苏拉明通过抑制纤维增生和炎症反应减轻小鼠的瘢痕增生

doi:10.3969/j.issn.1000-4718.2019.11.022

科研成果详情

题名	苏拉明通过抑制纤维增生和炎症反应减轻小鼠的瘢痕增生
其他题名	Suramin alleviates hypertrophic scar in mice by inhibiting fibroplasia and inflammatory response
作者	林康1; 苏海燕2; 虞庆1; 蒋良福1; 高伟阳1
发表日期	2019-11-15
发表期刊	中国病理生理杂志影响因子和分区
语种	中文
原始文献类型	学术期刊
关键词	苏拉明纤维增生炎症反应增生性瘢痕
摘要	目的:探讨苏拉明对增生性瘢痕(HPS)的治疗作用及其机制。方法:使用机械牵拉的方法制造小鼠HPS模型后,分别在瘢痕部位涂抹低剂量(5 mg/kg)和高剂量(10 mg/kg)苏拉明溶液,每天1次,持续10 d。宏观观察瘢痕增生的程度;苏木精-伊红(HE)染色评价HPS组织中瘢痕横截面积与瘢痕抬高指数;然后通过免疫组化法检测HPS组织中转化生长因子β1(TGF-β1)和白细胞介素6(IL-6)的表达水平;免疫荧光、RT-qPCR和Wes-tern blot法分别检测HPS组织中α-平滑肌肌动蛋白(α-SMA)的表达水平; ELISA检测HPS组织中肿瘤坏死因子α(TNF-α)、IL-6、IL-10和TGF-β1的含量。结果:大体观察可见,低、高剂量苏拉明治疗后HPS小鼠瘢痕表面积均显著减少(P<0.01);HE染色下可见低、高剂量苏拉明治疗后HPS小鼠瘢痕横截面积和瘢痕抬高指数均显著减少(P<0.05或P<0.01);免疫荧光染色、RT-qPCR和Western blot结果显示,低、高剂量苏拉明治疗后HPS小鼠瘢痕组织中α-SMA阳性细胞数及α-SMA mRNA表达和蛋白表达均显著降低(P<0.05或P<0.01);免疫组化结果显示,低、高剂量苏拉明治疗后HPS小鼠瘢痕组织中TGF-β1和IL-6表达均显著降低(P<0.01);ELISA结果显示,低、高剂量苏拉明治疗后HPS小鼠瘢痕组织中TNF-α、IL-6、IL-10和TGF-β1水平均显著降低(P<0.01)。结论:苏拉明具有抑制HPS形成的作用,这一作用可能与其抑制纤维增生和减轻局部炎症反应有关。
其他摘要	AIM: To investigate the therapeutical effect of suramin on hypertrophic scar(HPS) and its mechanism. METHODS: After the mouse model of HPS was established by mechanical stretching,the suramin solution at low dose(5 mg/kg) and high dose(10 mg/kg) was applied onto the scar site caused by mechanical load in mice by transdermal administration once a day for 10 d. The degree of scar hyperplasia was observed by macroscopy. The scar cross-sectional area and scar elevation index in the HPS tissues were evaluated by hematoxylin-eosin(HE) staining. The expression levels of transforming growth factor-β1(TGF-β1) and interleukin-6(IL-6) in HPS tissues were detected by immumohistochemical staining. The expression level of α-smooth muscle actin(α-SMA) in HPS tissues was detected by immunofluorescence staining,RT-qPCR and Western blot. The levels of tumor necrosis factor-α(TNF-α), IL-6,IL-10 and TGF-β1 in the HPS tissues were measured by ELISA. RESULTS: Macroscopic observation showed that the surface areas of scar in the HPS mice after treatment with suramin at low and high doses were significantly reduced(P < 0.01). HE staining results showed that the scar cross-sectional area and the scar elevation index of HPS mice after treatment with suramin at low and high doses were significantly reduced(P < 0.05 or P < 0.01). The results of immunofluorescence staining,RT-qPCR and Western blot showed that the number of α-SMA positive cells and the mRNA and protein expression of α-SMA in scar tissues of HPS mice after treatment with suramin at low and high doses were significantly decreased(P < 0.05 or P < 0.01). The results of immunohistochemical staining showed that the expression levels of TGF-β1 and IL-6 in scar tissues of HPS mice after treatment with suramin at low and high doses were significantly reduced(P < 0.01). The results of ELISA showed that the levels of TNF-α,IL-6,IL-10 and TGF-β1 in the scar tissues of HPS mice after treatment with suramin at low and high doses were significantly reduced(P < 0.01). CONCLUSION: Suramin inhibits the formation of HPS,which may be related to the inhibition of fibroplasia and reduction of local inflammatory response.
资助项目	温州市公益性科技计划项目（No.Y20150240）
ISSN	1000-4718
卷号	35 期号:11 页码:2070-2077
DOI	10.3969/j.issn.1000-4718.2019.11.022
页数	8
收录类别	CNKI ; 万方 ; SCOPUS ; 维普 ; 北大核心 ; PKU ; CSCD ; ISTIC
学科领域	医药、卫生 ; 基础医学
URL	查看原文
CSCD记录号	CSCD:6611859
SCOPUSEID	2-s2.0-85171525559
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/43752
专题	附属第二医院附属第二医院_儿童内分泌科
作者单位	1.温州医科大学附属第二医院手外整形科; 2.温州医科大学附属第二医院儿童内分泌科
第一作者单位	附属第二医院
第一作者的第一单位	附属第二医院
推荐引用方式 GB/T 7714	林康,苏海燕,虞庆,等. 苏拉明通过抑制纤维增生和炎症反应减轻小鼠的瘢痕增生[J]. 中国病理生理杂志,2019,35(11):2070-2077.
APA	林康, 苏海燕, 虞庆, 蒋良福, & 高伟阳. (2019). 苏拉明通过抑制纤维增生和炎症反应减轻小鼠的瘢痕增生. 中国病理生理杂志, 35(11), 2070-2077.
MLA	林康,et al."苏拉明通过抑制纤维增生和炎症反应减轻小鼠的瘢痕增生".中国病理生理杂志 35.11(2019):2070-2077.

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