SOX4-mediated repression of specific tRNAs inhibits proliferation of human glioblastoma cells

doi:10.1073/pnas.1920200117

科研成果详情

题名	SOX4-mediated repression of specific tRNAs inhibits proliferation of human glioblastoma cells
作者	Yang, Jianjing1,2,3; Smith, Derek K.3,4; Ni, Haoqi1,2,3; Wu, Ke 1,2; Huang, Dongdong1,2; Pan, Sishi 1,2,3; Sathe, Adwait A.5; Tang, Yu 3,4; Liu, Meng-Lu 3,4; Xing, Chao 5,6,7; Zhang, Chun-Li 3,4; Zhuge, Qichuan1,2
发表日期	2020-03-17
发表期刊	PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 影响因子和分区
语种	英语
原始文献类型	Article
关键词	tRNAi(Met) tRNA expression SOX4 glioblastoma mRNA translation
其他关键词	POLYMERASE-III TRANSCRIPTION ; INITIATING CELLS ; EXPRESSION ; GENES ; SRY ; DEPHOSPHORYLATION ; TARGETS ; SOX4 ; MAF1 ; P53
摘要	Transfer RNAs (tRNAs) are products of RNA polymerase III (Pol III) and essential for mRNA translation and ultimately cell growth and proliferation. Whether and how individual tRNA genes are specifically regulated is not clear. Here, we report that SOX4, a well-known Pol II-dependent transcription factor that is critical for neurogenesis and reprogramming of somatic cells, also directly controls, unexpectedly, the expression of a subset of tRNA genes and therefore protein synthesis and proliferation of human glioblastoma cells. Genome-wide location analysis through chromatin immunoprecipitation-sequencing uncovers specific targeting of SOX4 to a subset of tRNA genes, including those for tRNAiMet. Mechanistically, sequence-specific SOX4-binding impedes the recruitment of TATA box binding protein and Pol III to tRNA genes and thereby represses their expression. CRISPR/Cas9-mediated down-regulation of tRNAiMet greatly inhibits growth and proliferation of human glioblastoma cells. Conversely, ectopic tRNAiMet partially rescues SOX4-mediated repression of cell proliferation. Together, these results uncover a regulatory mode of individual tRNA genes to control cell behavior. Such regulation may coordinate codon usage and translation efficiency to meet the demands of diverse tissues and cell types, including cancer cells.
资助项目	Welch FoundationThe Welch Foundation [I-1724]; Decherd Foundation; Pape Adams Foundation; Texas Institute for Brain Injury and Repair; NIHUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USA [NS099073, NS092616, NS088095, NS093502]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81771262]; Zhejiang Health Science and Technology Project [2016RCA022]; Zhejiang Key Research and Development Project [2017C03027]; Kent Waldrep Foundation Center for Basic Research on Nerve Growth and Regeneration
出版者	NATL ACAD SCIENCES
出版地	WASHINGTON
ISSN	0027-8424
EISSN	1091-6490
卷号	117 期号:11 页码:5782-5790
DOI	10.1073/pnas.1920200117
页数	9
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
WOS记录号	WOS:000520011000036
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
Pubmed记录号	32123087
PMC记录号	PMC7084149
Scopus记录号	2-s2.0-85081891532
自科自定义期刊分类	T3(A)类
通讯作者地址	[Zhang, Chun-Li]Department of Molecular Biology,University of Texas Southwestern Medical Center,Dallas,75390,United States
scopus学科分类	Multidisciplinary
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/5849
专题	附属第一医院_神经外科第一临床医学院（信息与工程学院）、附属第一医院_老化与神经疾病重点实验室
通讯作者	Zhang, Chun-Li
作者单位	1.Department of Neurosurgery,First Affiliated Hospital of Wenzhou Medical University,Wenzhou, Zhejiang,325000,China; 2.Zhejiang Provincial Key Laboratory of Aging and Neurological Disorder Research,First Affiliated Hospital of Wenzhou Medical University,Wenzhou, Zhejiang,325000,China; 3.Department of Molecular Biology,University of Texas Southwestern Medical Center,Dallas,75390,United States; 4.Hamon Center for Regenerative Science and Medicine,University of Texas Southwestern Medical Center,Dallas,75390,United States; 5.McDermott Center of Human Growth and Development,University of Texas Southwestern Medical Center,Dallas,75390,United States; 6.Department of Bioinformatics,University of Texas Southwestern Medical Center,Dallas,75390,United States; 7.Department of Population and Data Sciences,University of Texas Southwestern Medical Center,Dallas,75390,United States
第一作者单位	附属第一医院
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Yang, Jianjing,Smith, Derek K.,Ni, Haoqi,et al. SOX4-mediated repression of specific tRNAs inhibits proliferation of human glioblastoma cells[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2020,117(11):5782-5790.
APA	Yang, Jianjing., Smith, Derek K.., Ni, Haoqi., Wu, Ke., Huang, Dongdong., ... & Zhuge, Qichuan. (2020). SOX4-mediated repression of specific tRNAs inhibits proliferation of human glioblastoma cells. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 117(11), 5782-5790.
MLA	Yang, Jianjing,et al."SOX4-mediated repression of specific tRNAs inhibits proliferation of human glioblastoma cells".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 117.11(2020):5782-5790.