4-Hydroxyphenylpyruvate Dioxygenase-Like Protein Promotes Pancreatic Cancer Cell Progression and Is Associated With Glutamine-Mediated Redox Balance

doi:10.3389/fonc.2020.617190

科研成果详情

题名	4-Hydroxyphenylpyruvate Dioxygenase-Like Protein Promotes Pancreatic Cancer Cell Progression and Is Associated With Glutamine-Mediated Redox Balance
作者	Ye, Xianglai 1,2,3; Wei, Xiujuan1,2,3; Liao, Jing1,2,3; Chen, Peipei 1,2,3; Li, Xueyun1,2,3; Chen, Yulong1,2,3; Yang, Yue 1,2,3; Zhao, Qiongya 4; Sun, Hongwei5; Pan, Liming 6; Chen, Guorong7; He, Xujun 8; Lyu, Jianxin 1,2,3,4,8; Fang, Hezhi1,2,3
发表日期	2021-01-18
发表期刊	FRONTIERS IN ONCOLOGY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	mitochondria pancreatic ductal adenocarcinoma metabolic reprogramming glutamine redox balance
其他关键词	MITOCHONDRIA ; METABOLISM ; TUMORS
摘要	Tumor cells develop a series of metabolic reprogramming mechanisms to meet the metabolic needs for tumor progression. As metabolic hubs in cells, mitochondria play a significant role in this process, including energy production, biosynthesis, and redox hemostasis. In this study, we show that 4-hydroxyphenylpyruvate dioxygenase-like protein (HPDL), a previously uncharacterized protein, is positively associated with the development of pancreatic ductal adenocarcinoma (PDAC) and disease prognosis. We found that overexpression of HPDL in PDAC cells promotes tumorigenesis in vitro, whereas knockdown of HPDL inhibits cell proliferation and colony formation. Mechanistically, we found that HPDL is a mitochondrial intermembrane space localized protein that positively regulates mitochondrial bioenergetic processes and adenosine triphosphate (ATP) generation in a glutamine dependent manner. Our results further reveal that HPDL protects cells from oxidative stress by reprogramming the metabolic profile of PDAC cells toward glutamine metabolism. In short, we conclude that HPDL promotes PDAC likely through its effects on glutamine metabolism and redox balance.
资助项目	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31670784, 82072366]; Natural Science Foundation of Zhejiang ProvinceNatural Science Foundation of Zhejiang Province [Y20H160175]; Research Fund of National Health Commission; Major Medical Research Program of Zhejiang Province [WKJ-ZJ-1929]; Technology and Science Project of Yuhuan City [201741]
出版者	FRONTIERS MEDIA SA
出版地	LAUSANNE
ISSN	2234-943X
卷号	10 页码:617190
DOI	10.3389/fonc.2020.617190
页数	14
WOS类目	Oncology
WOS研究方向	Oncology
WOS记录号	WOS:000613346900001
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	33537239
PMC记录号	PMC7848781
SCOPUSEID	2-s2.0-85100590098
通讯作者地址	[Lyu, Jianxin]Key Laboratory of Laboratory Medicine,Ministry of Education,Wenzhou Medical University,Wenzhou,China ; [Fang, Hezhi]Key Laboratory of Laboratory Medicine,Ministry of Education,Wenzhou Medical University,Wenzhou,China ; [Lyu, Jianxin]Zhejiang Provincial Key Laboratory of Medical Genetics,Department of Cell Biology and Medical Genetics,Wenzhou Medical University,Wenzhou,China ; [Fang, Hezhi]Zhejiang Provincial Key Laboratory of Medical Genetics,Department of Cell Biology and Medical Genetics,Wenzhou Medical University,Wenzhou,China ; [Lyu, Jianxin]College of Laboratory Medicine and Life Sciences,Wenzhou Medical University,Wenzhou,China ; [Fang, Hezhi]College of Laboratory Medicine and Life Sciences,Wenzhou Medical University,Wenzhou,China ; [Lyu, Jianxin]College of Laboratory Medicine,Hangzhou Medical College,Hangzhou,China ; [Lyu, Jianxin]Department of Laboratory Medicine,Zhejiang Provincial People’s Hospital,Affiliated People’s Hospital of Hangzhou Medical College,Hangzhou,China
Scopus学科分类	Oncology;Cancer Research
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/6083
专题	基础医学院（机能实验教学中心）_生物科学系_细胞生物学检验医学院（生命科学学院、生物学实验教学中心）附属第一医院
通讯作者	Lyu, Jianxin; Fang, Hezhi
作者单位	1.Key Laboratory of Laboratory Medicine,Ministry of Education,Wenzhou Medical University,Wenzhou,China; 2.Zhejiang Provincial Key Laboratory of Medical Genetics,Department of Cell Biology and Medical Genetics,Wenzhou Medical University,Wenzhou,China; 3.College of Laboratory Medicine and Life Sciences,Wenzhou Medical University,Wenzhou,China; 4.College of Laboratory Medicine,Hangzhou Medical College,Hangzhou,China; 5.Department of Surgery,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China; 6.Department of Pathology,The People’s Hospital of Yuhuan,The Yuhuan Branch of the First Affiliated Hospital of Wenzhou Medical University,Taizhou,China; 7.Department of Pathology,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China; 8.Department of Laboratory Medicine,Zhejiang Provincial People’s Hospital,Affiliated People’s Hospital of Hangzhou Medical College,Hangzhou,China
第一作者单位	温州医科大学; 检验医学院（生命科学学院、生物学实验教学中心）
通讯作者单位	温州医科大学; 检验医学院（生命科学学院、生物学实验教学中心）
第一作者的第一单位	温州医科大学
推荐引用方式 GB/T 7714	Ye, Xianglai,Wei, Xiujuan,Liao, Jing,et al. 4-Hydroxyphenylpyruvate Dioxygenase-Like Protein Promotes Pancreatic Cancer Cell Progression and Is Associated With Glutamine-Mediated Redox Balance[J]. FRONTIERS IN ONCOLOGY,2021,10:617190.
APA	Ye, Xianglai., Wei, Xiujuan., Liao, Jing., Chen, Peipei., Li, Xueyun., ... & Fang, Hezhi. (2021). 4-Hydroxyphenylpyruvate Dioxygenase-Like Protein Promotes Pancreatic Cancer Cell Progression and Is Associated With Glutamine-Mediated Redox Balance. FRONTIERS IN ONCOLOGY, 10, 617190.
MLA	Ye, Xianglai,et al."4-Hydroxyphenylpyruvate Dioxygenase-Like Protein Promotes Pancreatic Cancer Cell Progression and Is Associated With Glutamine-Mediated Redox Balance".FRONTIERS IN ONCOLOGY 10(2021):617190.