Pulsed galvanostatic control of a solid-contact ion-selective electrode for potentiometric biosensing of microcystin-LR

doi:10.1016/j.snb.2016.02.121

科研成果详情

题名	Pulsed galvanostatic control of a solid-contact ion-selective electrode for potentiometric biosensing of microcystin-LR
作者	Yu, Nana 1,2; Ding, Jiawang 1; Wang, Wenwei 2; Wang, Xuedong 2; Qin, Wei 1
发表日期	2016-07
发表期刊	SENSORS AND ACTUATORS B-CHEMICAL 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Chronopotentiometry Solid-contact ion-selective electrodes Protein phosphatases Microcystin-LR
其他关键词	LABEL-FREE ; MEMBRANE ELECTRODES ; SENSITIVE DETECTION ; INHIBITION ASSAY ; ELECTROCHEMICAL DETECTION ; PROTEIN PHOSPHATASES ; ENZYME ; WATER ; QUANTIFICATION ; IMMUNOSENSOR
摘要	We report here on the development of a chronopotentiometric assay for microcystin-LR based on enzymatic inhibition. The inhibition of protein phosphatase by microcystin-LR can be sensed potentiometrically by using 4-nitrophenyl phosphate as an enzyme substrate. A solid-contact ion-selective electrode (ISE) with poly(3,4-ethylenedioxythiophene)-poly(styrenesulfonate) as a transduction layer has been designed for potentiometric biosensing using the pulsed galvanastatic technique. By applying an anodic current, the enzymatic generated p-nitrophenol can be extracted into the polymeric membrane with tetradodecylammonium tetrakis(4-chlorophenyl)-borate to produce the chronopotentiometric signal. Meanwhile, a controlled voltage was applied to refresh the membrane for multiple consecutive measurements. The proposed potentiometric assay showed a linear response for microcystin-LR in the range 1-100 mu g/L with a detection limit of 0.5 mu g/L (3 sigma). We believe that the proposed method can be employed for sensitive, rapid and reliable determination of analytes involved in enzyme inhibition. (C) 2016 Elsevier B.V. All rights reserved.
资助项目	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [21207156, 21575158, 41176081]; Youth Innovation Promotion Association of CAS [2013139]; Taishan Scholar Program of Shandong Province; Zhejiang Provincial Natural Science FoundationNatural Science Foundation of Zhejiang Province [LY14B070013]; Public Beneficial Project [2015C33306]
出版者	ELSEVIER SCIENCE SA
出版地	LAUSANNE
ISSN	0925-4005
卷号	230 页码:785-790
DOI	10.1016/j.snb.2016.02.121
页数	51
WOS类目	Chemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation
WOS研究方向	Chemistry ; Electrochemistry ; Instruments & Instrumentation
WOS记录号	WOS:000374329300100
收录类别	SCIE ; EI ; SCOPUS
EI入藏号	20161102083451
EI主题词	Ion selective electrodes
URL	查看原文
SCOPUSEID	2-s2.0-84960171661
通讯作者地址	[Ding, Jiawang]Key Laboratory of Coastal Environmental Processes and Ecological Remediation,Yantai Institute of Coastal Zone Research (YIC),Chinese Academy of Sciences (CAS),Shandong Provincial Key Laboratory of Coastal Environmental Processes,YICCAS,Yantai,264003,China
Scopus学科分类	Electronic, Optical and Magnetic Materials;Instrumentation;Condensed Matter Physics;Surfaces, Coatings and Films;Metals and Alloys;Electrical and Electronic Engineering;Materials Chemistry
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/5470
专题	公共卫生学院_环境科学系
通讯作者	Ding, Jiawang
作者单位	1.Key Laboratory of Coastal Environmental Processes and Ecological Remediation,Yantai Institute of Coastal Zone Research (YIC),Chinese Academy of Sciences (CAS),Shandong Provincial Key Laboratory of Coastal Environmental Processes,YICCAS,Yantai,264003,China; 2.Department of Environmental Sciences,Key Laboratory of Watershed Science and Health of Zhejiang Province,Wenzhou Medical University,Wenzhou,325035,China
第一作者单位	温州医科大学
推荐引用方式 GB/T 7714	Yu, Nana,Ding, Jiawang,Wang, Wenwei,et al. Pulsed galvanostatic control of a solid-contact ion-selective electrode for potentiometric biosensing of microcystin-LR[J]. SENSORS AND ACTUATORS B-CHEMICAL,2016,230:785-790.
APA	Yu, Nana, Ding, Jiawang, Wang, Wenwei, Wang, Xuedong, & Qin, Wei. (2016). Pulsed galvanostatic control of a solid-contact ion-selective electrode for potentiometric biosensing of microcystin-LR. SENSORS AND ACTUATORS B-CHEMICAL, 230, 785-790.
MLA	Yu, Nana,et al."Pulsed galvanostatic control of a solid-contact ion-selective electrode for potentiometric biosensing of microcystin-LR".SENSORS AND ACTUATORS B-CHEMICAL 230(2016):785-790.