Immune response elicited by the recombinant protein and plasmid DNA of complex antigen ROP2-SAG1 from Toxoplasma gondii

科研成果详情

题名	Immune response elicited by the recombinant protein and plasmid DNA of complex antigen ROP2-SAG1 from Toxoplasma gondii
作者	Wen-Shu Li; Zi-Xin Xie; Qing-Xin Chen; Shao Chen; Li-Fang Zhang
发表日期	2010-10-30
发表期刊	Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases 影响因子和分区
语种	英语
原始文献类型	Article
摘要	Objective: To study the immune response elicited by the recombinant protein vaccine and DNA vaccine of the complex antigen ROP2-SAG1 from Toxoplasma gondii. Methods: Sixty female BALB/c mice were randomly divided into 4 groups (15 per group). Mice in rROP2-SAGI group were immunized subcutaneously with 2.5 microg rROP2-SAG1 protein formulated in Freund's adjuvant. Mice in control group received only adjuvant emulsified with normal saline. Mice in recombinant plasmid pcROP2-SAG1 and control plasmid pcDNA3.1 groups were each injected intramuscularly with 100 microg of pcROP2-SAG1 and pcDNA3.1, respectively. All the mice received three immunizations at 2-week intervals. Serum samples were collected at 25, 45, and 70 days after immunization for determining antibody IgG, and at 2 weeks after the last immunization IgG1 and IgG2a were detected all by ELISA. Cell counting kit-8 (CCK-8) was used to determine the splenocyte proliferation, and the supernatant of cultured splenocytes was collected for the detection of IFN-gamma by ELISA. Results: The level of IgG continued to rise in rROP2-SAG1 group after immunization, and similarly in pcROP2-SAG1 group. At 2 weeks after the last immunization, level of IgG1 (1.538 +/- 0.183) was higher than that of IgG2a (0.618 +/- 0.122) (P < 0.05) in rROP2-SAG1 group. Whereas no significant difference between IgG1 (1.107 +/- 0.137) and IgG2a (0.830 +/- 0.185) was observed in pcROP2-SAG1 group (P > 0.05). Compared with the pcROP2-SAG1 group (A450 = 0.123 +/- 0.018), more significant proliferation response of splenocytes was observed in rROP2-SAG1 group (0.348 +/- 0.042) (P < 0.05). There was no significant difference (P > 0.05) of IFN-gamma and IL-2 in the supematant of cultured splenocytes between the groups of rROP2-SAG1 and pcROP2-SAG1. Conclusion: The antibody level and splenocyte proliferation have been significantly higher in mice immunized with recombinant protein rROP2-SAG1 than those with recombinant plasmid pcROP2-SAG1.
ISSN	1000-7423
卷号	28 期号:5 页码:359-63.
收录类别	PUBMED ; SCOPUS
URL	查看原文
Pubmed记录号	21351549
Scopus记录号	2-s2.0-84879789196
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/62736
专题	基础医学院（机能实验教学中心）_病原生物学与免疫学系
作者单位	Department of Microbiology and Immunology of Wenzhou Medical College, Wenzhou 325032, China.
第一作者单位	病原生物学与免疫学系
第一作者的第一单位	病原生物学与免疫学系
推荐引用方式 GB/T 7714	Wen-Shu Li,Zi-Xin Xie,Qing-Xin Chen,et al. Immune response elicited by the recombinant protein and plasmid DNA of complex antigen ROP2-SAG1 from Toxoplasma gondii[J]. Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases,2010,28(5):359-63..
APA	Wen-Shu Li, Zi-Xin Xie, Qing-Xin Chen, Shao Chen, & Li-Fang Zhang. (2010). Immune response elicited by the recombinant protein and plasmid DNA of complex antigen ROP2-SAG1 from Toxoplasma gondii. Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases, 28(5), 359-63..
MLA	Wen-Shu Li,et al."Immune response elicited by the recombinant protein and plasmid DNA of complex antigen ROP2-SAG1 from Toxoplasma gondii".Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases 28.5(2010):359-63..

条目包含的文件		下载所有文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
ZJSB201005014.CAJ（292KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	浏览下载