi:10.1007/s10571-007-9194-5

科研成果详情

题名	Differential centrifugation in culture and differentiation of rat neural stem cells
作者	Ye, Sheng1; Su, Zhi-Peng1; Zhang, Jing 2; Qian, Xu3; Zhuge, Qi-Chuan1; Zeng, Yan-Jun 2
发表日期	2008-06-01
发表期刊	Cellular and molecular neurobiology 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Differential centrifugation Neural stem cell Single cell suspension
摘要	(1) The study of neural stem cells (NSC) has attracted much attention in recent years because of their therapeutic potential. However, the problem in culture and differentiation of NSC was how to obtain single cell suspension that preserves the function of NSC, and remove the debris caused by mechanical dissociation. In the present study, we try to find a simple and effective way to address the problem, i.e. differential centrifugation. (2) After a gentle mechanical dissociation using Pasteur pipette, the suspension was first centrifuged at 100 g for 5 min, and then recentrifuged at 400 g for 6 min. Finally, the two deposits were resuspended and seeded into culture flask respectively. The suspension from the second deposit was allowed for further culture and differentiation. Immunofluorescence technique was used to identify neural stem cell, neuron, astrocyte, and oligodendrocyte. (3) After the second differential centrifugation, single cell suspension was obtained with 2-3 cell clusters, and the cells not only grew to form neurospheres, but also differentiated into neurons, astrocytes, and oligodendrocytes. (4) Differential centrifugation is a simple and effective way to obtain single cell suspension, which will help make large-scale production of neurodifferentiated cells more effective.
ISSN	0272-4340
卷号	28 期号:4 页码:511-7.
DOI	10.1007/s10571-007-9194-5
收录类别	PUBMED ; SCOPUS
URL	查看原文
PubMed ID	17763936
SCOPUSEID	2-s2.0-43049147095
通讯作者地址	[Zhuge, Qi-Chuan]Department of Neurosurgery,First Affiliated Hospital,Wenzhou Medical College,China
Scopus学科分类	Cellular and Molecular Neuroscience;Cell Biology
引用统计	被引频次：4[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/63147
专题	附属第一医院基础医学院（机能实验教学中心）_形态学系_组织胚胎学
通讯作者	Zhuge, Qi-Chuan
作者单位	1.Department of Neurosurgery,First Affiliated Hospital,Wenzhou Medical College,China; 2.Biomedical Engineering Center,Beijing University of Technology,China; 3.Department of Histology and Embryology,Wenzhou Medical College,China
第一作者单位	附属第一医院
通讯作者单位	附属第一医院
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Ye, Sheng,Su, Zhi-Peng,Zhang, Jing,et al. Differential centrifugation in culture and differentiation of rat neural stem cells[J]. Cellular and molecular neurobiology,2008,28(4):511-7..
APA	Ye, Sheng, Su, Zhi-Peng, Zhang, Jing, Qian, Xu, Zhuge, Qi-Chuan, & Zeng, Yan-Jun. (2008). Differential centrifugation in culture and differentiation of rat neural stem cells. Cellular and molecular neurobiology, 28(4), 511-7..
MLA	Ye, Sheng,et al."Differential centrifugation in culture and differentiation of rat neural stem cells".Cellular and molecular neurobiology 28.4(2008):511-7..