MiR-301a promotes embryonic stem cell differentiation to cardiomyocytes

doi:10.4252/wjsc.v11.i12.1130

科研成果详情

题名	MiR-301a promotes embryonic stem cell differentiation to cardiomyocytes
作者	Zhen, Li-Xiao 1,2; Gu, Yu-Ying 1,2; Zhao, Qian 1,2; Zhu, Hui-Fang 1; Lv, Jin-Hui 1,2; Li, Shu-Jun 1,2; Xu, Zhen 3; Li, Li 1,2; Yu, Zuo-Ren 1,2
发表日期	2019-12-26
发表期刊	WORLD JOURNAL OF STEM CELLS 影响因子和分区
语种	英语
原始文献类型	Article
关键词	miR-301a Mouse embryonic stem cells Differentiation Cardiomyocytes
其他关键词	PROLIFERATION ; EXPRESSION ; PROGENITORS
摘要	BACKGROUND Cardiovascular disease is the leading cause of death worldwide. Tissue repair after pathological injury in the heart remains a major challenge due to the limited regenerative ability of cardiomyocytes in adults. Stem cell-derived cardiomyocytes provide a promising source for the cell transplantation-based treatment of injured hearts. AIM To explore the function and mechanisms of miR-301a in regulating cardiomyocyte differentiation of mouse embryonic stem (mES) cells, and provide experimental evidence for applying miR-301a to the cardiomyocyte differentiation induction from stem cells. METHODS mES cells with or without overexpression of miR-301a were applied for all functional assays. The hanging drop technique was applied to form embryoid bodies from mES cells. Cardiac markers including GATA-4, TBX5, MEF2C, and alpha-actinin were used to determine cardiomyocyte differentiation from mES cells. RESULTS High expression of miR-301a was detected in the heart from late embryonic to neonatal mice. Overexpression of miR-301a in mES cells significantly induced the expression of cardiac transcription factors, thereby promoting cardiomyocyte differentiation and beating cardiomyocyte clone formation. PTEN is a target gene of miR-301a in cardiomyocytes. PTEN-regulated PI3K-AKT-mTOR-Stat3 signaling showed involvement in regulating miR-301a-promoted cardiomyocyte differentiation from mES cells. CONCLUSION MiR-301a is capable of promoting embryonic stem cell differentiation to cardiomyocytes.
资助项目	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81800243]; Science and Technology Commission of Shanghai MunicipalityScience & Technology Commission of Shanghai Municipality (STCSM) [18411965900]; Fundamental Research Funds for the Central UniversitiesFundamental Research Funds for the Central Universities [22120180125]
出版者	BAISHIDENG PUBLISHING GROUP INC
出版地	PLEASANTON
ISSN	1948-0210
卷号	11 期号:12 页码:1130-1141
DOI	10.4252/wjsc.v11.i12.1130
页数	12
WOS类目	Cell & Tissue Engineering ; Cell Biology
WOS研究方向	Cell Biology
WOS记录号	WOS:000512755700007
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
Pubmed记录号	31875873
PMC记录号	PMC6904867
Scopus记录号	2-s2.0-85076777353
通讯作者地址	[Yu, Zuo-Ren]Key Laboratory of Arrhythmias of the Ministry of Education of China,Tongji University School of Medicine,Shanghai,200120,China
scopus学科分类	Histology;Molecular Biology;Genetics;Genetics (clinical);Cell Biology
引用统计	被引频次：4[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/5973
专题	检验医学院（生命科学学院、生物学实验教学中心）_病原生物学与免疫学系基础医学院（机能实验教学中心）_病原生物学与免疫学系
通讯作者	Yu, Zuo-Ren
作者单位	1.Key Laboratory of Arrhythmias of the Ministry of Education of China,Tongji University School of Medicine,Shanghai,200120,China; 2.Research Center for Translational Medicine,Shanghai East Hospital,Tongji University School of Medicine,Shanghai,200120,China; 3.Department of Microbiology and Immunology,Wenzhou Medical College,Wenzhou, Zhejiang Province,325000,China
推荐引用方式 GB/T 7714	Zhen, Li-Xiao,Gu, Yu-Ying,Zhao, Qian,et al. MiR-301a promotes embryonic stem cell differentiation to cardiomyocytes[J]. WORLD JOURNAL OF STEM CELLS,2019,11(12):1130-1141.
APA	Zhen, Li-Xiao., Gu, Yu-Ying., Zhao, Qian., Zhu, Hui-Fang., Lv, Jin-Hui., ... & Yu, Zuo-Ren. (2019). MiR-301a promotes embryonic stem cell differentiation to cardiomyocytes. WORLD JOURNAL OF STEM CELLS, 11(12), 1130-1141.
MLA	Zhen, Li-Xiao,et al."MiR-301a promotes embryonic stem cell differentiation to cardiomyocytes".WORLD JOURNAL OF STEM CELLS 11.12(2019):1130-1141.

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