Mining, Validation, and Clinical Significance of Colorectal Cancer (CRC)-Associated lncRNAs

doi:10.1371/journal.pone.0164590

科研成果详情

题名	Mining, Validation, and Clinical Significance of Colorectal Cancer (CRC)-Associated lncRNAs
作者	Sun, Xiangwei1; Hu, Yingying2; Zhang, Liang 1; Hu, Changyuan1; Guo, Gangqiang3; Mao, Chenchen 1; Xu, Jianfeng 1; Ye, Sisi 3; Huang, Guanli4; Xue, Xiangyang3; Guo, Aizhen 5; Shen, Xian1,6
发表日期	2016-10-27
发表期刊	PLOS ONE 影响因子和分区
语种	英语
原始文献类型	Article
其他关键词	LONG NONCODING RNA ; POOR-PROGNOSIS ; GASTRIC-CANCER ; CELL-PROLIFERATION ; COLON-CANCER ; METASTASIS ; EXPRESSION ; METAANALYSIS ; MIGRATION ; PROSTATE
摘要	Background Colorectal cancer (CRC) is one of the deadliest tumours, but its pathogenesis remains unclear. The involvement of differentially expressed long non-coding RNAs (lncRNAs) in CRC tumorigenesis makes them suitable tumour biomarkers. Methods/Findings Here, we screened 150 cases of CRC and 85 cases of paracancerous tissues in the GEO database for differentially expressed lncRNAs. The levels of lncRNA candidates in 84 CRC and paracancerous tissue samples were validated by qRT-PCR and their clinical significance was analyzed. We identified 15 lncRNAs with differential expression in CRC tumours; among them, AK098081 was significantly up-regulated, whereas AK025209, BC040303, BC037331, AK026659, and CR749831 were down-regulated in CRC. In a receiver operating characteristic curve analysis, the area under the curve for the six lncRNAs was 0.914. High expression of AK098081 and low expression of BC040303, CR749831, and BC037331 indicated poor CRC differentiation. CRC patients with lymph node metastasis had lower expression of BC037331. In addition, the group with high AK098081 expression presented significantly lower overall survival and disease-free survival rates than the low-expression group, confirming AK098081 as an independent risk factor for CRC patients. Conclusion/Significance In conclusion, we have identified multiple CRC-associated lncRNAs from microarray expression profiles that can serve as novel biomarkers for the diagnosis and prognosis of CRC.
资助项目	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81001343, 81472308, 31470891]; Wenzhou Municipal Science and Technology Bureau [Y20150050, Y20150054, Y20150057]
出版者	PUBLIC LIBRARY SCIENCE
出版地	SAN FRANCISCO
ISSN	1932-6203
卷号	11 期号:10 页码:e0164590.
DOI	10.1371/journal.pone.0164590
页数	14
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
WOS记录号	WOS:000389604900023
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	27788162
PMC记录号	PMC5082825
SCOPUSEID	2-s2.0-84992688656
通讯作者地址	[Shen, Xian]Wenzhou Med Univ, Affiliated Hosp 1, Dept Gen Surg, Wenzhou, Peoples R China. ; [Xue, Xiangyang]Wenzhou Med Univ, Dept Microbiol & Immunol, Inst Mol Virol & Immunol, Inst Trop Med, Wenzhou, Peoples R China. ; [Guo, Aizhen]Tongji Univ, Sch Med, Yangpu Hosptial, Dept Internal Med, Shanghai, Peoples R China. ; [Shen, Xian]Wenzhou Med Univ, Affiliated Hosp 2, Dept Gen Surg, Wenzhou, Peoples R China.
Scopus学科分类	Multidisciplinary
引用统计	被引频次：13[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/4002
专题	附属第一医院附属第二医院第二临床医学院、附属第二医院、育英儿童医院检验医学院（生命科学学院、生物学实验教学中心）_病原生物学与免疫学系
通讯作者	Xue, Xiangyang; Guo, Aizhen; Shen, Xian
作者单位	1.Wenzhou Med Univ, Affiliated Hosp 1, Dept Gen Surg, Wenzhou, Peoples R China; 2.Wenzhou Med Univ, Affiliated Hosp 2, Dept Obstet & Gynecol, Wenzhou, Peoples R China; 3.Wenzhou Med Univ, Dept Microbiol & Immunol, Inst Mol Virol & Immunol, Inst Trop Med, Wenzhou, Peoples R China; 4.Wenzhou Med Univ, Affiliated Hosp 1, Dept Surg Oncol, Wenzhou, Peoples R China; 5.Tongji Univ, Sch Med, Yangpu Hosptial, Dept Internal Med, Shanghai, Peoples R China; 6.Wenzhou Med Univ, Affiliated Hosp 2, Dept Gen Surg, Wenzhou, Peoples R China
第一作者单位	附属第一医院
通讯作者单位	附属第一医院; 温州医科大学; 附属第二医院; 第二临床医学院，附属第二医院、育英儿童医院
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Sun, Xiangwei,Hu, Yingying,Zhang, Liang,et al. Mining, Validation, and Clinical Significance of Colorectal Cancer (CRC)-Associated lncRNAs[J]. PLOS ONE,2016,11(10):e0164590..
APA	Sun, Xiangwei., Hu, Yingying., Zhang, Liang., Hu, Changyuan., Guo, Gangqiang., ... & Shen, Xian. (2016). Mining, Validation, and Clinical Significance of Colorectal Cancer (CRC)-Associated lncRNAs. PLOS ONE, 11(10), e0164590..
MLA	Sun, Xiangwei,et al."Mining, Validation, and Clinical Significance of Colorectal Cancer (CRC)-Associated lncRNAs".PLOS ONE 11.10(2016):e0164590..