脂肪源性干细胞对多发性硬化患者Th17的免疫调控作用

doi:10.3969/j.issn.1000-4718.2016.01.009

科研成果详情

题名	脂肪源性干细胞对多发性硬化患者Th17的免疫调控作用
其他题名	Adipose-derived stem cells mediate immunosuppression of Th17 in multiple sclerosis
作者	林晓滨 1; 陈颖 2; 杨德壕2; 谢甬淋 3; 毕涌2; 柯建明 2; 陈志博2; 苏中钱 2; 厉向2; 张旭2
发表日期	2016
发表期刊	中国病理生理杂志影响因子和分区
语种	中文
原始文献类型	学术期刊
关键词	多发性硬化脂肪源性干细胞 Th17细胞白血病抑制因子白细胞介素6
其他关键词	Multiple sclerosis ; Adipose-derived stem cells ; Th17 cells ; Leukemia inhibitory factor ; Interleukin-6
摘要	目的:探讨人脂肪源性干细胞(h ASCs)对多发性硬化(MS)患者外周血辅助性T细胞17(Th17)的免疫调控作用机制。方法:分离、纯化脂肪组织中的h ASCs。采用密度梯度离心法分离MS患者外周血单个核细胞(PBMCs),磁珠分选CD4+T细胞,体外刺激细胞向Th17极化,并加入不同比例的h ASCs(h ASCs∶CD4+T为1∶4和1∶10)共培养4 d,设立添加anti-LIF抗体组;流式细胞术检测共培养后Th17细胞占CD4+T细胞的比例,real-time PCR检测白细胞介素6受体(IL-6R)、白细胞介素23受体(IL-23R)、白血病抑制因子受体(LIFR)、维甲酸相关孤儿受体γt(RORγt)及白血病抑制因子(LIF)的mRNA水平变化;ELISA法检测共培养体系上清液中LIF的水平。结果:分离的h ASCs经流式细胞术鉴定可基本判定为h ASCs;PBMCs经磁珠法分选后获得90%以上纯度的CD4+T细胞。共培养后,1∶4组和1∶10组中Th17细胞所占比例下降,且存在高浓度抑制效应;共培养后RORγt、IL-6R和IL-23R的mRNA表达水平下降,LIFR和LIF的mRNA表达水平均升高;加入anti-LIF抗体后,Th17细胞比例回升至对照组水平;RORγt和IL-6R的mRNA表达水平回升;ELISA检测各组LIF的水平,共培养组LIF分泌均较对照组明显增多,加入anti-LIF抗体后明显减少。结论:h ASCs可抑制MS患者Th17细胞的分化,其作用可能与其分泌LIF、通过IL-6/LIF轴竞争性抑制有关。
其他摘要	AIM: To investigate how human adipose-derived stem cells (hASCs) regulates the differentiation of Th17 cells in multiple sclerosis. METHODS: hASCs were isolated from the adipose tissues. Magnetic-activated cell sorting (MACS) kit was used to isolate CD4~+ T cells from peripheral blood mononuclear cells (PBMCs) which were isolated by density gradient centrifugation. The percentage of CD4~+ T cells was detected by flow cytometry. The activated CD4~+ T cells were co-cultured with hASCs for about 4 d at different ratios of hASCs to CD4~+ T cells (1∶ 4 and 1∶ 10) in a Th17 polarised condition. Another group adding anti-leukemia inhibitory factor (LIF) antibody was set up. Th17 cell proportion of the CD4~+ T cells was determined by flow cytometry. The level of LIF in the supernatant of co-cultured system was measured by ELISA. The mRNA expression of retinoid-related orphan receptor γt (RORγt),interleukin-6 receptor (IL-6R), interleukin-23 receptor (IL-23R),LIF and leukemia inhibitory factor receptor (LIFR) was detected by real-time PCR. RESULTS: The result of flow cytometry suggested there were mainly hASCs,and the percentage of CD4~+ T cells in the PBMCs were above 90% after MACS. The Th17 cell proportion decreased in 1∶ 4 and 1∶ 10 co-cultured groups in a dosedependent manner. The mRNA expression of IL-6R,IL-23R and RORγt was downregulated and the expression of LIFR and LIF was up-regulated. When the anti-LIF was added into the co-cultured system,the ratio of Th17 cells increased and reached to the control level. The protein level of LIF obviously increased after co-cultured. After anti-LIF added,the mRNA expression of RORγt and IL-6R was up-regulated. CONCLUSION: hASCs inhibits the differentiation of Th17 cells from multiple sclerosis patients through the competitive inhibition of LIF/IL-6 by secreting LIF.
资助项目	浙江省自然科学基金资助项目（No.LY14H130002,No.LY13H090010）;温州市科技计划（No.Y20140278）
ISSN	1000-4718
卷号	32 期号:01 页码:51-57
DOI	10.3969/j.issn.1000-4718.2016.01.009
页数	7
收录类别	CNKI ; 万方 ; 维普 ; 北大核心 ; PKU ; CSCD ; ISTIC
学科领域	医药、卫生 ; 神经病学与精神病学
URL	查看原文
CSCD记录号	CSCD:5623150
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/43352
专题	附属第一医院附属第一医院_超声影像科第一临床医学院（信息与工程学院）、附属第一医院_内科学_神经内科
作者单位	1.温州医科大学附属第一医院超声影像科; 2.温州医科大学附属第一医院神经内科; 3.武警浙江省总队杭州医院康复科
第一作者单位	超声影像科; 附属第一医院
第一作者的第一单位	超声影像科
推荐引用方式 GB/T 7714	林晓滨,陈颖,杨德壕,等. 脂肪源性干细胞对多发性硬化患者Th17的免疫调控作用[J]. 中国病理生理杂志,2016,32(01):51-57.
APA	林晓滨., 陈颖., 杨德壕., 谢甬淋., 毕涌., ... & 张旭. (2016). 脂肪源性干细胞对多发性硬化患者Th17的免疫调控作用. 中国病理生理杂志, 32(01), 51-57.
MLA	林晓滨,et al."脂肪源性干细胞对多发性硬化患者Th17的免疫调控作用".中国病理生理杂志 32.01(2016):51-57.

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