The application of interphase fluorescence in situ hybridization to the diagnosis of X chromosomal count abnormality in ovarian carcinoma cell

科研成果详情

题名	The application of interphase fluorescence in situ hybridization to the diagnosis of X chromosomal count abnormality in ovarian carcinoma cell
作者	Yong-Zhang Liu; Ci-Xia Shuai; Jie-Ying Dong
发表日期	2005-03-01
发表期刊	Yi chuan = Hereditas 影响因子和分区
语种	英语
原始文献类型	Article
摘要	To study the technique of fluorescence in situ hybridization (FISH) and its application value in the diagnosis of sex chromosomal count abnormality in ovarian carcinoma cell. Biotin labeled alpha satellite X chromosome DNA(pBamX7) probe was hybridized with pre-treated slides of ovarian carcinoma cell interphase nucleus in 18 cases of ovarian carcinoma specimens. The slides were treated with Avidin-FITC and Anti-avidin, amplified with an additional layer and counter-stained with PI in antifade solution. The hybridization signals as well as interphase nucleus settings were observed with WIB filters under fluorescence microscope Olympus AX-70, and the number of interphase nucleus in the ovarian carcinoma cell was counted. It was observed under the microscope that the Biotin labeled pBamX7 probe showed green hybridization signals. Cytoplasm counter-stained with PI showed reddish orange. Increased chrosome X copy number was observed in 11/18(61%) ovarian carcinoma specimens, of which the rest 7 (39%) had no increase of chrosome X copy number. Gain of X chrosome had a certain incidence in ovarian cancers, which played a role in the recurrence and development of ovarian cancers. Its significance needs further investigation.
ISSN	0253-9772
卷号	27 期号:2 页码:185-9.
收录类别	PUBMED ; SCOPUS
URL	查看原文
Pubmed记录号	15843342
Scopus记录号	2-s2.0-55249102782
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/62926
专题	基础医学院（机能实验教学中心）_生物科学系
作者单位	Department of Biology, Wenzhou Medical College, Zhejiang Wenzhou 325027, China. Lyz@wzmc.net
第一作者单位	生物科学系
第一作者的第一单位	生物科学系
推荐引用方式 GB/T 7714	Yong-Zhang Liu,Ci-Xia Shuai,Jie-Ying Dong. The application of interphase fluorescence in situ hybridization to the diagnosis of X chromosomal count abnormality in ovarian carcinoma cell[J]. Yi chuan = Hereditas,2005,27(2):185-9..
APA	Yong-Zhang Liu, Ci-Xia Shuai, & Jie-Ying Dong. (2005). The application of interphase fluorescence in situ hybridization to the diagnosis of X chromosomal count abnormality in ovarian carcinoma cell. Yi chuan = Hereditas, 27(2), 185-9..
MLA	Yong-Zhang Liu,et al."The application of interphase fluorescence in situ hybridization to the diagnosis of X chromosomal count abnormality in ovarian carcinoma cell".Yi chuan = Hereditas 27.2(2005):185-9..