手足口病患儿甘露醇结合凝集素基因多态性与其蛋白水平的研究

doi:10.11816/cn.ni.2015-141299

科研成果详情

题名	手足口病患儿甘露醇结合凝集素基因多态性与其蛋白水平的研究
其他题名	Study of MBL gene polymorphism and MBL protein level of children with hand-foot-and-mouth disease (HFMD)
作者	陈洁 1; 周爱华2; 陈晓晴1; 李素华1; 徐志伟1; 陈喻 3
发表日期	2015
发表期刊	中华医院感染学杂志影响因子和分区
语种	中文
原始文献类型	学术期刊
关键词	手足口病患儿甘露醇结合凝集素基因蛋白水平
其他关键词	HFMD ; Children patients ; MBL ; Gene ; Protein level
摘要	目的研究不同病情的手足口病(HFMD)患儿甘露醇结合凝集素(MBL)的基因分型及其蛋白表达水平,探析MBL与HFMD的相关性。方法选择2012年7月-2014年7月收治的200例HFMD患儿,根据不同病情分为3组,一般病例组68例、重症病例组66例、危重病例组66例,采用序列分析法检测所有患儿MBL基因及分型,酶联免疫吸附试验(ELISA)测定血浆MBL蛋白水平并进行比较分析。结果 200例患儿标本中检测到肠道病毒132株,其中肠道病毒71型(EV71)96株占72.7%、柯萨奇病毒14株占10.6%,3组患儿均以EV71感染为主,但一般病例组EV71检出率均显著低于重症及危重症组,而重症及危重症组均未检测出CoxA16,差异均有统计学意义(P<0.05);一般病例组HYPA基因检出频率显著高于重症、危重组,重症组显著高于危重组,一般病例组LXPA基因检出频率显著低于重症、危重组,重症组显著低于危重组;一般病例组患儿MBL蛋白水平(624.8±114.6)ng/ml,显著高于重症及危重症组,重症组患儿MBL蛋白水平(542.7±109.4)ng/ml显著高于危重症组(468.6±94.9)ng/ml,差异均有统计学意义(P<0.05)。结论 MBL基因多态性所致MBL蛋白水平下降与手足口病患儿的病情具有相关性,MBL对手足口病患儿感染具有保护作用。
其他摘要	OBJECTIVE To study MBL gene polymorphism and MBL protein level of children with different state of hand‐foot‐and‐mouth disease so as to discuss the relevance of MBL and HFMD.METHODS Totally 200 children with HFMD in our hospital during Jul.1021 to Jul.2014 were chosen and were divided into 3 groups according to different conditions of diseases with 68 cases in ordinary case group,66 cases in severe case group and 66 cases in critical case group.MBL gene and types of all children were detected by series analysis method and plasma MBL protein level was detected by ELISA.The results of the 2 groups were compared and analyzed.RESULTS A total of 132 strains of enterovirus were detected from the 200 samples, including 96 strains of EV71 enterovirus (72.7%) and 14 strains of Coxsackie virus (10.6%).EV71 was the major pathogens infected by three groups of children.However,the detection rate of EV71 in ordinary case group was obviously higher than severe case group and critical case group and the latter two groups were not detected with CoxA 16.The differences were significant (P<0.05).HYPA gene frequency of ordinary case group was obviously higher than severe case group and critical case group,and severe case group was obviously higher than critical group.LXPA gene frequency of ordinary case group was obviously lower than severe case group and critical case group,and severe case group was obviously lower than critical group.MBL plasma level of ordinary case group was (624.8 ± 114.6) ng/ml,which was obvi‐ously higher than severe case group and critical case group,and MBL plasma level of severe case group was (542.7 ± 109.4) ng/ml and was obviously higher than critical group of (468.6 ± 94.9) ng/ml (P<0.05).CONCLUSION MBL plasma level drop caused by MBL gene polymorphism has relevance with condition of disease of children with HFMD.MBL plays a role of protection for infection of children with HFMD.
资助项目	浙江省医药卫生科技基金资助项目（2012KYB159）;温州市科技计划基金资助项目（Y20120155）
ISSN	1005-4529
卷号	25 期号:11 页码:2405-2407
DOI	10.11816/cn.ni.2015-141299
页数	3
收录类别	CNKI ; 万方 ; 维普 ; 北大核心 ; PKU ; CSCD ; ISTIC
学科领域	医药、卫生 ; 基础医学
URL	查看原文
CSCD记录号	CSCD:5499943
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/49320
专题	附属第二医院_儿童感染科附属第一医院_儿科
作者单位	1.温州医科大学附属第二医院儿童感染科; 2.温州医科大学附属第一医院儿科; 3.浙江大学医学院附属第一医院检验科
第一作者单位	附属第二医院_儿童感染科
第一作者的第一单位	附属第二医院_儿童感染科
推荐引用方式 GB/T 7714	陈洁,周爱华,陈晓晴,等. 手足口病患儿甘露醇结合凝集素基因多态性与其蛋白水平的研究[J]. 中华医院感染学杂志,2015,25(11):2405-2407.
APA	陈洁, 周爱华, 陈晓晴, 李素华, 徐志伟, & 陈喻. (2015). 手足口病患儿甘露醇结合凝集素基因多态性与其蛋白水平的研究. 中华医院感染学杂志, 25(11), 2405-2407.
MLA	陈洁,et al."手足口病患儿甘露醇结合凝集素基因多态性与其蛋白水平的研究".中华医院感染学杂志 25.11(2015):2405-2407.

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