Urolithin A-induced mitophagy suppresses apoptosis and attenuates intervertebral disc degeneration via the AMPK signaling pathway

doi:10.1016/j.freeradbiomed.2020.02.024

科研成果详情

题名	Urolithin A-induced mitophagy suppresses apoptosis and attenuates intervertebral disc degeneration via the AMPK signaling pathway
作者	Lin, Jialiang 1,2,3; Zhuge, Jinru3,4; Zheng, Xuanqi 1,2,3; Wu, Yuhao 3; Zhang, Zengjie 1,2,3; Xu, Tianzhen 1,2,3; Meftah, Zaher 1,2,3; Xu, Hongming5; Wu, Yaosen1,2,3; Tian, Naifeng1,2,3; Gao, Weiyang1,2,3; Zhou, Yifei1,2,3; Zhang, Xiaolei1,2,3,6; Wang, Xiangyang1,2,3
发表日期	2020-04
发表期刊	FREE RADICAL BIOLOGY AND MEDICINE 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Urolithin A Intervertebral disc degeneration Apoptosis Mitophagy AMPK
其他关键词	ENDOPLASMIC-RETICULUM STRESS ; ELLAGIC ACID ; LIPID-PEROXIDATION ; MITOCHONDRIAL ; ELLAGITANNINS ; MECHANISMS ; METABOLITE ; IDENTIFICATION ; ANTIOXIDANT ; ACTIVATION
摘要	Intervertebral disc degeneration (IDD) is a major cause of low back pain (LBP), and effective therapies are still lacking. Previous studies reported that mitochondrial dysfunction contributes to apoptosis, and urolithin A (UA) specifically induces mitophagy. Herein, we aimed to investigate the protective effect of UA-induced mitophagy on tert-butyl hydroperoxide (TBHP)-induced apoptosis in nucleus pulposus (NP) cells in vitro and a rat model of IDD in vivo. Mitochondrial function, apoptosis, and mitophagy were measured in UA-treated NP cells by western blotting and immunofluorescence; the therapeutic effects of UA on IDD were assessed in rats with punctureinduced IDD. The results showed that UA could activate mitophagy in primary NP cells, and UA treatment inhibited TBHP-induced mitochondrial dysfunction and the intrinsic apoptosis pathway. Mechanistically, we revealed that UA promoted mitophagy by activating AMPK signaling in TBHP-induced NP cells. In vivo, UA was shown to effectively alleviate the progression of puncture-induced IDD in rats. Taken together, our results suggest that UA could be a novel and effective therapeutic strategy for IDD.
资助项目	Zhejiang Public Service Technology Research Program/Social Development [LGF18H060008]; Zhejiang Provincial Natural Science Foundation of ChinaNatural Science Foundation of Zhejiang Province [LY17H060010, LY18H060012]; Major Scientific and Technological Project of Medical and Health in Zhejiang Province [WKJ-ZJ-1527]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81601963]; Medical and Health Science and Technology Program of Zhejiang Province [2018KY740]; Wenzhou Science and Technology Bureau Foundation [Y20170092, Y20170083]
出版者	ELSEVIER SCIENCE INC
出版地	NEW YORK
ISSN	0891-5849
EISSN	1873-4596
卷号	150 页码:109-119
DOI	10.1016/j.freeradbiomed.2020.02.024
页数	11
WOS类目	Biochemistry & Molecular Biology ; Endocrinology & Metabolism
WOS研究方向	Biochemistry & Molecular Biology ; Endocrinology & Metabolism
WOS记录号	WOS:000569787100011
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	32105828
SCOPUSEID	2-s2.0-85080057225
通讯作者地址	[Wang, Xiangyang]Department of Orthopaedics,The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University,109 West Xueyuan Road,Wenzhou,325027,China
Scopus学科分类	Biochemistry;Physiology (medical)
TOP期刊	TOP期刊
引用统计	被引频次：48[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/4379
专题	第二临床医学院、附属第二医院、育英儿童医院附属第二医院其他_附属慈溪医院（慈溪市人民医院）
通讯作者	Wang, Xiangyang
作者单位	1.Department of Orthopaedics,The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University,Wenzhou,China; 2.Key Laboratory of Orthopaedics of Zhejiang Province,Wenzhou,China; 3.The Second School of Medicine,Wenzhou Medical University,Wenzhou,China; 4.Department of Anesthesiology,The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University,Wenzhou,China; 5.Department of Orthopaedic Surgery,Affiliated Cixi Hospital of Wenzhou Medical University,Ningbo,China; 6.Chinese Orthopaedic Regenerative Medicine Society,Hangzhou,China
第一作者单位	第二临床医学院，附属第二医院、育英儿童医院; 附属第二医院; 温州医科大学
通讯作者单位	第二临床医学院，附属第二医院、育英儿童医院; 附属第二医院
第一作者的第一单位	第二临床医学院，附属第二医院、育英儿童医院
推荐引用方式 GB/T 7714	Lin, Jialiang,Zhuge, Jinru,Zheng, Xuanqi,et al. Urolithin A-induced mitophagy suppresses apoptosis and attenuates intervertebral disc degeneration via the AMPK signaling pathway[J]. FREE RADICAL BIOLOGY AND MEDICINE,2020,150:109-119.
APA	Lin, Jialiang., Zhuge, Jinru., Zheng, Xuanqi., Wu, Yuhao., Zhang, Zengjie., ... & Wang, Xiangyang. (2020). Urolithin A-induced mitophagy suppresses apoptosis and attenuates intervertebral disc degeneration via the AMPK signaling pathway. FREE RADICAL BIOLOGY AND MEDICINE, 150, 109-119.
MLA	Lin, Jialiang,et al."Urolithin A-induced mitophagy suppresses apoptosis and attenuates intervertebral disc degeneration via the AMPK signaling pathway".FREE RADICAL BIOLOGY AND MEDICINE 150(2020):109-119.