Effects of NF-E2-related factor-2 promoter polymorphism on lipopolysaccharide-induced inflammatory responses in macrophages

doi:10.3760/cma.j.issn.0376-2491.2013.14.019

科研成果详情

题名	Effects of NF-E2-related factor-2 promoter polymorphism on lipopolysaccharide-induced inflammatory responses in macrophages
其他题名	Effects of NF-E2-related factor-2 promoter polymorphism on lipopolysaccharide-induced inflammatory responses in macrophages
作者	Qiao-meng Qiu; Jin-tao Zheng; Chao Nan; Yuan-yuan Zhao; Guang-ju Zhao; Guang-liang Hong; Huan Liang; Meng-Fang Li; Zhong-Qiu Lu
发表日期	2013-04-09
发表期刊	Zhonghua yi xue za zhi 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Inflammation Polymorphism, singlenucleotide Promoter regions (genetics)
其他关键词	Promoter regions(genetics) ; Polymorphism,singlenucleotide ; Inflammation
摘要	Objective: To explore the effects of NF-E2-related factor-2 (NRF2)-617C/A promoter polymorphism on NRF2 expression as well as lipopolysaccharide-induced inflammatory responses in macrophages. Methods: NRF2-617C/A promoter fragments were synthesized by chemical method and cloned into a pUC57 vector. The dul-luciferase reporter assay was employed to determine the activity of promoters. Then recombinant adenoviral vectors were constructed and transfected into macrophages. The expression of Nrf2 was examined by Western blotting and reverse transcription (RT)-PCR. The expressions of tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6) and interleukin-10 (IL-10) in macrophages after the stimulation of LPS were determined by enzyme-linked immunosorbent assay (ELISA). Results: The activity of NRF2-617C promoter-luciferase reporter (FLuc/RLuc activity ratio) was significantly higher than that of NRF2-617A group (0.584 ± 0.016 vs 0.258 ± 0.018, P < 0.05).The NRF2 protein and mRNA levels in -617C group were much higher than those of 617A group (1.123 ± 0.080 vs 0.951 ± 0.057,1.889 ± 0.031 vs 1.647 ± 0.323, both P < 0.05). After the stimulation of LPS, the NRF2 protein expression in macrophages significantly increased (0.584 ± 0.016 vs 0.258 ± 0.018, P < 0.05). Compared with -617A group, there was a significantly higher expression of NRF2 in -617C group (0.671 ± 0.033 vs 0.751 ± 0.014, P < 0.05). Additionally, the productions of IL-6 and IL-10 in -617C group were markedly lower than those in -617A group as well as IL-6/IL-10 (both P < 0.05). However, no significant difference existed in the levels of TNF-α between -617C and -617A groups (P > 0.05). Conclusions: The -617C/A promoter polymorphism of NRF2 may influence the NRF2 expression. And it appears to be associated with the LPS-induced inflammatory responses in macrophages.
其他摘要	Objective To explore the effects of NF-E2-related factor-2 (NRF2) - 617C/A promoter polymorphism on NRF2 expression as well as lipopolysaccharide-induced inflammatory responses in macrophages. Methods NRF2 -617C/A promoter fragments were synthesized by chemical method and cloned into a pUC57 vector. The dul-luciferase reporter assay was employed to determine the activity of promoters. Then recombinant adenoviral vectors were constructed and transfected into macrophages. The expression of Nrf2 was examined by Western blotting and reverse transcription (RT)-PCR. The expressions of tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6) and interleukin-10 (IL-10) in macrophages after the stimulation of LPS were determined by enzyme-linked immunosorbent assay (ELISA). Results The activity of NRF2-617C promoter-luciferase reporter(FLuc/RLuc activity ratio) was significantly higher than that of NRF2 -617A group (0. 584 ±0. 016 vs 0.258 ±0. 018, P<0. 05). The NRF2 protein and mRNA levels in -617C group were much higher than those of 617A group(1.123 ±0. 080 vs 0. 951±0.057,1. 889±0.031 vs 1.647±0.323, both P <0. 05). After the stimulation of LPS, the NRF2 protein expression in macrophages significantly increased (0. 584 ±0. 016 vs 0. 258 ±0. 018,P<0. 05). Compared with -617A group, there was a significantly higher expression of NRF2 in -617C group (0. 671 ±0. 033 vs 0.751±0.014,P<0.05). Additionally, the productions of IL-6 and IL-10 in -617C group were markedly lower than those in - 617A group as well as IL-6/IL-10 (both P<0.05). However, no significant difference existed in the levels of TNF-α between - 617C and - 617A groups(P>0.05). Conclusions The -617C/A promoter polymorphism of NRF2 may influence the NRF2 expression. And it appears to be associated with the LPS-induced inflammatory responses in macrophages.
资助项目	浙江省自然科学基金 ; 浙江省科技厅公益性技术应用研究计划 ; 浙江省温州市科技计划项目 ; 温州市高层次人才创新技术重点项目
ISSN	0376-2491
卷号	93 期号:14 页码:1114-7.
DOI	10.3760/cma.j.issn.0376-2491.2013.14.019
页数	4
收录类别	PUBMED ; SCOPUS ; CSCD ; 万方 ; ISTIC ; PKU ; 北大核心
学科领域	医药、卫生 ; 临床医学
URL	查看原文
CSCD记录号	CSCD:4794114
Pubmed记录号	23902849
Scopus记录号	2-s2.0-84876787025
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/62627
专题	第一临床医学院（信息与工程学院）、附属第一医院_急诊医学
作者单位	Department of Emergency Medicine, Wenzhou Medical College, Wenzhou, China.
第一作者单位	温州医科大学
第一作者的第一单位	温州医科大学
推荐引用方式 GB/T 7714	Qiao-meng Qiu,Jin-tao Zheng,Chao Nan,et al. Effects of NF-E2-related factor-2 promoter polymorphism on lipopolysaccharide-induced inflammatory responses in macrophages[J]. Zhonghua yi xue za zhi,2013,93(14):1114-7..
APA	Qiao-meng Qiu., Jin-tao Zheng., Chao Nan., Yuan-yuan Zhao., Guang-ju Zhao., ... & Zhong-Qiu Lu. (2013). Effects of NF-E2-related factor-2 promoter polymorphism on lipopolysaccharide-induced inflammatory responses in macrophages. Zhonghua yi xue za zhi, 93(14), 1114-7..
MLA	Qiao-meng Qiu,et al."Effects of NF-E2-related factor-2 promoter polymorphism on lipopolysaccharide-induced inflammatory responses in macrophages".Zhonghua yi xue za zhi 93.14(2013):1114-7..