High-mobility group box 2 is associated with prognosis of glioblastoma by promoting cell viability, invasion, and chemotherapeutic resistance

doi:10.1093/neuonc/not078

科研成果详情

题名	High-mobility group box 2 is associated with prognosis of glioblastoma by promoting cell viability, invasion, and chemotherapeutic resistance
作者	Wu, Zhe Bao 1,2; Cai, Lin2; Lin, Shao Jian 2; Xiong, Zhen Kun 2; Lu, Jiang Long 2; Mao, Ying 1; Yao, Yu 1; Zhou, Liang Fu 1
发表日期	2013-09
发表期刊	NEURO-ONCOLOGY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	glioblastoma high-mobility group box 2 prognosis invasion temozolomide
其他关键词	BREAST-CANCER ; MATRIX METALLOPROTEINASES ; TISSUE INHIBITORS ; HEPATOCELLULAR-CARCINOMA ; ADJUVANT TEMOZOLOMIDE ; INCREASED EXPRESSION ; PROTEINS ; CISPLATIN ; HMGB1 ; METHYLATION
摘要	The expression profile of high-mobility group box 2 (HMGB2) in patients with glioblastoma multiforme (GBM) and its clinical signature with underlying mechanisms were not fully explored. HMGB2 protein levels were measured in 51 GBM patients by immunohistochemical studies. To clarify the precise role of HMGB2 on cell invasion and viability of 3 GBM cell lines, we did in vitro and in vivo analyses with lentivirus vectors and small interfering RNA. Transwell invasion assays and wound-healing assays were used to analyze the invasion of GBM cells. Expression of p53 and matrix metalloproteinase 2/tissue inhibitors of metalloproteinase 2 (MMP2/TIMP2) protein was analyzed by Western blot. HMGB2 protein expression was significantly higher in GBM than in controlled brain tissues (P .0001). HMGB2 overexpression was significantly correlated with shorter overall survival time, which was the only independent prognostic factor for overall survival in a multivariate analysis (P .017). HMGB2 knockdown by small interfering RNA decreased cell viability and invasion in vitro and significantly decreased tumor volume in vivo, which might be involved in the change of p53 expression and the balance of MMP2/TIMP2. Moreover, silencing of HMGB2 could significantly increase the sensitivity of GBM cells to temozolomide chemotherapy. Our present data suggest that HMGB2 expression is a significant prognostic factor and might play an important role in cell invasion and temozolomide-induced chemotherapeutic sensitivity of GBM. This study highlights the importance of HMGB2 as a novel prognostic marker and an attractive therapeutic target of GBM.
资助项目	Shanghai Science and Technology Research Program [10JC1402201]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81271523]; Zhejiang Provincial Natural Science Foundation of ChinaNatural Science Foundation of Zhejiang Province [R2091137]; Zhejiang Provincial Program for the Cultivation of High-level Innovative Health Talents
出版者	OXFORD UNIV PRESS INC
出版地	CARY
ISSN	1522-8517
EISSN	1522-8517
卷号	15 期号:9 页码:1264-1275
DOI	10.1093/neuonc/not078
页数	12
WOS类目	Oncology ; Clinical Neurology
WOS研究方向	Oncology ; Neurosciences & Neurology
WOS记录号	WOS:000323617500016
收录类别	SCIE ; SCOPUS
URL	查看原文
PubMed ID	23828241
SCOPUSEID	2-s2.0-84883156379
自科自定义期刊分类	T3(B)类
通讯作者地址	[Zhou, Liang Fu]Department of Neurosurgery,Huashan Hospital,Fudan University,12 Wulumuqi Middle Road,China
Scopus学科分类	Oncology;Neurology (clinical);Cancer Research
TOP期刊	TOP期刊
引用统计	被引频次：32[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/5293
专题	附属第一医院
通讯作者	Zhou, Liang Fu
作者单位	1.Department of Neurosurgery,Huashan Hospital,Fudan University,12 Wulumuqi Middle Road,China; 2.Department of Neurosurgery,First Affiliated Hospital of Wenzhou Medical College,China
第一作者单位	附属第一医院
推荐引用方式 GB/T 7714	Wu, Zhe Bao,Cai, Lin,Lin, Shao Jian,et al. High-mobility group box 2 is associated with prognosis of glioblastoma by promoting cell viability, invasion, and chemotherapeutic resistance[J]. NEURO-ONCOLOGY,2013,15(9):1264-1275.
APA	Wu, Zhe Bao., Cai, Lin., Lin, Shao Jian., Xiong, Zhen Kun., Lu, Jiang Long., ... & Zhou, Liang Fu. (2013). High-mobility group box 2 is associated with prognosis of glioblastoma by promoting cell viability, invasion, and chemotherapeutic resistance. NEURO-ONCOLOGY, 15(9), 1264-1275.
MLA	Wu, Zhe Bao,et al."High-mobility group box 2 is associated with prognosis of glioblastoma by promoting cell viability, invasion, and chemotherapeutic resistance".NEURO-ONCOLOGY 15.9(2013):1264-1275.