超声微泡靶向介导MaFGF对阿霉素心肌病防治作用的初步实验研究

doi:10.3760/cma.j.issn.1004-4477.2017.01.016

科研成果详情

题名	超声微泡靶向介导MaFGF对阿霉素心肌病防治作用的初步实验研究
其他题名	Prevention of doxorubicin induced cardiomyopathy after delivery of modified acidic fibroblast growth factor by using ultrasound-targeted microbubble destruction:a preliminary experimental study
作者	倪贤伟1; 田新桥 2; 赵应征3; 郑磊4; 李剑敏5; 吕夕明1; 何慧疗1; 毛凯丽 3; 田福荣 3; 郑靖宇5
发表日期	2017-01-25
发表期刊	中华超声影像学杂志影响因子和分区
语种	中文
原始文献类型	学术期刊
关键词	超声心动描记术微气泡酸性成纤维细胞生长因子心肌病阿霉素
其他关键词	Echocardiography ; Microbubbles ; Acidic fibroblast growth factor ; Cardiomyopathy ; Doxorubicin
摘要	目的探讨应用超声靶向微泡击破技术(ultrasound-targeted microbubble destruction,UTMD)介导改构型酸性成纤维细胞生长因子(modified acidic fibroblast growth factor,MaFGF)对大鼠阿霉素心肌病(doxorubicin-induced cardiomyopathy,DOX-CM)的防治作用。方法 50只健康雄性SD大鼠随机平分为5组:①正常对照组;②DOX-CM模型组;③单纯MaFGF组;④UTMD组;⑤MaFGF+UTMD组。②~⑤组大鼠经腹腔注射盐酸阿霉索(DOX)并给予对应干预。干预6周后对所有大鼠行常规超声心动图及速度向量成像(VVI)检查,测定左室舒张末期内径(LVIDd)、左室收缩末期内径(LVIDs)、左室射血分数(LVEF)、左室短轴缩短率(LVFS)、乳头肌水平左室壁各节段平均径向峰值速度(Vs)、径向应变(Sr)及径向应变率(SRr)。测量结束后处死大鼠取心肌组织,HE染色观察其组织形态学变化,苦味酸天狼猩红(VG)染色计算心肌胶原容积分数(CVF)。结果干预6周后,MaFGF+UTMD组心脏/体重比值(HW/BW)、LVIDs及LVIDd较DOX-CM模型组明显下降(均P<0.05),LVEF、LVFS、Vs、Sc及Sr明显增高(均P<0.05);VG染色显示与正常对照组相比DOX-CM模型组胶原容积分数(CVF)明显增高(P<0.01),而MaFGF+UTMD组CVF降低(P<0.01)。结论应用UTMD介导MaFGF可减缓DOX-CM大鼠心肌纤维化形成,有效保护DOXCM大鼠的左心室收缩功能。
其他摘要	Objective To observe the preventive effects of doxorubicin-induced cardiomyopathy (DOX-CM) rats after delivery of modified acidic fibroblast growth factor (MaFGF) by using ultrasound-targeted microbubble destruction (UTMD). Methods Fifty male SD rats were randomly divided into five groups:①Control group;②DOX-CM group;③MaFGF group;④UTMD group;⑤MaFGF+UTMD group. ②~⑤groups were intraperitoneally injected with doxorubicin to induce DOX-CM and given the corresponding interventions. Six weeks after intervention,all rats were underwent conventional echocardiography and velocity vector imaging (VVI) exams. Left ventricular internal dimension-diastole (LVIDd),left ventricular internal dimension-systole (LVIDs),left ventricular ejection fraction (LVEF) and left ventricular fractional shortening (LVFS) were measured using conventional echocardiography. The segmental mean peak systolic radial velocity (Vs),systolic radial strain (Sr),and systolic radial strain rate (SRr) of six walls at the papillary muscle level were measured in left ventricular short-axis view by VVI. At last,myocardial tissues of all rats were stained with HE to observe the myocardial tissue morphology and with Van Gieson (VG) to calculate the collagen volume fractions (CVF). Results Six weeks after intervention,heart weight/body weight (HW/BW),LVIDs and LVIDd of MaFGF+UTMD group were significantly decreased compared with those of DOX-CM group(P<0.05),while the LVEF,LVFS,Vs,Sc and Sr were significantly increased (P<0.05). Van Gieson stains showed that the collagen volume fractions (CVF) of DOX-CM groups significantly increased compared with that of the control group (P<0.01),while the CVF of MaFGF+UTMD groups significantly decreased (P<0.01). Conclusions Delivery of MaFGF to myocardium in DOX-CM rats by UTMD could retard the formation of myocardial fibrosis and effectively protect the left ventricular function of DOX-CM rats.
资助项目	国家自然科学基金（81571696,81301982）;温州市公益性科技计划项目（Y20140726）~~
ISSN	1004-4477
卷号	26 期号:01 页码:71-76
DOI	10.3760/cma.j.issn.1004-4477.2017.01.016
页数	6
收录类别	CNKI ; 万方 ; 维普 ; 北大核心 ; PKU ; CSCD ; ISTIC
学科领域	医药、卫生
URL	查看原文
CSCD记录号	CSCD:5925341
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/40803
专题	第二临床医学院，附属第二医院、育英儿童医院_影像医学与核医学_超声科温州医科大学
作者单位	1.温州医科大学附属第二医院超声科; 2.河南省人民医院(郑州大学人民医院)超声科; 3.温州医科大学; 4.温州医科大学附属第一医院超声科; 5.温州医科大学附属第一医院病理科
第一作者单位	第二临床医学院，附属第二医院、育英儿童医院_影像医学与核医学_超声科
第一作者的第一单位	第二临床医学院，附属第二医院、育英儿童医院_影像医学与核医学_超声科
推荐引用方式 GB/T 7714	倪贤伟,田新桥,赵应征,等. 超声微泡靶向介导MaFGF对阿霉素心肌病防治作用的初步实验研究[J]. 中华超声影像学杂志,2017,26(01):71-76.
APA	倪贤伟., 田新桥., 赵应征., 郑磊., 李剑敏., ... & 郑靖宇. (2017). 超声微泡靶向介导MaFGF对阿霉素心肌病防治作用的初步实验研究. 中华超声影像学杂志, 26(01), 71-76.
MLA	倪贤伟,et al."超声微泡靶向介导MaFGF对阿霉素心肌病防治作用的初步实验研究".中华超声影像学杂志 26.01(2017):71-76.