Proteomic Analysis and qRT-PCR Verification of Temperature Response to Arthrospira (Spirulina) platensis

doi:10.1371/journal.pone.0083485

科研成果详情

题名	Proteomic Analysis and qRT-PCR Verification of Temperature Response to Arthrospira (Spirulina) platensis
作者	Wang Huili1; Zhao Xiaokai 1; Lin Meili 1; Dahlgren, Randy A.2; Chen Wei 1; Zhou Jaiopeng 1; Xu Chengyang 1; Jin Chunlei1; Xu Yi1; Wang Xuedong 3; Ding Li 1; Bao Qiyu1
发表日期	2013-12-12
发表期刊	PLOS ONE 影响因子和分区
语种	英语
原始文献类型	Article
其他关键词	HEAT-SHOCK-PROTEIN ; STRESS ; CYANOBACTERIUM ; EXPRESSION ; GENE ; RNA ; ACCLIMATION ; TOLERANCE ; HSPA ; DNA
摘要	Arthrospira (Spirulina) platensis (ASP) is a representative filamentous, non-N-2-fixing cyanobacterium that has great potential to enhance the food supply and possesses several valuable physiological features. ASP tolerates high and low temperatures along with highly alkaline and salty environments, and can strongly resist oxidation and irradiation. Based on genomic sequencing of ASP, we compared the protein expression profiles of this organism under different temperature conditions (15 degrees C, 35 degrees C and 45 degrees C) using 2-DE and peptide mass fingerprinting techniques. A total of 122 proteins having a significant differential expression response to temperature were retrieved. Of the positively expressed proteins, the homologies of 116 ASP proteins were found in Arthrospira (81 proteins in Arthrospira platensis str. Paraca and 35 in Arthrospira maxima CS-328). The other 6 proteins have high homology with other microorganisms. We classified the 122 differentially expressed positive proteins into 14 functions using the COG database, and characterized their respective KEGG metabolism pathways. The results demonstrated that these differentially expressed proteins are mainly involved in post-translational modification (protein turnover, chaperones), energy metabolism (photosynthesis, respiratory electron transport), translation (ribosomal structure and biogenesis) and carbohydrate transport and metabolism. Others proteins were related to amino acid transport and metabolism, cell envelope biogenesis, coenzyme metabolism and signal transduction mechanisms. Results implied that these proteins can perform predictable roles in rendering ASP resistance against low and high temperatures. Subsequently, we determined the transcription level of 38 genes in vivo in response to temperature and identified them by qRT-PCR. We found that the 26 differentially expressed proteins, representing 68.4% of the total target genes, maintained consistency between transcription and translation levels. The remaining 12 genes showed inconsistent protein expression with transcription level and accounted for 31.6% of the total target genes.
资助项目	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31071115, 31270548]; Key Project of Environmental Protection Department of Zhejiang Province, China [2012B014]
出版者	PUBLIC LIBRARY SCIENCE
出版地	SAN FRANCISCO
ISSN	1932-6203
卷号	8 期号:12 页码:e83485.
DOI	10.1371/journal.pone.0083485
页数	23
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
WOS记录号	WOS:000328731800121
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	24349519
PMC记录号	PMC3861494
SCOPUSEID	2-s2.0-84892578910
通讯作者地址	[Wang Xuedong]Wenzhou Med Univ, Sch Environm Sci & Publ Hlth, Wenzhou, Peoples R China.
Scopus学科分类	Multidisciplinary
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/5241
专题	检验医学院（生命科学学院、生物学实验教学中心）仁济学院_环境与公共卫生学部
通讯作者	Wang Xuedong
作者单位	1.Wenzhou Med Univ, Sch Life Sci, Zhejiang Prov Key Lab Med Genet, Inst Biomed Informat, Wenzhou, Peoples R China; 2.Univ Calif Davis, Dept Land Air & Water Resources, Davis, CA 95616 USA; 3.Wenzhou Med Univ, Sch Environm Sci & Publ Hlth, Wenzhou, Peoples R China
第一作者单位	检验医学院（生命科学学院、生物学实验教学中心）
通讯作者单位	环境与公共卫生学部
第一作者的第一单位	检验医学院（生命科学学院、生物学实验教学中心）
推荐引用方式 GB/T 7714	Wang Huili,Zhao Xiaokai,Lin Meili,et al. Proteomic Analysis and qRT-PCR Verification of Temperature Response to Arthrospira (Spirulina) platensis[J]. PLOS ONE,2013,8(12):e83485..
APA	Wang Huili., Zhao Xiaokai., Lin Meili., Dahlgren, Randy A.., Chen Wei., ... & Bao Qiyu. (2013). Proteomic Analysis and qRT-PCR Verification of Temperature Response to Arthrospira (Spirulina) platensis. PLOS ONE, 8(12), e83485..
MLA	Wang Huili,et al."Proteomic Analysis and qRT-PCR Verification of Temperature Response to Arthrospira (Spirulina) platensis".PLOS ONE 8.12(2013):e83485..