科研成果详情

题名Validation of a Novel Fgf10(Cre-ERT2) Knock-in Mouse Line Targeting FGF10(Pos) Cells Postnatally
作者
发表日期2021-05-13
发表期刊FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY   影响因子和分区
语种英语
原始文献类型Article
关键词Fgf10 knock-in Cre line lipofibroblast adult lung lineage tracing
其他关键词M1 TRANSCRIPTION FACTOR ; LUNG DEVELOPMENT ; MAMMARY-GLAND ; LETHALITY ; MAINTENANCE ; EXPRESSION ; DEFECTS ; LIGAND ; OCT-6 ; MICE
摘要Fgf10 is a key gene during development, homeostasis and repair after injury. We previously reported a knock-in Fgf10(Cre-ERT2) line (with the Cre-ERT2 cassette inserted in frame with the start codon of exon 1), called thereafter Fgf10(Ki-v1), to target FGF10(Pos) cells. While this line allowed fairly efficient and specific labeling of FGF10(Pos) cells during the embryonic stage, it failed to target these cells after birth, particularly in the postnatal lung, which has been the focus of our research. We report here the generation and validation of a new knock-in Fgf10(Cre-ERT2) line (called thereafter Fgf10(Ki-v2)) with the insertion of the expression cassette in frame with the stop codon of exon 3. Fgf10(Ki-v2/+) heterozygous mice exhibited comparable Fgf10 expression levels to wild type animals. However, a mismatch between Fgf10 and Cre expression levels was observed in Fgf10(Ki-v2/+) lungs. In addition, lung and limb agenesis were observed in homozygous embryos suggesting a loss of Fgf10 functional allele in Fgf10(Ki-v2) mice. Bioinformatic analysis shows that the 3 ' UTR, where the Cre-ERT2 cassette is inserted, contains numerous putative transcription factor binding sites. By crossing this line with tdTomato reporter line, we demonstrated that tdTomato expression faithfully recapitulated Fgf10 expression during development. Importantly, Fgf10(Ki-v2) mouse is capable of significantly targeting FGF10(Pos) cells in the adult lung. Therefore, despite the aforementioned limitations, this new Fgf10(Ki-v2) line opens the way for future mechanistic experiments involving the postnatal lung.
资助项目Institute for Lung Health (ILH); German Research Foundation (DFG)German Research Foundation (DFG) [EL 931/4-1, KFO309 P7, SFB CRC1213]; Cardio-Pulmonary Institute (CPI, EXC 2026) [390649896]; German Center for Lung Research (DZL); DFGGerman Research Foundation (DFG)European Commission [KFO309 P7, BE4443/1-1, BE4443/4-1, BE4443/6-1, SFB1213]; Interventional Pulmonary Key Laboratory of Zhejiang Province; Interventional Pulmonology Innovation Subject of Zhejiang Province; National Nature Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81570075, 81770074]; Zhejiang Provincial Natural Science FoundationNatural Science Foundation of Zhejiang Province [LZ15H010001]; Zhejiang Provincial Science Technology Department Foundation [2015103253]; National Key Research and Development Program of China [2016YFC1304000]; CPI
出版者FRONTIERS MEDIA SA
出版地LAUSANNE
ISSN2296-634X
卷号9页码:671841
DOI10.3389/fcell.2021.671841
页数13
WOS类目Cell Biology ; Developmental Biology
WOS研究方向Cell Biology ; Developmental Biology
WOS记录号WOS:000654995400001
收录类别SCIE ; PUBMED ; SCOPUS
URL查看原文
Pubmed记录号34055804
PMC记录号PMC8155496
Scopus记录号2-s2.0-85107058004
通讯作者地址[Zhang, Jin-San]School of Pharmaceutical Sciences,Wenzhou Medical University,Wenzhou,China ; [Bellusci, Saverio]School of Pharmaceutical Sciences,Wenzhou Medical University,Wenzhou,China ; [El Agha, Elie]Department of Internal Medicine,Universities of Giessen and Marburg Lung Center (UGMLC),Cardio-Pulmonary Institute (CPI),German Center for Lung Research (DZL),Justus-Liebig University Giessen,Giessen,Germany
scopus学科分类Developmental Biology;Cell Biology
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符https://kms.wmu.edu.cn/handle/3ETUA0LF/5373
专题药学院(分析测试中心)
附属第二医院
附属第一医院
第二临床医学院、附属第二医院、育英儿童医院
第一临床医学院(信息与工程学院)、附属第一医院_内科学_呼吸与危重症医学科
通讯作者Zhang, Jin-San; El Agha, Elie; Bellusci, Saverio
作者单位
1.School of Pharmaceutical Sciences,Wenzhou Medical University,Wenzhou,China;
2.Key Laboratory of Interventional Pulmonology of Zhejiang Province,Department of Pulmonary and Critical Care Medicine,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China;
3.Department of Internal Medicine,Universities of Giessen and Marburg Lung Center (UGMLC),Cardio-Pulmonary Institute (CPI),German Center for Lung Research (DZL),Justus-Liebig University Giessen,Giessen,Germany;
4.Institute for Lung Health (ILH),Giessen,Germany;
5.National Key Clinical Specialty of Pediatric Respiratory Medicine,Discipline of Pediatric Respiratory Medicine,Institute of Pediatrics,The Second Affiliated Hospital of Wenzhou Medical University,Wenzhou,China
第一作者单位药学院(分析测试中心);  附属第一医院;  第一临床医学院(信息与工程学院)、附属第一医院
通讯作者单位药学院(分析测试中心)
第一作者的第一单位药学院(分析测试中心)
推荐引用方式
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Chu, Xuran,Taghizadeh, Sara,Vazquez-Armendariz, Ana Ivonne,et al. Validation of a Novel Fgf10(Cre-ERT2) Knock-in Mouse Line Targeting FGF10(Pos) Cells Postnatally[J]. FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY,2021,9:671841.
APA Chu, Xuran., Taghizadeh, Sara., Vazquez-Armendariz, Ana Ivonne., Herold, Susanne., Chong, Lei., ... & Bellusci, Saverio. (2021). Validation of a Novel Fgf10(Cre-ERT2) Knock-in Mouse Line Targeting FGF10(Pos) Cells Postnatally. FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY, 9, 671841.
MLA Chu, Xuran,et al."Validation of a Novel Fgf10(Cre-ERT2) Knock-in Mouse Line Targeting FGF10(Pos) Cells Postnatally".FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY 9(2021):671841.

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