Determination of lesinurad in rat plasma by a UHPLC-MS/MS assay

doi:10.1186/s13065-017-0353-6

科研成果详情

题名	Determination of lesinurad in rat plasma by a UHPLC-MS/MS assay
作者	Zhou, Xiao-Yang 1; Yuan, Ling-Jing 2; Chen, Zhe 2; Tang, Peng-Fei 2; Li, Xiang-Yu 2; Hu, Guo-Xin2; Cai, Jian-Ping 1
发表日期	2017-11-28
发表期刊	CHEMISTRY CENTRAL JOURNAL 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Lesinurad UHPLC-MS/MS Rat plasma Pharmacokinetics
其他关键词	URIC-ACID ; GOUT ; PHARMACODYNAMICS ; PHARMACOKINETICS ; HYPERURICEMIA
摘要	Lesinurad is an oral inhibitor of urate-anion exchanger transporter 1 and has been approved by the US Food and Drug Administration for combination therapy with a xanthine oxidase inhibitor for the treatment of hyperuricemia associated with refractory gout. In the present study, a sensitive and specific ultra high-performance liquid chromatography with tandem mass spectrometry assay was established and verified for the determination of lesinurad in rat plasma and was described in details for the first time. Chromatographic separation of lesinurad and diazepam (internal standard, IS) was performed on a Rapid Resolution HT C18 column (3.0 x 100 mm, 1.8 mu m) using methanol-water (70:30, v/v) as the mobile phase at a flow rate of 0.3 mL/min. Lesinurad and IS were extracted from plasma by liquid-liquid extraction using ethyl acetate. The mass spectrometric detection was carried out using an electrospray ionization source in positive mode. Multiple reaction monitoring was used for quantification of the precursor to product ion at m/z 405.6 -> 220.9 for lesinurad and m/z 285.1 -> 192.8 for IS. The assay was well validated for selectivity, accuracy, precision, recovery, linearity, matrix effects, and stability. The verified method was applied to obtain the pharmacokinetic parameters and concentration-time profiles for lesinurad after oral/intravenous administration in rats. The study might provide an important reference and a necessary complement for the qualitative and quantitative evaluation of lesinurad.
资助项目	Ministry of Science and Technology of the People's Republic of ChinaMinistry of Science and Technology, China [2017ZX09304026]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31371280]
出版者	SPRINGEROPEN
出版地	LONDON
ISSN	1752-153X
卷号	11 期号:1 页码:1-7
DOI	10.1186/s13065-017-0353-6
页数	7
WOS类目	Chemistry, Multidisciplinary
WOS研究方向	Chemistry
WOS记录号	WOS:000416335200001
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	29181594
PMC记录号	PMC5704027
SCOPUSEID	2-s2.0-85035132706
通讯作者地址	[Cai, Jian-Ping]Beijing Hospital,National Center of Gerontology,The MOH Key Laboratory of Geriatrics,Beijing,100730,China
Scopus学科分类	Chemistry (all)
引用统计	被引频次：11[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/3992
专题	药学院（分析测试中心）药学院（分析测试中心）_临床药学与药理系
通讯作者	Cai, Jian-Ping
作者单位	1.Beijing Hospital,National Center of Gerontology,The MOH Key Laboratory of Geriatrics,Beijing,100730,China; 2.Wenzhou Medical University,Department of Pharmacology,School of Pharmacy,Wenzhou, Zhejiang,325035,China
推荐引用方式 GB/T 7714	Zhou, Xiao-Yang,Yuan, Ling-Jing,Chen, Zhe,et al. Determination of lesinurad in rat plasma by a UHPLC-MS/MS assay[J]. CHEMISTRY CENTRAL JOURNAL,2017,11(1):1-7.
APA	Zhou, Xiao-Yang., Yuan, Ling-Jing., Chen, Zhe., Tang, Peng-Fei., Li, Xiang-Yu., ... & Cai, Jian-Ping. (2017). Determination of lesinurad in rat plasma by a UHPLC-MS/MS assay. CHEMISTRY CENTRAL JOURNAL, 11(1), 1-7.
MLA	Zhou, Xiao-Yang,et al."Determination of lesinurad in rat plasma by a UHPLC-MS/MS assay".CHEMISTRY CENTRAL JOURNAL 11.1(2017):1-7.