Urinary miR-21 as a potential biomarker of hypertensive kidney injury and fibrosis

doi:10.1038/s41598-017-18175-3

科研成果详情

题名	Urinary miR-21 as a potential biomarker of hypertensive kidney injury and fibrosis
作者	Chen, Congcong 1,2; Lu, Chaosheng1; Qian, Yan1; Li, Haiyan 1; Tan, Yi1,2,3; Cai, Lu1,2,3; Weng, Huachun1
发表日期	2017-12-18
发表期刊	SCIENTIFIC REPORTS 影响因子和分区
语种	英语
原始文献类型	Article
其他关键词	PROMOTES RENAL FIBROSIS ; DIABETIC-NEPHROPATHY ; MICRORNA EXPRESSION ; MICROARRAY ANALYSIS ; DISEASE ; CANCER ; BETA ; TRANSITION
摘要	Kidney biopsy is considered the golden criterion for diagnosing the etiology of kidney disease but accompanied by non-negligible complications. We explored the possibility of using urinary microRNA (miRNA) as a non-invasive biomarker for hypertensive kidney injury. We assessed differential miRNA expressions in the kidneys and urine of hypertensive mice with kidney injury induced by deoxycorticosterone acetate (DOCA)-salt compared to the controls. DOCA-salt treatment significantly increased renal tubular lesions from day 2 and mRNA expression of fibrosis-related genes from day 4 compared to the controls, respectively. Urinary albumin and N-acetyl-beta-D-glucosaminidase was significantly increased on day 8 compared to the controls. Array results showed that 20 out of 585 miRNAs were highly expressed in the kidneys and significantly increased on day 8 compared to the controls, including miR-21, miR-146b, miR-155 and miR-132, which were confirmed by real-time polymerase chain reaction and were significantly higher from day 4. The miR-21/creatinine in the urine from day 4 was significantly higher than that of the controls and was detected earlier than urinary albumin. In conclusion, we have identified urinary miR-21 that correlates with histopathological lesions and functional markers of kidney damage to facilitate a potential noninvasive detection for hypertensive kidney injury.
资助项目	Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81670784]; Public Welfare Science and Technology project from Wenzhou City [Y20160011]
出版者	NATURE PUBLISHING GROUP
出版地	LONDON
ISSN	2045-2322
卷号	7 期号:1 页码:17737
DOI	10.1038/s41598-017-18175-3
页数	9
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
WOS记录号	WOS:000418323900049
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	29255279
PMC记录号	PMC5735153
SCOPUSEID	2-s2.0-85038561349
通讯作者地址	[Cai, Lu]Chinese-American Research Institute for Pediatrics,Department of Pediatrics,First Affiliated Hospital of Wenzhou Medical University,Wenzhou, Zhejiang,China
Scopus学科分类	Multidisciplinary
引用统计	被引频次：38[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/4262
专题	附属第一医院_儿科药学院（分析测试中心）_药学系
通讯作者	Cai, Lu
作者单位	1.Chinese-American Research Institute for Pediatrics,Department of Pediatrics,First Affiliated Hospital of Wenzhou Medical University,Wenzhou, Zhejiang,China; 2.Department of Pharmaceutical Sciences,Wenzhou Medical University,Chashan University-town,Wenzhou, Zhejiang,China; 3.Pediatric Research Institute,Departments of Pediatrics,Radiation Oncology,Pharmacology and Toxicology,University of Louisville,Louisville, Kentucky,United States
第一作者单位	附属第一医院; 温州医科大学
通讯作者单位	附属第一医院
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Chen, Congcong,Lu, Chaosheng,Qian, Yan,et al. Urinary miR-21 as a potential biomarker of hypertensive kidney injury and fibrosis[J]. SCIENTIFIC REPORTS,2017,7(1):17737.
APA	Chen, Congcong., Lu, Chaosheng., Qian, Yan., Li, Haiyan., Tan, Yi., ... & Weng, Huachun. (2017). Urinary miR-21 as a potential biomarker of hypertensive kidney injury and fibrosis. SCIENTIFIC REPORTS, 7(1), 17737.
MLA	Chen, Congcong,et al."Urinary miR-21 as a potential biomarker of hypertensive kidney injury and fibrosis".SCIENTIFIC REPORTS 7.1(2017):17737.