姜黄素对气道重塑哮喘大鼠尾加压素Ⅱ的影响

科研成果详情

题名	姜黄素对气道重塑哮喘大鼠尾加压素Ⅱ的影响
其他题名	Effect of curcumin on expression of urotensinⅡ in airway remodeling in asthmatic rats
作者	杨能力1; 梁亚峰2; 吕雅1; 李前君 1; 王均炉1
发表日期	2013
发表期刊	中国药理学与毒理学杂志影响因子和分区
语种	中文
原始文献类型	学术期刊
关键词	尾加压素哮喘气道重塑姜黄素
摘要	目的观察姜黄素对哮喘大鼠气道重塑及尾加压素Ⅱ(UⅡ)表达的影响。方法大鼠间隔7d共2次ip给以卵清蛋白(OVA)/Al(OH)3,第15天雾化吸入OVA激发,隔天1次,制备哮喘模型。按照分组,分别于雾化吸入OVA前30min,ig给予姜黄素50,100和200mg·kg-1,连续6周。图像分析技术测量支气管壁厚度和平滑肌厚度,ELISA法测定大鼠支气管肺泡灌洗液(BALF)和血清中UⅡ的含量,RTPCR检测肺组织UⅡmRNA表达。结果与正常对照组比较,哮喘模型组支气管壁厚度及平滑肌厚度均明显升高,分别增加了1.8和1.9倍(P<0.01)。与哮喘模型组比较,姜黄素组支气管壁厚度及平滑肌厚度均明显降低,分别减少了11.8%,27.8%,32.5%和16.6%,29.2%和38.4%(P<0.01)。哮喘模型组BALF和血清中UⅡ含量显著高于正常对照组,分别增加了16.9和11.9倍(P<0.01);与哮喘模型组相比,姜黄素组BALF和血清中UⅡ含量显著降低,分别减少了21.11%,50.5%,65.4%和26.92%,50.1%和60.8%(P<0.01)。与正常对照组相比,哮喘模型组肺组织UⅡmRNA表达增加了3.3倍(P<0.01);姜黄素组肺组织UⅡmRNA含量较哮喘模型组分别降低了30.3%,37.1%和52.8%(P<0.01)。结论姜黄素可以减少哮喘大鼠BALF和血清中UⅡ含量及肺组织UⅡmRNA的表达,其抑制哮喘气道重塑可能与降低UⅡ的表达有关。
其他摘要	OBJECTIVE To observe the effect of curcumin on the expression of urotensinⅡ(UⅡ) in airway remodeling in asthmatic rats. METHODS The rat asthma models were sensitized with ip of 1 mg OVA with 100 μg aluminum hydroxide dissolved in 1.5 ml saline on d 1 and d 8. They were then challenged with 1% OVA aerosol every other day for 30 min from d 15 for 6 weeks. Curcumin 50, 100 and 200 mg·kg-1 was ig given 30 min before challenge from day 15 for 6 weeks, respectively. The total bronchial wall thickness and the airway smooth muscle thickness were measured by image analysis system. The contents of UⅡ in bronchoalveolar lavage fluid (BALF) and serum were detected using ELISA. UⅡ mRNA contents were detected by RT-PCR. RESULTS Compared with normal control group, the total bronchial wall thickness and the airway smooth musle thickness of asthma model group were significantly thicker (increased 2.8 and 2.9 times)(P<0.01), the total bronchial wall thickness and the airway smooth musle thickness of curcumin group were significantly thinner than in asthma model group(decreased by 11.8%, 27.8%, 32.5%, and 16.6%, 29.2%, 38.4%)(P<0.01). Compared with normal control group, the contents of UⅡ in BALF and serum of asthma model group were higher (increased 17.9 and 12.9 times, respectively)(P<0.01), while those of curcumin group were significantly lower compared with asthma model group(decreased by 21.11%, 50.5%, 65.4%, and 26.92%, 50.1%, 60.8%, respectively)(P<0.01). The UⅡmRNA contents in the curcumin groups were significantly lower than those in the asthma model group(decreased by 30.3%, 37.1%, 52.8%)(P<0.01), but those in asthma model group were higher than in normal control group(increased 4.3 times)(P<0.01). CONCLUSION Curcumin can decrease the contents of UⅡ in BALF and serum, and the expression of UⅡ mRNA of asthmatic rats, which is associated with alleviated formation of airway remodeling.
ISSN	1000-3002
卷号	27 期号:05 页码:814-817
页数	4
收录类别	CNKI ; 维普 ; 北大核心 ; CSCD
学科领域	医药、卫生 ; 中国医学 ; 药学
URL	查看原文
CSCD记录号	CSCD:4963839
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/53434
专题	附属第一医院附属第二医院第二临床医学院，附属第二医院、育英儿童医院
作者单位	1.温州医科大学附属第一医院麻醉科; 2.温州医科大学附属第二医院育英儿童医院儿童重症监护室
第一作者单位	附属第一医院; 附属第一医院_麻醉科
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	杨能力,梁亚峰,吕雅,等. 姜黄素对气道重塑哮喘大鼠尾加压素Ⅱ的影响[J]. 中国药理学与毒理学杂志,2013,27(05):814-817.
APA	杨能力, 梁亚峰, 吕雅, 李前君, & 王均炉. (2013). 姜黄素对气道重塑哮喘大鼠尾加压素Ⅱ的影响. 中国药理学与毒理学杂志, 27(05), 814-817.
MLA	杨能力,et al."姜黄素对气道重塑哮喘大鼠尾加压素Ⅱ的影响".中国药理学与毒理学杂志 27.05(2013):814-817.

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