Promoter hypermethylation of SOX11 promotes the progression of cervical cancer in vitro and in vivo

doi:10.3892/or.2019.6993

科研成果详情

题名	Promoter hypermethylation of SOX11 promotes the progression of cervical cancer in vitro and in vivo
作者	Li, Xiaofang 1,2; Wu, Xiaoling 1; Li, Yun 3; Cui, Yumeng 1; Tian, Run 4; Singh, Namita 1; Ding, Muyang 1; Yang, Yang 1; Gao, Yan'e 1
发表日期	2019-04
发表期刊	ONCOLOGY REPORTS 影响因子和分区
语种	英语
原始文献类型	Article
关键词	SRY-related HMG-box gene 11 cervical cancer CpG island methylation demethylated reagent
其他关键词	SEX-DETERMINING REGION ; TUMOR-SUPPRESSOR GENE ; DNA METHYLATION ; EXPRESSION ; IDENTIFICATION ; EPIGENETICS ; DIAGNOSIS ; PROMISE ; FAMILY ; BREAST
摘要	The development of cervical cancer (CC) is a multi-gene, multi-step carcinogenic process that involves complex genetic and epigenetic mechanisms. SRY-related HMG-box gene 11 (SOX11) is a member of the SOX family of transcription factors with an emerging crucial role in the development of various tumor types. To elucidate the function of SOX11 in cervical carcinogenesis, the expression level of SOX11 during the development of human CC was analyzed by immunohistochemistry and western blot analysis. Additionally, the methylation status of the SOX11 was examined using bisulfite sequencing and methylation-specific polymerase chain reaction. The SOX11 expression and promoter methylation in human CC cell lines were also determined. The effect of SOX11 expression restoration after 5-aza-2 '-deoxycytidine (5-Aza-dC) treatment on the CC cell proliferation ability was evaluated in CC cell lines. SOX11 was highly expressed in normal cervix (NC) and precancerous low-grade squamous intraepithelial lesions, but weakly expressed or virtually absent in precancerous high-grade squamous intraepithelial lesions and CC, which is consistent with the result of the western blot analysis. Hypermethylation of the SOX11 promoter was detected in CC, which was significantly higher than that in NC samples at each CpG site. The expression level of SOX11 in the CC cell lines was downregulated compared with the positive control, Tera-1human teratoma cell line. Upon 5-Aza-dC treatment, SOX11 expression was significantly upregulated in the CC cell lines at the mRNA and protein levels, and cell proliferation was inhibited. The results indicated that the downregulation of SOX11 in CC is due to the hypermethylation of the SOX11 promoter region. Thus, SOX11 methylation may have a role in the growth of CC cells and cervical carcinogenesis.
资助项目	Sci-tech Program Foundation of Shaanxi Province [2017S-013]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81702578]
出版者	SPANDIDOS PUBL LTD
出版地	ATHENS
ISSN	1021-335X
EISSN	1791-2431
卷号	41 期号:4 页码:2351-2360
DOI	10.3892/or.2019.6993
页数	10
WOS类目	Oncology
WOS研究方向	Oncology
WOS记录号	WOS:000461025800025
收录类别	SCIE ; SCOPUS
URL	查看原文
Pubmed记录号	30720133
Scopus记录号	2-s2.0-85062237246
通讯作者地址	[Gao, Yan'e]Xi An Jiao Tong Univ, Affiliated Hosp 2, Dept Obstet & Gynecol, Outpatient Bldg 7th Floor,157 Xiwu Rd, Xian 710004, Shaanxi, Peoples R China.
引用统计	被引频次：5[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/4987
专题	其他_附属慈溪医院（慈溪市人民医院）
通讯作者	Gao, Yan'e
作者单位	1.Xi An Jiao Tong Univ, Affiliated Hosp 2, Dept Obstet & Gynecol, Outpatient Bldg 7th Floor,157 Xiwu Rd, Xian 710004, Shaanxi, Peoples R China; 2.Northwest Womens & Childrens Hosp, ART Ctr, Xian 710004, Shaanxi, Peoples R China; 3.Wenzhou Med Univ, Affiliated Cixi Hosp, Dept Obstet & Gynecol, Cixi 325000, Zhejiang, Peoples R China; 4.Xi An Jiao Tong Univ, Affiliated Hosp 2, Dept Orthoped, Xian 710004, Shaanxi, Peoples R China
推荐引用方式 GB/T 7714	Li, Xiaofang,Wu, Xiaoling,Li, Yun,et al. Promoter hypermethylation of SOX11 promotes the progression of cervical cancer in vitro and in vivo[J]. ONCOLOGY REPORTS,2019,41(4):2351-2360.
APA	Li, Xiaofang., Wu, Xiaoling., Li, Yun., Cui, Yumeng., Tian, Run., ... & Gao, Yan'e. (2019). Promoter hypermethylation of SOX11 promotes the progression of cervical cancer in vitro and in vivo. ONCOLOGY REPORTS, 41(4), 2351-2360.
MLA	Li, Xiaofang,et al."Promoter hypermethylation of SOX11 promotes the progression of cervical cancer in vitro and in vivo".ONCOLOGY REPORTS 41.4(2019):2351-2360.