Co-regulation of LPS and tensile strain downregulating osteogenicity via c-fos expression

doi:10.1016/j.lfs.2013.05.015

科研成果详情

题名	Co-regulation of LPS and tensile strain downregulating osteogenicity via c-fos expression
作者	Wang, Yi1; Wang, Haiyan1; Ye, Qingsong 2; Ye, Jie 1; Xu, Chunyan 3; Lin, Leilei1; Deng, Hui4; Hu, Rongdang1
发表日期	2013-07-19
发表期刊	LIFE SCIENCES 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Alkaline phosphatase Four-point bending device Inflammation Lipopolysaccharide Macrophage Mechanical loading Osteoblasts Tensile strain
其他关键词	PERIODONTAL-LIGAMENT CELLS ; OSTEOBLAST-LIKE CELLS ; TUMOR-NECROSIS-FACTOR ; PORPHYROMONAS-GINGIVALIS ; IN-VITRO ; BONE ; DIFFERENTIATION ; INTERLEUKIN-1 ; FLUID ; MACROPHAGES
摘要	Aims: Orthodontic forces are known to aggravate inflammation-induced destruction of the periodontium, but the underlying mechanism has not been elucidated. The present study investigates how inflammation and forces co-regulate periodontium damage. Main methods: Cultures of MC3T3-E1 osteoblasts were pre-treated with conditioned medium from RAW264.7 macrophages exposed to 100 ng/ml Porphyromonas gingivalis (Pg)-LPS. Conditioned medium was analyzed by ELISA for interleukin-1 beta (IL-1 beta), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-alpha). Osteoblasts were then subjected to tensile strain (0.5 Hz; 1000 mu or 3000 mu) for 0 min, 5 min, 15 min, 30 min, 1 h, 3 h, and 6 h. The cultures were analyzed for mRNA and protein levels of c-fos. Cells were also analyzed for alkaline phosphatase (ALP) activity. Key findings: (Pg)-LPS stimulated the secretion of all three cytokines from RAW264.7 cells in a dose- and time-dependent manner. Medium from (Pg)-LPS stimulated cells induced a 10-fold increase in c-fos expression, which decreased to a 4-fold plateau after 3 h. In contrast, ALP activity of control osteoblasts decreased during the first 60 min, then recovered over the next 4 h. Pretreatment with conditioned medium generated the same initial decrease during tensile strain but prevented the recovery. Significance: Our study showed, for the first time, that the inhibitory effect of inflammation and tensile strain on osteogenicity is associated with the upregulation in c-fos expression. In addition, inflammation may reduce the ability of osteoblasts to restore their osteogenic capacity during sustained tensile stress and contribute to periodontium damage. (C) 2013 Elsevier Inc. All rights reserved.
资助项目	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81200795]; Provincial Nature Science Foundation of Zhejiang [Y207360]
出版者	PERGAMON-ELSEVIER SCIENCE LTD
出版地	OXFORD
ISSN	0024-3205
EISSN	1879-0631
卷号	93 期号:1 页码:38-43
DOI	10.1016/j.lfs.2013.05.015
页数	6
WOS类目	Medicine, Research & Experimental ; Pharmacology & Pharmacy
WOS研究方向	Research & Experimental Medicine ; Pharmacology & Pharmacy
WOS记录号	WOS:000321604900006
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	23727355
SCOPUSEID	2-s2.0-84879498421
通讯作者地址	[Deng, Hui]Periodontal Department,School of Stomatology,Wenzhou Medical College,China
Scopus学科分类	Biochemistry, Genetics and Molecular Biology (all);Pharmacology, Toxicology and Pharmaceutics (all)
TOP期刊	TOP期刊
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/4447
专题	口腔医学院、附属口腔医院_口腔正畸
通讯作者	Deng, Hui
作者单位	1.Orthodontic Department,School of Stomatology,Wenzhou Medical College,China; 2.Department of Orthodontics,James Cook University,Australia; 3.Orthodontic Department,Yiwu Central Hospital,China; 4.Periodontal Department,School of Stomatology,Wenzhou Medical College,China
第一作者单位	口腔医学院、附属口腔医院
通讯作者单位	口腔医学院、附属口腔医院
第一作者的第一单位	口腔医学院、附属口腔医院
推荐引用方式 GB/T 7714	Wang, Yi,Wang, Haiyan,Ye, Qingsong,et al. Co-regulation of LPS and tensile strain downregulating osteogenicity via c-fos expression[J]. LIFE SCIENCES,2013,93(1):38-43.
APA	Wang, Yi., Wang, Haiyan., Ye, Qingsong., Ye, Jie., Xu, Chunyan., ... & Hu, Rongdang. (2013). Co-regulation of LPS and tensile strain downregulating osteogenicity via c-fos expression. LIFE SCIENCES, 93(1), 38-43.
MLA	Wang, Yi,et al."Co-regulation of LPS and tensile strain downregulating osteogenicity via c-fos expression".LIFE SCIENCES 93.1(2013):38-43.