Apoptosis induced by hydroquinone in bone marrow mononuclear cells in vitro

科研成果详情

题名	Apoptosis induced by hydroquinone in bone marrow mononuclear cells in vitro
作者	Yi Chen; Kang Yu; Jian-bo Wu; Zhi-jian Shen; Song-fu Jiang; Xu-dong Hu; Jun-li Zhang; Lai-xi Bi
发表日期	2004-06-01
发表期刊	Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases 影响因子和分区
语种	英语
原始文献类型	Article
摘要	Objective: To study the effect of hydroquinone on apoptosis of bone marrow mononuclear cells, and to evaluate the toxic effect of benzene on stem cells. Methods: Cell morphology was observed by HT fluorescent stain method, and DNA fragments were analyzed by agarose gel electrophoresis. Anti-Annexin V FITC plus PI staining for apoptotic and necrotic rate was examined by flow cytometer. Results: After adding different concentrations of hydroquinone to the cells for 6 h culture, the fluorescent intensity of nucleus increased, the color of nucleus became deep and inhomogeneous, and the chromatin was condensed and distributed around the neucleus. DNA ladder was detected in all samples. Cell apoptotic rate in different concentration of hydroquinone groups was significantly higher than that in blank control group (P < 0.05). With the increase of the concentration of hydroquinone, the apoptotic and necrotic rate also increased. The optimal concentration of hydroquinone was 50 micro mol/L. When it was >or= 75 micro mol/L, the necrotic rate increased significantly. Hydroquinone-induced apoptosis was associated with culture time at the concentration of 50 micro mol/L, and the peak apoptotic time was 10 h, then the apoptotic rate decreased and necrotic rate increased. Conclusion: Hydroquinone can induce apoptosis of bone marrow mononuclear cells in vitro with dose-effect and time-effect relationship.
ISSN	1001-9391
卷号	22 期号:3 页码:161-4.
收录类别	PUBMED ; SCOPUS
URL	查看原文
Pubmed记录号	15256144
Scopus记录号	2-s2.0-21644437353
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/62889
专题	附属第一医院_血液内科
作者单位	Department of Hematology, Wenzhou Medical College, Wenzhou 325000, China.
第一作者单位	血液内科; 血液内科
第一作者的第一单位	血液内科
推荐引用方式 GB/T 7714	Yi Chen,Kang Yu,Jian-bo Wu,et al. Apoptosis induced by hydroquinone in bone marrow mononuclear cells in vitro[J]. Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases,2004,22(3):161-4..
APA	Yi Chen., Kang Yu., Jian-bo Wu., Zhi-jian Shen., Song-fu Jiang., ... & Lai-xi Bi. (2004). Apoptosis induced by hydroquinone in bone marrow mononuclear cells in vitro. Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases, 22(3), 161-4..
MLA	Yi Chen,et al."Apoptosis induced by hydroquinone in bone marrow mononuclear cells in vitro".Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases 22.3(2004):161-4..

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