MAPK通路抑制剂对三七皂苷单体R1减轻大鼠低氧高二氧化碳性肺血管收缩的影响

doi:10.3969/j.issn.1000-4718.2013.10.007

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题名	MAPK通路抑制剂对三七皂苷单体R1减轻大鼠低氧高二氧化碳性肺血管收缩的影响
其他题名	Role of MAPK inhibition in decrease of hypoxia hypercapnia-induced pulmonary vasoconstriction mediated by Panax notoginseng saponin R1 in rats
作者	马迎春1; 陈海娥 1; 王淑君 2; 黄林静1; 何金波 1; 应磊1,3; 陈锡文4; 王万铁1,3
发表日期	2013-10-15
发表期刊	中国病理生理杂志影响因子和分区
语种	中文
原始文献类型	学术期刊
关键词	三七皂苷单体R1 低氧高碳酸血症 p38丝裂原激活蛋白激酶类细胞外信号调节激酶1/2 大鼠
其他关键词	Panax notoginseng saponin R1 ; Hypoxia ; Hypercapnia ; p38 mitogen-activated protein kinases ; Extracellular signal-regulated kinase 1/2 ; Rats
摘要	目的:探讨三七皂苷单体R1对低氧高二氧化碳性肺血管收缩(HHPV)的影响及其与MAPK信号通路的关系。方法:采用大鼠离体肺动脉环灌流模型,随机将其分为:常氧组(N组);低氧高二氧化碳组(H组);低氧高二氧化碳+DMSO组(HD组);低氧高二氧化碳+R1组,又分为低浓度组(RL组)、中浓度组(RM组)和高浓度组(RH组);低氧高二氧化碳+SB203580组(S组);低氧高二氧化碳+U0126组(U组);低氧高二氧化碳+R1+SB203580组(RS组);低氧高二氧化碳+R1+U0126组(RU组)。观察二级肺动脉环在常氧及急性低氧高二氧化碳条件下的张力变化。在急性低氧高二氧化碳条件下,观察不同浓度R1孵育及最佳浓度R1分别与SB203580、U0126联合孵育对HHPV三期变化的影响,按照低氧高二氧化碳反应性测定方法测定血管张力变化值。结果:在急性低氧高二氧化碳条件下:(1)二级肺动脉呈现双相性收缩变化,与N组相比显著差异(P<0.05或P<0.01);(2)HD组和RL组能明显缓解HHPV的I期快速收缩,逆转II期持续收缩,RM组作用不明显,RH组增强HHPV的I期快速收缩和II期持续收缩。与HD组比较,RL和RH组有显著差异(P<0.05或P<0.01);(3)RS组和RU组收缩峰值较HD组均明显下降,II期持续收缩逆转为舒张状态。与RL组相比,RS组和RU组HHPV显著缓解(P<0.05或P<0.01)。与S组和U组相比,RS组和RU组HHPV显著缓解(P<0.05或P<0.01)。结论:低浓度(8 mg/L)三七皂苷单体R1可减轻大鼠急性HHPV,其机制可能与MAPK(p38 MAPK和ERK1/2)信号通路的参与有关。
其他摘要	AIM:To investigate the role of Panax notoginseng saponin R1 in the pathological process of hypo-xia hypercapnia-induced pulmonary vasoconstriction (HHPV) and to observe the relationship with MAPK signal pathway in rats. METHODS:The model of pulmonary artery ring perfusion in vitro was used, and the rings were divided randomly into the following groups: normoxia group (N group); hypoxia hypercapnia group (H group); H+DMSO incubation group (HD group); H+R1 group, which was divided into 3 subgroups: low-concentration R1 group (RL group), middle-concentration R1 group (RM group) and high-concentration R1 group (RH group); H+SB203580 (p38 MAPK inhibitor) incubation group (S group); H+U0126 (ERK1/2 inhibitor) incubation group (U group); H+R1+SB203580 incubation group (RS group); H+R1+U0126 (RU group). Under acute hypoxia hypercapnia condition, the effects of different concentrations of R1 or R1 at the optimal concentration combined with U0126 or SB203580 on the 3 stages of HHPV were observed. At the same time, the changes of ring tension were recorded via the method of hypoxia hypercapnia condition reactivity. RESULTS:Under the hypoxia hypercapnia condition, a biphasic pulmonary artery contractile response (phase I acute vasoconstriction, phase I vasodilation and phase II persistent vasoconstriction) in the secondary pulmonary artery rings was observed. The treatments in HD group and RL group distinctly relieved the early phase I acute vasoconstriction of HHPV and reversed the phase II persistent vasoconstriction, but the effect in RM group was not obvious. The treatment in RH group enhanced both the early phase I acute vasoconstriction and the phase II persistent vasoconstriction of HHPV. RL and RH groups had significant differences compared with HD group. In contrast to HD group, the values of systolic peak in RS and RU groups decreased dramatically, and the phase II persistent vasoconstriction reversed to relaxation state. The HHPV in RS and RU groups was significantly relieved as compared with RL group. The HHPV in RS and RU groups was relieved as compared with S group and U group. CONCLUSION:R1 at concentration of 8 mg/L relieves acute HHPV in rats. The mechanism may be associated with p38 MAPK and ERK1/2 signaling pathway.
资助项目	浙江省科技攻关计划项目（No.2006C33073）;浙江省中医药科技计划重点资助项目（No.2008ZA017）;浙江省中医药重点学科建设计划项目（No.2012-XK-A28）
ISSN	1000-4718
卷号	29 期号:10 页码:1764-1770
DOI	10.3969/j.issn.1000-4718.2013.10.007
页数	7
收录类别	CNKI ; 万方 ; 北大核心 ; PKU ; CSCD ; ISTIC
学科领域	医药、卫生 ; 基础医学
URL	查看原文
CSCD记录号	CSCD:4964249
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/53164
专题	基础医学院（机能实验教学中心）实验动物中心
作者单位	1.温州医科大学基础医学院病理生理学教研室; 2.赤峰上京内分泌专科医院; 3.温州医科大学缺血-再灌注损伤研究所; 4.温州医科大学实验动物中心
第一作者单位	基础医学院（机能实验教学中心）
第一作者的第一单位	基础医学院（机能实验教学中心）
推荐引用方式 GB/T 7714	马迎春,陈海娥,王淑君,等. MAPK通路抑制剂对三七皂苷单体R1减轻大鼠低氧高二氧化碳性肺血管收缩的影响[J]. 中国病理生理杂志,2013,29(10):1764-1770.
APA	马迎春., 陈海娥., 王淑君., 黄林静., 何金波., ... & 王万铁. (2013). MAPK通路抑制剂对三七皂苷单体R1减轻大鼠低氧高二氧化碳性肺血管收缩的影响. 中国病理生理杂志, 29(10), 1764-1770.
MLA	马迎春,et al."MAPK通路抑制剂对三七皂苷单体R1减轻大鼠低氧高二氧化碳性肺血管收缩的影响".中国病理生理杂志 29.10(2013):1764-1770.

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