Detection of sperm chromosomes in Robertsonian translocation carriers by dual-color fluorescence in situ hybridization

科研成果详情

题名	Detection of sperm chromosomes in Robertsonian translocation carriers by dual-color fluorescence in situ hybridization
作者	Yongzhang Liu; Haibo Zhu
发表日期	2004-02-01
发表期刊	Zhonghua nan ke xue = National journal of andrology 影响因子和分区
语种	英语
原始文献类型	Article
摘要	Objective: To study the technique of dual-color fluorescence in situ hybridization(D-FISH) and its application value in sperm chromosomes of Robertsonian translocation carriers. Methods: The technique of dual-color fluorescence in situ hybridization was used. Biotin labelled 13q14.3 specific probe and Digoxigenin labeled 14q11.1 specific probe were used for in situ hybridization of sperm specimens in 2 Robertsonian translocation carriers. Hybridization signals for chromosomes 13 and 14 in the sperm interphase nucleus were counted. Results: Under the microscope, Biotin labeled 13q 14.3 specific probe showed 1 green hybridization signal and Digoxigenin labeled 14q 11.1 specific probe showed 1 red hybridization signal. Interphase nucleus counter-stained with DAPI showed blue. From the total of 3,000 sperm interphase nuclei, the positive rate for 1 green hybridization signal and 1 red hybridization signal was 13q/14q(39.33%), for 1 green and 2 red was 13q/14q, 14q(11.57%), for 1 green was 13q/-(9.27%), for 2 green and 1 red was 13q,13q/14q(12.87%), for 1 red was -/14q(9.87%) and for 2 green and 2 red was 13q,13q/14q, 14q(12.26%). Conclusions: D-FISH of the human sperm interphase nucleus can be applied to the determination of sperm chromosomes of Robertsonian translocation carriers and the analysis of the laws of chromosomal segregation in the meiosis. The technique can be widely used in such aspects of human reproduction as insemination under the microscope and preimplantation embryos genetic diagnosis.
ISSN	1009-3591
卷号	10 期号:2 页码:90-3.
收录类别	PUBMED ; SCOPUS
URL	查看原文
PubMed ID	15027178
SCOPUSEID	2-s2.0-2942609691
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/62878
专题	基础医学院（机能实验教学中心）_生物科学系
作者单位	Department of Biology, Wenzhou Medical College, Wenzhou, Zhejiang 325027, China. Liuyz-2@163.com
第一作者单位	基础医学院（机能实验教学中心）_生物科学系
第一作者的第一单位	基础医学院（机能实验教学中心）_生物科学系
推荐引用方式 GB/T 7714	Yongzhang Liu,Haibo Zhu. Detection of sperm chromosomes in Robertsonian translocation carriers by dual-color fluorescence in situ hybridization[J]. Zhonghua nan ke xue = National journal of andrology,2004,10(2):90-3..
APA	Yongzhang Liu, & Haibo Zhu. (2004). Detection of sperm chromosomes in Robertsonian translocation carriers by dual-color fluorescence in situ hybridization. Zhonghua nan ke xue = National journal of andrology, 10(2), 90-3..
MLA	Yongzhang Liu,et al."Detection of sperm chromosomes in Robertsonian translocation carriers by dual-color fluorescence in situ hybridization".Zhonghua nan ke xue = National journal of andrology 10.2(2004):90-3..