Mitochondrial Uncoupling Protein 2 in human cumulus cells is associated with regulating autophagy and apoptosis, maintaining gap junction integrity and progesterone synthesis

doi:10.1016/j.mce.2017.01.020

科研成果详情

题名	Mitochondrial Uncoupling Protein 2 in human cumulus cells is associated with regulating autophagy and apoptosis, maintaining gap junction integrity and progesterone synthesis
作者	Ge, Hongshan1,2; Zhang, Fan 2; Duan, Ping2; Zhu, Nan2; Zhang, Jiayan2; Ye, Feijun 3; Shan, Dan2; Chen, Hua2; Lu, XiaoSheng2; Zhu, ChunFang2; Ge, Renshan2; Lin, Zhenkun2
发表日期	2017-03-05
发表期刊	MOLECULAR AND CELLULAR ENDOCRINOLOGY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Uncoupling Protein 2 Cumulus cells Autophagy Apoptosis Gap junction Progesterone synthesis
其他关键词	GRANULOSA-CELLS ; INTERCELLULAR COMMUNICATION ; DEVELOPMENTAL COMPETENCE ; LIFE-SPAN ; UCP2 ; MOUSE ; EXPRESSION ; CONNEXIN43 ; STEROIDOGENESIS ; MATURATION
摘要	To explore the roles of mitochondrial Uncoupling Protein 2 (UCP2) in cumulus cells (CCs), human CCs were cultured in vitro, and the UCP2 was inhibited by treatment with Genipin, a special UCP inhibitor, or by RNA interference targeting UCP2. No significant differences in adenosine triphosphate levels and the ratio of ADP/ATP were observed after UCP2 inhibition. UCP2 inhibition caused a significant increase in cellular oxidative damage, which was reflected in alterations to several key parameters, including reactive oxygen species (ROS) and lipid peroxidation levels and the ratio of reduced GSH to GSSG. UCP2 blocking resulted in an obvious increase in active Caspase-3, accompanied by the decline of proactive Caspase-3 and a significant increase in the LC3-II/LC3-I ratio, suggesting that UCP2 inhibition triggered cellular apoptosis and autophagy. The mRNA and protein expression of connexin 43 (Cx43), a gap junction channel protein, were significantly reduced after treatment with Genipin or siRNA. The progesterone level in the culture medium was also significantly decreased after UCP2 inhibition. Our data indicated that UCP2 plays highly important roles in mediating ROS production and regulating apoptosis and autophagy, as well as maintaining gap junction integrity and progesterone synthesis, which suggests that UCP2 is involved in the regulation of follicle development and early embryo implantation and implies that it might serve as a potential biomarker for oocyte quality and competency. (C) 2017 Elsevier B.V. All rights reserved.
资助项目	Non-profit research project of Science and Technology Department of Zhejiang province [2014C33162, 2014C37003]; Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81370674]; project of Department of Education of Zhejiang Province [Y201327892]; project of science and technology of Zhoushan [2012C13030]
出版者	ELSEVIER IRELAND LTD
出版地	CLARE
ISSN	0303-7207
EISSN	1872-8057
卷号	443 期号:C 页码:128-137
DOI	10.1016/j.mce.2017.01.020
页数	10
WOS类目	Cell Biology ; Endocrinology & Metabolism
WOS研究方向	Cell Biology ; Endocrinology & Metabolism
WOS记录号	WOS:000397072100014
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	28089824
SCOPUSEID	2-s2.0-85009807712
通讯作者地址	[Ge, Hongshan]Taizhou People's Hospital,Taizhou,China
Scopus学科分类	Biochemistry;Molecular Biology;Endocrinology
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/6113
专题	第二临床医学院、附属第二医院、育英儿童医院附属第二医院其他_附属舟山医院（舟山医院）
通讯作者	Ge, Hongshan
作者单位	1.Center for Reproductive Medicine,Department of Obstetrics and Gynaecology,Taizhou People's Hospital,The Fifth Hospital Affiliated Nantong University,Taizhou,225300,China; 2.The Second Affiliated Hospital and YuYing Children's Hospital of Wenzhou Medical University,Wenzhou,325000,China; 3.Maternal and Child Health Hospital,Zhoushan Hospital Affiliated Wenzhou Medical University,Zhejiang Province,316100,China
第一作者单位	第二临床医学院，附属第二医院、育英儿童医院; 附属第二医院
推荐引用方式 GB/T 7714	Ge, Hongshan,Zhang, Fan,Duan, Ping,et al. Mitochondrial Uncoupling Protein 2 in human cumulus cells is associated with regulating autophagy and apoptosis, maintaining gap junction integrity and progesterone synthesis[J]. MOLECULAR AND CELLULAR ENDOCRINOLOGY,2017,443(C):128-137.
APA	Ge, Hongshan., Zhang, Fan., Duan, Ping., Zhu, Nan., Zhang, Jiayan., ... & Lin, Zhenkun. (2017). Mitochondrial Uncoupling Protein 2 in human cumulus cells is associated with regulating autophagy and apoptosis, maintaining gap junction integrity and progesterone synthesis. MOLECULAR AND CELLULAR ENDOCRINOLOGY, 443(C), 128-137.
MLA	Ge, Hongshan,et al."Mitochondrial Uncoupling Protein 2 in human cumulus cells is associated with regulating autophagy and apoptosis, maintaining gap junction integrity and progesterone synthesis".MOLECULAR AND CELLULAR ENDOCRINOLOGY 443.C(2017):128-137.