Partial Depletion of Regulatory T Cells Enhances Host Inflammatory Response Against Acute Pseudomonas aeruginosa Infection After Sepsis

doi:10.1007/s10753-018-0821-8

科研成果详情

题名	Partial Depletion of Regulatory T Cells Enhances Host Inflammatory Response Against Acute Pseudomonas aeruginosa Infection After Sepsis
作者	Hu, Zhi-qiang 1,2,5; Yao, Yong-ming 4; Chen, Wei 1,2; Bian, Jia-lan 1,2; Zhao, Lin-jun 1,2; Chen, Long-wang 1,2; Hong, Guang-liang1,2; Lu, Zhong-qiu1,2,3; Zhao, Guang-ju1,2
发表日期	2018-10
发表期刊	INFLAMMATION 影响因子和分区
语种	英语
原始文献类型	Article
关键词	sepsis secondary infection nosocomial infection sepsis regulatory T cells CD25
其他关键词	INTENSIVE-CARE UNITS ; IMMUNE DYSFUNCTION ; NOSOCOMIAL INFECTION ; SEPTIC SHOCK ; MICE ; LUNG ; IMMUNOSUPPRESSION ; PROTECTS
摘要	Immune dysfunction contributes to secondary infection and worse outcomes in sepsis. Regulatory T cells (Tregs) have been implicated in sepsis-induced immunosuppression. Nevertheless, the role of Tregs in secondary infection after sepsis remains to be determined. In the present study, a two-hit model which mimics clinical conditions was used and the potential role of Tregs in secondary Pseudomonas aeruginosa infection post-sepsis was investigated. Results showed that mice were susceptible to secondary P. aeruginosa infection 3days, but not 7days, post-cecal ligation and puncture (CLP). The levels of IL-17A, IL-1, and IL-6 remained low in CLP mice after P. aeruginosa infection, while the levels of IL-10 increased significantly. Additionally, increased number of Tregs in both lung and spleen was observed in two-hit mice. Injection with PC61 (anti-CD25) mAb reduced the number of Tregs by 50% in spleen and 60% in lung of septic mice. This partial depletion of Tregs elevated IL-17A, IL-1, and IL-6 production and decreased IL-10 levels in septic mice with P. aeruginosa infection, leading to lower bacterial load, attenuation of lung injury, and improvement of survival. The present findings demonstrate that Tregs play a crucial role in secondary P. aeruginosa infection after sepsis by modulating the inflammatory response.
资助项目	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81401621] Funding Source: Medline; Natural Science Foundation of Zhejiang ProvinceNatural Science Foundation of Zhejiang Province [LY13H150004] Funding Source: Medline; Wenzhou Municipal Science and Technology Project [2015KYB239] Funding Source: Medline; Excellent Young Talents Program of Traditional Chinese Medicine of Zhejiang Province [2013ZQ023] Funding Source: Medline
出版者	SPRINGER/PLENUM PUBLISHERS
出版地	NEW YORK
ISSN	0360-3997
EISSN	1573-2576
卷号	41 期号:5 页码:1780-1790
DOI	10.1007/s10753-018-0821-8
页数	11
WOS类目	Cell Biology ; Immunology
WOS研究方向	Cell Biology ; Immunology
WOS记录号	WOS:000444287600021
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	29956070
SCOPUSEID	2-s2.0-85049146087
通讯作者地址	[Lu, Zhong-qiu]Emergency Department,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325000,China
Scopus学科分类	Immunology and Allergy;Immunology
引用统计	被引频次：11[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/4816
专题	附属第一医院护理学院
通讯作者	Lu, Zhong-qiu
作者单位	1.Emergency Department,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325000,China; 2.Wenzhou Key Laboratory of Emergency,Critical Care,and Disaster Medicine,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325000,China; 3.College of Nursing,Wenzhou Medical University,Wenzhou,325000,China; 4.Burns Institute,First Affiliated Hospital of PLA General Hospital,Beijing,100048,China; 5.Intensive Care Unit,Ningbo First Hospital,Ningbo,315000,China
第一作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
通讯作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Hu, Zhi-qiang,Yao, Yong-ming,Chen, Wei,et al. Partial Depletion of Regulatory T Cells Enhances Host Inflammatory Response Against Acute Pseudomonas aeruginosa Infection After Sepsis[J]. INFLAMMATION,2018,41(5):1780-1790.
APA	Hu, Zhi-qiang., Yao, Yong-ming., Chen, Wei., Bian, Jia-lan., Zhao, Lin-jun., ... & Zhao, Guang-ju. (2018). Partial Depletion of Regulatory T Cells Enhances Host Inflammatory Response Against Acute Pseudomonas aeruginosa Infection After Sepsis. INFLAMMATION, 41(5), 1780-1790.
MLA	Hu, Zhi-qiang,et al."Partial Depletion of Regulatory T Cells Enhances Host Inflammatory Response Against Acute Pseudomonas aeruginosa Infection After Sepsis".INFLAMMATION 41.5(2018):1780-1790.