Effect and mechanism of andrographolide on the recovery of Pseudomonas aeruginosa susceptibility to several antibiotics

doi:10.1177/147323000803600123

科研成果详情

题名	Effect and mechanism of andrographolide on the recovery of Pseudomonas aeruginosa susceptibility to several antibiotics
作者	Wu C.M.1; Cao J.L.1; Zheng M.H.2; Ou Y.1; Zhang L.2; Zhu X.Q.1; Song, Jiansing X.3
发表日期	2008-01
发表期刊	JOURNAL OF INTERNATIONAL MEDICAL RESEARCH 影响因子和分区
语种	英语
原始文献类型	Article
关键词	andrographolide antibiotics Pseudomonas aeruginosa microbes drug resistance efflux pump
其他关键词	OPRM EFFLUX-PUMP ; QUORUM-SENSING AUTOINDUCER ; GRAM-NEGATIVE BACTERIA ; MEXAB-OPRM ; RESISTANCE ; EXPRESSION ; REGULATOR ; GENE
摘要	Effectiveness and mechanism of action of andrographolide on the recovery of Pseudomonas aeruginosa susceptibility to antibiotics was investigated. In the presence of andrographolide, the Mueller-Hinton broth dilution method measured minimal inhibitory concentrations (MIC) of ceftazidine, cefpirome, chloramphenicol, L-ofloxacin, kanamycin, imipenem and meropenem. Real-time fluorescence quantitative polymerase chain reaction was used to determine mexB mRNA expressions in P. aeruginosa PAO1 strain and MexAB-OprM overexpressing strain. Relative mexB mRNA expression was detected in both strains incubated for 3 and 9 h. When andrographolide-treated groups were compared with controls, the MIC of ceftazidine, cefpirome, L-Ofloxacin and meropenem for both strains decreased, and the relative mexB mRNA expression was significantly lower, although between andrographolide groups there were no significant differences. Compared with the inactivated quorum-sensing system, relative amounts of mexB mRNA in the PAO1 strain and MexAB-OprM overexpressing strain in the activated quorum-sensing system increased 10- and 30-fold, respectively. Andrographolide recovered P. aeruginosa susceptibility to antibiotics and reduced the MexAB-OprM efflux pump expression level.
出版者	SAGE PUBLICATIONS LTD
出版地	LONDON
ISSN	0300-0605
EISSN	1473-2300
卷号	36 期号:1 页码:178-186
DOI	10.1177/147323000803600123
页数	9
WOS类目	Medicine, Research & Experimental ; Pharmacology & Pharmacy
WOS研究方向	Research & Experimental Medicine ; Pharmacology & Pharmacy
WOS记录号	WOS:000253171700023
收录类别	SCIE ; SCOPUS
URL	查看原文
PubMed ID	18304418
SCOPUSEID	2-s2.0-39149119645
通讯作者地址	[Song J.]Department of Infectious Diseases,Tongji Hospital of Tongji Medical College,Huazhong University of Science and Technology,China
Scopus学科分类	Biochemistry;Cell Biology;Biochemistry (medical)
引用统计	被引频次：19[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/5781
专题	第一临床医学院（信息与工程学院）、附属第一医院_内科学_感染内科附属第一医院
作者单位	1.Department of Liver Diseases,The Traditional Chinese Medical Hospital of Wenzhou,China; 2.Department of Infection and Liver Diseases,Wenzhou Medical College,China; 3.Department of Infectious Diseases,Tongji Hospital of Tongji Medical College,Huazhong University of Science and Technology,China
推荐引用方式 GB/T 7714	Wu C.M.,Cao J.L.,Zheng M.H.,et al. Effect and mechanism of andrographolide on the recovery of Pseudomonas aeruginosa susceptibility to several antibiotics[J]. JOURNAL OF INTERNATIONAL MEDICAL RESEARCH,2008,36(1):178-186.
APA	Wu C.M.., Cao J.L.., Zheng M.H.., Ou Y.., Zhang L.., ... & Song, Jiansing X.. (2008). Effect and mechanism of andrographolide on the recovery of Pseudomonas aeruginosa susceptibility to several antibiotics. JOURNAL OF INTERNATIONAL MEDICAL RESEARCH, 36(1), 178-186.
MLA	Wu C.M.,et al."Effect and mechanism of andrographolide on the recovery of Pseudomonas aeruginosa susceptibility to several antibiotics".JOURNAL OF INTERNATIONAL MEDICAL RESEARCH 36.1(2008):178-186.