A simple, rapid and low-cost staining method for gel-electrophoresis separated phosphoproteins via the fluorescent purpurin dye

doi:10.1039/c4an01334d

科研成果详情

题名	A simple, rapid and low-cost staining method for gel-electrophoresis separated phosphoproteins via the fluorescent purpurin dye
作者	Cong, Weitao1,4; Shen, Jiayi 1; Xuan, Yuanhu1; Zhu, Xinliang 1; Ni, Maowei 3; Zhu, Zhongxin 1,4; Hong, Guoying1,4; Lu, Xianghong 1; Jin, Litai1,2,4
发表日期	2014-12-31
发表期刊	ANALYST 影响因子和分区
语种	英语
原始文献类型	Article
其他关键词	EXTRACTION ; PROTEINS ; PHOSPHORYLATION ; IONS
摘要	A novel fluorescence detection method for phosphoproteins in 1-D and 2-D SDS-PAGE by using purpurin is developed in this study. Phosphoproteins as low as 4-8 ng could be specifically detected by purpurin within 60 min, and the detection limit is similar to or better than that of Pro-Q Diamond staining. Only 2 steps (staining and destaining) are needed for purpurin staining without requiring excessive fixing and washing steps, and for single use, $0.8 is enough for purpurin staining. By comprehensively comparing with Pro-Q Diamond staining, it is concluded that purpurin staining is a simple, rapid and low-cost staining method for a broad application to the research of phosphoproteins.
资助项目	National Key Scientific Instrument Development Program of China [2011YQ030139]; National Nature Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31300657]; Zhejiang Provincial Natural Science FoundationNatural Science Foundation of Zhejiang Province [LY13C050002, Y2100765]; Zhejiang Provincial Program for the Cultivation of High-level Innovative Health talents and the Science; Zhejiang Provincial Program for skill talents and technical innovation [2013R30004]; Qianjiang talents Program [QJD1202019]; Public Projects of Zhejiang Province [2013C37050]
出版者	ROYAL SOC CHEMISTRY
出版地	CAMBRIDGE
ISSN	0003-2654
EISSN	1364-5528
卷号	139 期号:23 页码:6104-6108
DOI	10.1039/c4an01334d
页数	5
WOS类目	Chemistry, Analytical
WOS研究方向	Chemistry
WOS记录号	WOS:000344523800006
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	25325196
SCOPUSEID	2-s2.0-84908409711
通讯作者地址	[Lu, Xianghong]School of Pharmaceutical Sciences,Key Laboratory of Biotechnology Pharmaceutical Engineering,Wenzhou Medical University,China
Scopus学科分类	Analytical Chemistry;Biochemistry;Environmental Chemistry;Spectroscopy;Electrochemistry
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/5010
专题	药学院（分析测试中心）其他_附属第六医院（丽水市人民医院）
通讯作者	Lu, Xianghong
作者单位	1.School of Pharmaceutical Sciences,Key Laboratory of Biotechnology Pharmaceutical Engineering,Wenzhou Medical University,China; 2.The Sixth Affiliated Hospital of Wenzhou Medical University,China; 3.Zhejiang Cancer Hospital,China; 4.Wenzhou Undersun Biotechnology Co Ltd.,China
第一作者单位	药学院（分析测试中心）
通讯作者单位	药学院（分析测试中心）
第一作者的第一单位	药学院（分析测试中心）
推荐引用方式 GB/T 7714	Cong, Weitao,Shen, Jiayi,Xuan, Yuanhu,et al. A simple, rapid and low-cost staining method for gel-electrophoresis separated phosphoproteins via the fluorescent purpurin dye[J]. ANALYST,2014,139(23):6104-6108.
APA	Cong, Weitao., Shen, Jiayi., Xuan, Yuanhu., Zhu, Xinliang., Ni, Maowei., ... & Jin, Litai. (2014). A simple, rapid and low-cost staining method for gel-electrophoresis separated phosphoproteins via the fluorescent purpurin dye. ANALYST, 139(23), 6104-6108.
MLA	Cong, Weitao,et al."A simple, rapid and low-cost staining method for gel-electrophoresis separated phosphoproteins via the fluorescent purpurin dye".ANALYST 139.23(2014):6104-6108.