2-BFI attenuates experimental autoimmune encephalomyelitis-induced spinal cord injury with enhanced B-CK, CaATPase, but reduced calpain activity

doi:10.1016/j.bbrc.2011.02.018

科研成果详情

题名	2-BFI attenuates experimental autoimmune encephalomyelitis-induced spinal cord injury with enhanced B-CK, CaATPase, but reduced calpain activity
作者	Wang, Pei 1,2; Wang, Zhao-Wei1,2; Lin, Fu-Hong1,2; Han, Zhao1,2; Hou, Sheng-Tao 1,2,3; Zheng, Rong-Yuan1,2
发表日期	2011-03-04
发表期刊	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 影响因子和分区
语种	英语
原始文献类型	Article
关键词	2-BFI Brain-creatine kinase CaATPase Calpain Imidazoline 2 receptor Experimental autoimmune encephalomyelitis Multiple sclerosis
其他关键词	ISCHEMIC MOUSE-BRAIN ; MULTIPLE-SCLEROSIS ; CEREBRAL-ISCHEMIA ; GLUTAMATE TOXICITY ; NEURONAL DEATH ; EXPRESSION ; NEURODEGENERATION ; DEMYELINATION ; INFLAMMATION ; MECHANISMS
摘要	The lack of disease-modifying pharmacological agents for effective treatment of multiple sclerosis (MS) still represents a large and urgent unmet medical need. Our previous studies showed that ligands to type 2 imidazoline receptors (I2R) were effective in protecting spinal cord injury caused by experimental autoimmune encephalomyelitis (EAE), a mouse model of MS. In this study, we further examined the protective property of a very selective ligand of I2R, 2-(2-benzofuranyl) 2-imidazoline (2-BFI) against EAE. Importantly, a mechanism of 2-BFI-mediated protection was investigated which possibly involves an I2R binding protein, brain-creatine kinase (B-CK), as well as CaATPase and calpain. The enzymatic activity of B-CK and CaATPase was significantly reduced in EAE injured spinal cord. Reduction of B-CK activity in EAE spinal cord may lead to energy reduction and dysfunction in cellular calcium homeostasis. Increased intracellular calcium evokes elevation of calpain activity occurring in EAE spinal cord which causes further tissue damage. Indeed, EAE injured spinal cord showed significant reduction in CaATPase and increase calpain activities. Remarkably, spinal cord tissue from mice treated daily with 2-BFI during the progression of EAE significantly restored B-CK and CaATPase enzymatic activities and showed no induction in calpain activity. Moreover, EAE spinal cord from 2-BFI treated mice also demonstrated better preservation of myelin; reduced axonal injury, as evidenced by the lower level of beta-APP expression, and above all, highly improved neurobehavioral scores (p < 0.01; n = 10). These findings suggest that 2-BFI can be further developed as a therapeutic drug for MS treatment. Crown Copyright (C) 2011 Published by Elsevier Inc. All rights reserved.
资助项目	Chinese National Natural Science FoundationNational Natural Science Foundation of China (NSFC) [81070960]; Zhejiang Provincial Natural Science [Y207494]; Zhejiang Key Subject (Pharmacology and Biochemical Pharmaceutics); 3010 grant
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
出版地	SAN DIEGO
ISSN	0006-291X
EISSN	1090-2104
卷号	406 期号:1 页码:152-157
DOI	10.1016/j.bbrc.2011.02.018
页数	6
WOS类目	Biochemistry & Molecular Biology ; Biophysics
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics
WOS记录号	WOS:000288302100028
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	21303658
SCOPUSEID	2-s2.0-79952185502
通讯作者地址	[Zheng, Rong-Yuan]First Affiliated Hosp, Dept Neurol, 2 Fuxue Lane, Wenzhou City 325000, Zhejiang, Peoples R China.
引用统计	被引频次：18[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/5358
专题	药学院（分析测试中心）_实验神经生物研究所
通讯作者	Zheng, Rong-Yuan
作者单位	1.First Affiliated Hosp, Dept Neurol, Wenzhou City 325000, Zhejiang, Peoples R China; 2.Wenzhou Med Coll, Res Inst Expt Neurobiol, Wenzhou City 325000, Zhejiang, Peoples R China; 3.Natl Res Council Canada, Inst Biol Sci, Ottawa, ON K1A 0R6, Canada
第一作者单位	药学院（分析测试中心）_实验神经生物研究所
推荐引用方式 GB/T 7714	Wang, Pei,Wang, Zhao-Wei,Lin, Fu-Hong,et al. 2-BFI attenuates experimental autoimmune encephalomyelitis-induced spinal cord injury with enhanced B-CK, CaATPase, but reduced calpain activity[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2011,406(1):152-157.
APA	Wang, Pei, Wang, Zhao-Wei, Lin, Fu-Hong, Han, Zhao, Hou, Sheng-Tao, & Zheng, Rong-Yuan. (2011). 2-BFI attenuates experimental autoimmune encephalomyelitis-induced spinal cord injury with enhanced B-CK, CaATPase, but reduced calpain activity. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 406(1), 152-157.
MLA	Wang, Pei,et al."2-BFI attenuates experimental autoimmune encephalomyelitis-induced spinal cord injury with enhanced B-CK, CaATPase, but reduced calpain activity".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 406.1(2011):152-157.