Developmental co-exposure of TBBPA and titanium dioxide nanoparticle induced behavioral deficits in larval zebrafish

doi:10.1016/j.ecoenv.2021.112176

科研成果详情

题名	Developmental co-exposure of TBBPA and titanium dioxide nanoparticle induced behavioral deficits in larval zebrafish
作者	Chen, Jiangfei1; Li, Jiani 1; Jiang, Hao 1; Yu, Jiajian 1; Wang, Hongzhu 1; Wang, Nengzhuang 1; Chen, Shan2; Mo, Wen 3; Wang, Ping4; Tanguay, Robyn L.5; Dong, Qiaoxiang1,2; Huang, Changjiang 1
发表日期	2021-06-01
发表期刊	ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	TBBPA Behavior Cell apoptosis Oxidative stress Zebrafish
摘要	Both tetrabromobisphenol A (TBBPA) and titanium dioxide nanoparticle (TiO2 NP) have widespread commercial applications, resulting in their ubiquitous co-presence in the environment and biota. Although environmental chemicals exist as mixtures, toxicity studies are nearly always conducted with single chemicals. Few studies explore potential interactions of different chemical mixtures. In this study, we employ the sensitive developing nerve system in zebrafish to assess the neurotoxicity of TBBPA/TiO2 NP mixtures. Specifically, zebrafish embryos were exposed to solvent control (0.1% DMSO), 2 ?M TBBPA, 0.1 mg/L TiO2 NP, and their mixture from 8 to 120 h post fertilization (hpf), and motor/social behavioral assessments were conducted on embryos/larvae at different developmental stages. Our results showed that TBBPA/TiO2 NP single or co-exposures increased spontaneous movement, decreased touch response and swim speed, and affected social behaviors of light/dark preference, shoaling, mirror attack and social contact. In particular, many of these phenotypes were manifested with higher magnitude of changes from the mixture exposure. These behavioral deficits were also accompanied with increased cell death in olfactory region and neuromasts in the lateral line system, increased ROS in gallbladder, pancreas, liver, and intestine, as well as increased lipid peroxidation and decreased ATP levels in whole larval tissue homogenates. Further, genes coding for key cell apoptosis marker and antioxidant enzyme were significantly upregulated by these two chemicals, in particular to their mixture. Interestingly, the co-presence of TBBPA also increased the mean particle size of TiO2 NP in the exposure solutions and the TiO2 NP content in larval tissue. Together, our analysis suggests that TBBPA/TiO2 NP induced behavioral changes may be due to physical accumulation of these two chemicals in the target organs, and TiO2 NP may serve as carriers for increased accumulation of TBBPA. To conclude, we demonstrated that TBBPA/TiO2 NP together cause increased bioaccumulation of TiO2, and heightened responses in behavior, cell apoptosis and oxidative stress. Our findings also highlight the importance of toxicity assessment using chemical mixtures.
资助项目	Natural Science Foundation of Zhejiang ProvinceNatural Science Foundation of Zhejiang Province [LY18B070010]; Public Welfare Program of Wenzhou Science and Technology Bureau [Y20170147]; Talent Scientific Research Projects of Wenzhou Medical University [QTJ16025]
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
出版地	SAN DIEGO
ISSN	0147-6513
EISSN	1090-2414
卷号	215 页码:112176
DOI	10.1016/j.ecoenv.2021.112176
页数	10
WOS类目	Environmental Sciences ; Toxicology
WOS研究方向	Environmental Sciences & Ecology ; Toxicology
WOS记录号	WOS:000637943300004
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	33780780
SCOPUSEID	2-s2.0-85103289544
通讯作者地址	[Chen, Jiangfei]Institute of Environmental Safety and Human Health,School of Public Health and Management,Wenzhou Medical University,Wenzhou,325035,China
Scopus学科分类	Pollution;Public Health, Environmental and Occupational Health;Health, Toxicology and Mutagenesis
TOP期刊	TOP期刊
引用统计	被引频次：4[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/4487
专题	公共卫生学院_健康与环境生态研究所药学院（分析测试中心）附属第二医院第二临床医学院、附属第二医院、育英儿童医院公共卫生学院_环境安全与健康风险评估研究院
通讯作者	Chen, Jiangfei
作者单位	1.Institute of Environmental Safety and Human Health,School of Public Health and Management,Wenzhou Medical University,Wenzhou,325035,China; 2.The Second Affiliated Hospital and Yuying Children's Hospital,Wenzhou Medical University,Wenzhou,325035,China; 3.Zhejiang Rehabilitation Medical Center,Hangzhou,310051,China; 4.School of Pharmaceutical Sciences,Wenzhou Medical University,Wenzhou,325035,China; 5.Sinnhuber Aquatic Research Laboratory,Department of Environmental & Molecular Toxicology,Oregon State University,28645 East Highway 34,Corvallis,97333,United States
第一作者单位	公共卫生学院; 环境安全与健康风险评估研究院
通讯作者单位	公共卫生学院; 环境安全与健康风险评估研究院
第一作者的第一单位	公共卫生学院
推荐引用方式 GB/T 7714	Chen, Jiangfei,Li, Jiani,Jiang, Hao,et al. Developmental co-exposure of TBBPA and titanium dioxide nanoparticle induced behavioral deficits in larval zebrafish[J]. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,2021,215:112176.
APA	Chen, Jiangfei., Li, Jiani., Jiang, Hao., Yu, Jiajian., Wang, Hongzhu., ... & Huang, Changjiang. (2021). Developmental co-exposure of TBBPA and titanium dioxide nanoparticle induced behavioral deficits in larval zebrafish. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, 215, 112176.
MLA	Chen, Jiangfei,et al."Developmental co-exposure of TBBPA and titanium dioxide nanoparticle induced behavioral deficits in larval zebrafish".ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 215(2021):112176.