Toxicity Assessment of Combined Fluoroquinolone and Tetracycline Exposure in Zebrafish (Danio rerio)

doi:10.1002/tox.22087

科研成果详情

题名	Toxicity Assessment of Combined Fluoroquinolone and Tetracycline Exposure in Zebrafish (Danio rerio)
作者	Zhang, Yuna 1; Wang, Xuedong 1; Yin, Xiaohan 1; Shi, Mengru2; Dahlgren, Randy Alan 3; Wang, Huili2
发表日期	2016-06
发表期刊	ENVIRONMENTAL TOXICOLOGY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	combined exposure behavioral toxicity malformation rate histopathologic analysis gene transcriptional level
其他关键词	SOLID-PHASE EXTRACTION ; WASTE-WATER ; IN-VIVO ; MESSENGER-RNA ; ANTIBIOTICS ; DRUG ; ASSAYS ; MODEL ; SOIL ; PHARMACEUTICALS
摘要	Fluoroquinolones (FQs) and tetracyclines (TCs), the two beta-diketone antibiotics (DKAs), are two frequently detected pollutants in the environment; however, little data are available on their combined toxicity to zebrafish (Danio rerio). This study reports that toxicologic effects of combined DKA (FQs-TCs) exposure on zebrafish were comparable with or slightly less than those of TCs alone, showing that TCs played a major toxicologic role in the mixtures. The effects of FQs, TCs, and DKAs on malformation rates of zebrafish were dose dependent, with EC50 values of 481.3, 16.4, and 135.1 mg/L, respectively. According to the combined effects of DKAs on zebrafish hatching, mortality, and malformation rates, the interaction between FQs and TCs was shown to be antagonistic based on three assessment methods: Toxic Unit, Additional Index, and Mixture Toxic Index. The 1.56 mg/LTC and 9.38 mg/L DKA treatments resulted in higher zebrafish basal swimming rate compared with the control group at 120 hours postfertilization (hpf). in both light and light-to-dark photoperiod experiments. Under conditions of no obvious abnormality in cardiac development, the heart beats were decreased significantly because of DKA exposure, such as decreasing by similar to 20% at 150 mg/L DKAs. Transmission electron microscopy observation of myocytes from DKA-exposed hearts displayed prominent interruptions and myofibrillar disorganization of the normal parallel alignment of thick and thin filaments, and partial edematous and dissolved membranes of cell nuclear tissues. At 90 mg/L DKAs, the transcriptional levels of the acta1a, myl7, and gle1b genes, related to heart development and skeletal muscle formation, were significantly changed. This is consistent with the swimming behavior and histopathologic results obtained by transmission electron microscopy. In summary, the toxicity of the combined DKAs to zebrafish was comparable with or less than that of TCs alone and had the ability to impair individual behaviors that are of great importance in the assessment of their ecologic fitness. (C) 2014 Wiley Periodicals, Inc.
资助项目	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31270548, 21377100]; Zhejiang Provincial Environmental Protection Department [2012B014]; Science and Technology Project of Wenzhou City [20100299]
出版者	WILEY
出版地	HOBOKEN
ISSN	1520-4081
EISSN	1522-7278
卷号	31 期号:6 页码:736-750
DOI	10.1002/tox.22087
页数	64
WOS类目	Environmental Sciences ; Toxicology ; Water Resources
WOS研究方向	Environmental Sciences & Ecology ; Toxicology ; Water Resources
WOS记录号	WOS:000378297500010
收录类别	SCIE ; PUBMED ; EI ; SCOPUS
EI入藏号	20161902356430
EI主题词	Toxicity
URL	查看原文
PubMed ID	25504783
SCOPUSEID	2-s2.0-84964727245
通讯作者地址	[Wang, Huili]School of Life Sciences,Wenzhou Medical University,University-Town,Wenzhou, Zhejiang,325035,China
Scopus学科分类	Toxicology;Management, Monitoring, Policy and Law;Health, Toxicology and Mutagenesis
引用统计	被引频次：43[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/5701
专题	公共卫生学院_水环境应用技术研究所检验医学院（生命科学学院、生物学实验教学中心）
通讯作者	Wang, Huili
作者单位	1.Wenzhou Applied Technology and Environmental Research Institute,Wenzhou Medical University,University-Town,Wenzhou, Zhejiang,325035,China; 2.School of Life Sciences,Wenzhou Medical University,University-Town,Wenzhou, Zhejiang,325035,China; 3.Department of Land,Air and Water Resources,University of California,Davis,95616,United States
第一作者单位	温州医科大学
通讯作者单位	检验医学院（生命科学学院、生物学实验教学中心）
第一作者的第一单位	温州医科大学
推荐引用方式 GB/T 7714	Zhang, Yuna,Wang, Xuedong,Yin, Xiaohan,et al. Toxicity Assessment of Combined Fluoroquinolone and Tetracycline Exposure in Zebrafish (Danio rerio)[J]. ENVIRONMENTAL TOXICOLOGY,2016,31(6):736-750.
APA	Zhang, Yuna, Wang, Xuedong, Yin, Xiaohan, Shi, Mengru, Dahlgren, Randy Alan, & Wang, Huili. (2016). Toxicity Assessment of Combined Fluoroquinolone and Tetracycline Exposure in Zebrafish (Danio rerio). ENVIRONMENTAL TOXICOLOGY, 31(6), 736-750.
MLA	Zhang, Yuna,et al."Toxicity Assessment of Combined Fluoroquinolone and Tetracycline Exposure in Zebrafish (Danio rerio)".ENVIRONMENTAL TOXICOLOGY 31.6(2016):736-750.