Two novel squamous cell carcinoma antigen-derived HLA-A*0201-binding peptides induce in vitro and in vivo CD8(+) cytotoxic T lymphocyte responses

doi:10.3892/ijo.2013.1834

科研成果详情

题名	*Two novel squamous cell carcinoma antigen-derived HLA-A0201-binding peptides induce in vitro and in vivo CD8(+) cytotoxic T lymphocyte responses**
作者	Duan, Zhi-Liang1,2; Wang, Zhi-Bin 1; Guo, Jiang-Long 1; Liu, Wen-Quan3; Hu, Jun 4; Li, Jing 1; Wang, Si-Na 1; Li, Qiang 5; Wen, Jin-Sheng1
发表日期	2013-04
发表期刊	INTERNATIONAL JOURNAL OF ONCOLOGY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	squamous cell carcinoma antigen HLA-A*0201 epitope cytotoxic T lymphocytes
其他关键词	TUMOR-ANTIGEN ; PROGNOSTIC-SIGNIFICANCE ; HEPATITIS-C ; SCC ANTIGEN ; SERPIN ; CTL ; IDENTIFICATION ; VIRUS ; EPITOPE ; CANCER
摘要	Squamous cell carcinoma antigen (SCCA) is over-expressed in many squamous cell cancers and SCCA-derived peptide-specific CD8(+) cytotoxic T lymphocytes can display cytotoxicity against tumor cells. In the present study, we screened the SCCA amino acid sequence for potential HLA-A0201-binding CD8(+) T-cell epitopes using two predictive computational algorithms. Seven epitope candidates were selected of which SCCA(246-254)(LLPNEIDGL), SCCA(223-231)(SLEDVQAKV), SCCA(328-336)(VLHKAFVEV) and SCCA(324-332)(VLSGVLHKA) significantly stabilized HLA-A0201 molecules on T2 cells. Both SCCA(328-336) and SCCA(324-332) induced CD8(+) IFN-gamma(+) T-cell responses in HLA-A0201-positive peripheral blood mononuclear cells as assessed by intracellular cytokine staining. Consistent with this, immunization with either SCCA(328-336) or SCCA(324-332) effectively elicited CD8(+) IFN-gamma(+) T cells in HLA-A0201 transgenic mice as visualized by IFN-gamma ELISPOT assay and intracellular cytokine staining. Furthermore, CD8(+) T cells induced in vitro or in vivo by SCCA(328-336) or SCCA(324-332) demonstrated in vitro cytotoxicity against peptide-pulsed T2 cells and splenocytes, respectively. These novel SCCA-derived CD8(+) T-cell epitopes described, herein, may be potentially important components for diagnostic reagents and immunotherapeutic vaccines for the treatment of squamous cell carcinomas.
资助项目	National Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [30800050, 31070143]; Natural Science Foundation of Zhejiang provinceNatural Science Foundation of Zhejiang Province [Y2090744]; Planned Science and Technology Project of Wenzhou [H20100066, Y20090338]
出版者	SPANDIDOS PUBL LTD
出版地	ATHENS
ISSN	1019-6439
EISSN	1791-2423
卷号	42 期号:4 页码:1482-1492
DOI	10.3892/ijo.2013.1834
页数	11
WOS类目	Oncology
WOS研究方向	Oncology
WOS记录号	WOS:000316511200042
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	23426430
SCOPUSEID	2-s2.0-84874594116
通讯作者地址	[Wen, Jin-Sheng]Department of Microbiology and Immunology,Wenzhou Medical College,China
Scopus学科分类	Oncology;Cancer Research
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/5667
专题	检验医学院（生命科学学院、生物学实验教学中心）_病原生物学与免疫学系附属第二医院第二临床医学院、附属第二医院、育英儿童医院基础医学院（机能实验教学中心）_病原生物学与免疫学系_医学寄生虫学
通讯作者	Wen, Jin-Sheng
作者单位	1.Department of Microbiology and Immunology,Wenzhou Medical College,China; 2.Department of Clinical Laboratory,Second Affiliated Hospital,Wenzhou Medical College,China; 3.Department of Parasitology,Wenzhou Medical College,China; 4.Department of Microbiology,Zhongshan Medical School,Sun Yat-Sen University,China; 5.Department of Clinical Laboratory,People's Hospital of Ruian,China
第一作者单位	病原生物学与免疫学系; 附属第二医院
通讯作者单位	病原生物学与免疫学系
第一作者的第一单位	病原生物学与免疫学系
推荐引用方式 GB/T 7714	Duan, Zhi-Liang,Wang, Zhi-Bin,Guo, Jiang-Long,et al. Two novel squamous cell carcinoma antigen-derived HLA-A*0201-binding peptides induce in vitro and in vivo CD8(+) cytotoxic T lymphocyte responses[J]. INTERNATIONAL JOURNAL OF ONCOLOGY,2013,42(4):1482-1492.
APA	Duan, Zhi-Liang., Wang, Zhi-Bin., Guo, Jiang-Long., Liu, Wen-Quan., Hu, Jun., ... & Wen, Jin-Sheng. (2013). Two novel squamous cell carcinoma antigen-derived HLA-A*0201-binding peptides induce in vitro and in vivo CD8(+) cytotoxic T lymphocyte responses. INTERNATIONAL JOURNAL OF ONCOLOGY, 42(4), 1482-1492.
MLA	Duan, Zhi-Liang,et al."Two novel squamous cell carcinoma antigen-derived HLA-A*0201-binding peptides induce in vitro and in vivo CD8(+) cytotoxic T lymphocyte responses".INTERNATIONAL JOURNAL OF ONCOLOGY 42.4(2013):1482-1492.