HLA-A site genotyping on single blastomeres is studied by nest-PCR-SSP method

科研成果详情

题名	HLA-A site genotyping on single blastomeres is studied by nest-PCR-SSP method
作者	Xu, Bing-Sen 1; Hu, Yong-Wu2,3,4; Huang, Xue-Feng1; Lin, Jin-Ju1; Zhou, Yin1; Ye, Bi-Lu 1; Xu, Li-Xin 1; Xu, Kan-Pu 5; Yang, Huan-Ming 3
发表日期	2006-04-01
发表期刊	Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Human leukocyte antigen genotyping Preimplantation embryo Single blastomeres
摘要	Objective: To assess the accuracy and reliability of the nest-PCR-sequence specific primer(SSP) method in HLA-A site genotyping of single blastomeres retrieved from human pre-implantation embryos. Methods: By nest PCR on HLA-A exon 2, the success rate of first-round amplification was estimated for single blastomeres. Based on the first-round amplification, the HLA-A genotype of every single blastomeres was analyzed by commercially available PCR-SSP kits. Results: The amplification of HLA-A exon 2 were performed to 120 blasotmeres retrieved from in vitro fertilization(IVF) surplus embryos donated by 10 couples. The average success rate of family 1-5 and 6-10 was 78.2%(43/55) and 93.8%(61/65), respectively. And 86.7%(104/120) in total. Eighty blastomeres were further tested by nest-PCR-SSP, among which 11 blastomeres failed to HLA-A exon 2 amplification and then failed to genotyping while the other 69 blastomeres succeed in HLA-A exon 2 amplification and succeed in genotyping. Except for 6 blastomeres that were uncertain for allele lost because of parents' homozygosity, the left 63 blastomeres had accurate HLA genotyping. Among these 63 blastomeres, 59 blastomeres had genotypes confirmed from their parents(93.6%), 3 blastomeres lost one of parents' alleles(4.8%), and only one blastomere had two more than parents' alleles(1.6%). Conclusion: The above research results indicated that based on the successful first round amplification of single blastomeres, nest-PCR-SSP strategy offers a convenient and reliable option for HLA genotyping on single blastomeres, which is a key process in pre-selecting HLA-identical sibling for allogeneic cord blood cell transplantation.
ISSN	1003-9406
卷号	23 期号:2 页码:156-60.
收录类别	PUBMED ; SCOPUS
URL	查看原文
PubMed ID	16604485
SCOPUSEID	2-s2.0-33646088954
通讯作者地址	[Huang, Xue-Feng]Department of Reproductive Medicine Centre,First Affiliated Hospital,Wenzhou Medical College,China
Scopus学科分类	Medicine (all)
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/62949
专题	附属第一医院基础医学院（机能实验教学中心）_生物科学系
通讯作者	Huang, Xue-Feng
作者单位	1.Department of Reproductive Medicine Centre,First Affiliated Hospital,Wenzhou Medical College,China; 2.Department of Biology,School of Life Sciences,Wenzhou Medical College,China; 3.Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,China; 4.Graduate School,Chinese Academy of Sciences,China; 5.Weill Medical College of Cornell University,United States
第一作者单位	附属第一医院
通讯作者单位	附属第一医院
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Xu, Bing-Sen,Hu, Yong-Wu,Huang, Xue-Feng,et al. HLA-A site genotyping on single blastomeres is studied by nest-PCR-SSP method[J]. Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics,2006,23(2):156-60..
APA	Xu, Bing-Sen., Hu, Yong-Wu., Huang, Xue-Feng., Lin, Jin-Ju., Zhou, Yin., ... & Yang, Huan-Ming. (2006). HLA-A site genotyping on single blastomeres is studied by nest-PCR-SSP method. Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics, 23(2), 156-60..
MLA	Xu, Bing-Sen,et al."HLA-A site genotyping on single blastomeres is studied by nest-PCR-SSP method".Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics 23.2(2006):156-60..

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