Sperm cryopreservation of yellow drum Nibea albiflora: A special emphasis on post-thaw sperm quality

doi:10.1016/j.aquaculture.2012.09.017

科研成果详情

题名	Sperm cryopreservation of yellow drum Nibea albiflora: A special emphasis on post-thaw sperm quality
作者	Dai, Tongren 1; Zhao, Enhui1,2; Lu, Gang 1; Che, Kai 1; He, Qiutao 1; Lu, Yonglin 3; Fang, Qiongshan 4; Wang, Hansheng 4; Zheng, Leyun 4; Li, Suping 4; Huang, Changjiang 1; Dong, Qiaoxiang1
发表日期	2012-11-24
发表期刊	AQUACULTURE 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Yellow drum Nibea albiflora Sperm cryopreservation Sperm quality Cholesterol Sphingomyelin
其他关键词	FERTILIZATION CAPACITY ; SPERMATOZOA ; MOTILITY ; DNA
摘要	This study was intended to develop an optimal sperm cryopreservation protocol for yellow drum (Nibea albiflora), a commercially important fish species in East Asia. Specifically, we evaluated cryoprotectant type, concentration, and cooling rate and found that the highest fertilization (56-61%) and hatch (42-47%) can be obtained from yellow drum sperm suspended in 5% ethylene glycol or 10% dimethyl sulfoxide, cooled at 220 degrees C/min, thawed in 40 degrees C water bath for 7 s, and fertilized with fresh (non-frozen) eggs at a sperm-to-egg ratio of 10(7). We also compared other sperm quality parameters such as sperm motility, motility duration, membrane integrity, mitochondrial membrane potential, and DNA fragmentation between fresh and thawed sperm samples. Although cryopreservation resulted in a significant increase in DNA fragmentation and significant decreases in the remaining parameters, the magnitude of these changes was small when compared to the 2000 to 3000-fold reduction in fertilization/hatch capability of thawed sperm. These findings suggest possible disruption of signaling pathways that cannot be detected by conventional quality assays. Further analysis of sperm membrane lipid composition revealed a significant negative correlation between sperm quality and cholesterol-to-sphingomyelin ratio. These findings indicate that lipid rafts could be the main targets for cryodamage-induced decrease in fertilization capability of thawed sperm. Future studies are necessary to further explore the role of lipid rafts in cryopreservation-mediated damage in fish sperm. (C) 2012 Elsevier B.V. All rights reserved.
资助项目	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [30800845]; Natural Science Fund for Distinguished Young Scholars of Zhejiang Province [R3100105]; Public Welfare Project from the Science Technology Department of Zhejiang Province [2011C22031]; Resource Conservation Program of Special Aquatic Species in Fujian Province [2008N2004]
出版者	ELSEVIER SCIENCE BV
出版地	AMSTERDAM
ISSN	0044-8486
EISSN	1873-5622
卷号	368 页码:82-88
DOI	10.1016/j.aquaculture.2012.09.017
页数	7
WOS类目	Fisheries ; Marine & Freshwater Biology
WOS研究方向	Fisheries ; Marine & Freshwater Biology
WOS记录号	WOS:000310572900011
收录类别	SCIE ; SCOPUS
URL	查看原文
SCOPUSEID	2-s2.0-84867235179
通讯作者地址	[Dong, Qiaoxiang]Wenzhou Med Coll, Inst Watershed Sci & Environm Ecol, Zhejiang Prov Key Lab Technol & Applicat Model Or, Wenzhou 325035, Peoples R China.
TOP期刊	TOP期刊
引用统计	被引频次：12[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/5354
专题	温州医科大学
通讯作者	Dong, Qiaoxiang
作者单位	1.Wenzhou Med Coll, Inst Watershed Sci & Environm Ecol, Zhejiang Prov Key Lab Technol & Applicat Model Or, Wenzhou 325035, Peoples R China; 2.Lishui Peoples Hosp, Lishui 323000, Peoples R China; 3.Wenzhou Fisheries Technol Extens Serv, Wenzhou 325035, Peoples R China; 4.Fujian Fisheries Res Inst, Xiamen 361012, Peoples R China
第一作者单位	温州医科大学
通讯作者单位	温州医科大学
第一作者的第一单位	温州医科大学
推荐引用方式 GB/T 7714	Dai, Tongren,Zhao, Enhui,Lu, Gang,et al. Sperm cryopreservation of yellow drum Nibea albiflora: A special emphasis on post-thaw sperm quality[J]. AQUACULTURE,2012,368:82-88.
APA	Dai, Tongren., Zhao, Enhui., Lu, Gang., Che, Kai., He, Qiutao., ... & Dong, Qiaoxiang. (2012). Sperm cryopreservation of yellow drum Nibea albiflora: A special emphasis on post-thaw sperm quality. AQUACULTURE, 368, 82-88.
MLA	Dai, Tongren,et al."Sperm cryopreservation of yellow drum Nibea albiflora: A special emphasis on post-thaw sperm quality".AQUACULTURE 368(2012):82-88.