Effect of puerarin in promoting fatty acid oxidation by increasing mitochondrial oxidative capacity and biogenesis in skeletal muscle in diabetic rats

doi:10.1038/s41387-017-0009-6

科研成果详情

题名	Effect of puerarin in promoting fatty acid oxidation by increasing mitochondrial oxidative capacity and biogenesis in skeletal muscle in diabetic rats
作者	Chen, Xiu-fang1,2; Wang, Lei 1; Wu, Yong-zheng 1; Song, Shi-yu 1; Min, Hai-yan 1; Yang, Yan 3; He, Xuan 1; Liang, Qiao 1; Yi, Long 1; Wang, Yong 1; Gao, Qian 1
发表日期	2018-06-12
发表期刊	NUTRITION & DIABETES 影响因子和分区
语种	英语
原始文献类型	Article
其他关键词	ACTIVATED PROTEIN-KINASE ; INSULIN-RESISTANCE ; LIPID-METABOLISM ; STRESS ; PHOSPHORYLATION ; GLUCOSE ; DYSFUNCTION ; PGC-1-ALPHA ; EXPRESSION ; PALMITATE
摘要	Background: Type 2 diabetes is characterized by dyslipidemia and the accumulation of lipids in non-adipose tissue, including skeletal muscle. Puerarin, which is a natural isoflavonoid isolated from the root of the plant Pueraria lobata, has been shown to have antidiabetic activity. However, the lipid-reducing effect of puerarin, in particular in skeletal muscle, has not yet been addressed. Methods: We examined the effect of puerarin on mitochondrial function and the oxidation of fatty acids in the skeletal muscle of high-fat diet/streptozotocin-induced diabetic rats. Results: Puerarin effectively alleviated dyslipidemia and decreased the accumulation of intramyocellular lipids by upregulating the expression of a range of genes involved in mitochondrial biogenesis, oxidative phosphorylation, the detoxification of reactive oxygen species, and the oxidation of fatty acids in the muscle of diabetic rats. Also, the effect of puerarin on mitochondrial biogenesis might partially involve the function of the mu-opioid receptor. In addition, puerarin decreased the trafficking of fatty acid translocase/CD36 to the plasma membrane to reduce the uptake of fatty acids by myocytes. In vitro studies confirmed that puerarin acted directly on muscle cells to promote the oxidation of fatty acids in insulin-resistant myotubes treated with palmitate. Conclusions: Puerarin improved the performance of mitochondria in muscle and promoted the oxidation of fatty acids, which thus prevented the accumulation of intramyocellular lipids in diabetic rats. Our findings will be beneficial both for elucidating the mechanism of the antidiabetic activity of puerarin and for promoting the therapeutic potential of puerarin in the treatment of diabetes.
资助项目	Natural Science Foundation of Zhejiang Province of ChinaNatural Science Foundation of Zhejiang Province [LY13H290007]; Wenzhou Public Welfare Science and Technology Project [Y20170167]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81570775]
出版者	NATURE PUBLISHING GROUP
出版地	LONDON
ISSN	2044-4052
卷号	8 期号:1 页码:1
DOI	10.1038/s41387-017-0009-6
页数	13
WOS类目	Endocrinology & Metabolism ; Nutrition & Dietetics
WOS研究方向	Endocrinology & Metabolism ; Nutrition & Dietetics
WOS记录号	WOS:000474822100001
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
Pubmed记录号	29330446
PMC记录号	PMC5851431
Scopus记录号	2-s2.0-85047240167
通讯作者地址	[Gao, Qian]Center for Translational Medicine and Jiangsu Key Laboratory of Molecular Medicine,Medical School of Nanjing University,Nanjing,210093,China
scopus学科分类	Internal Medicine;Endocrinology, Diabetes and Metabolism
引用统计	被引频次：48[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/3866
专题	检验医学院（生命科学学院、生物学实验教学中心）_生物化学与分子生物学系基础医学院（机能实验教学中心）
通讯作者	Gao, Qian
作者单位	1.Center for Translational Medicine and Jiangsu Key Laboratory of Molecular Medicine,Medical School of Nanjing University,Nanjing,210093,China; 2.Department of Biochemistry,School of Basic Medical Sciences,Wenzhou Medical University,Wenzhou,325035,China; 3.Department of Anesthesiology,Affiliated Drum-Tower Hospital,Medical School of Nanjing University,Nanjing,210008,China
第一作者单位	基础医学院（机能实验教学中心）
推荐引用方式 GB/T 7714	Chen, Xiu-fang,Wang, Lei,Wu, Yong-zheng,et al. Effect of puerarin in promoting fatty acid oxidation by increasing mitochondrial oxidative capacity and biogenesis in skeletal muscle in diabetic rats[J]. NUTRITION & DIABETES,2018,8(1):1.
APA	Chen, Xiu-fang., Wang, Lei., Wu, Yong-zheng., Song, Shi-yu., Min, Hai-yan., ... & Gao, Qian. (2018). Effect of puerarin in promoting fatty acid oxidation by increasing mitochondrial oxidative capacity and biogenesis in skeletal muscle in diabetic rats. NUTRITION & DIABETES, 8(1), 1.
MLA	Chen, Xiu-fang,et al."Effect of puerarin in promoting fatty acid oxidation by increasing mitochondrial oxidative capacity and biogenesis in skeletal muscle in diabetic rats".NUTRITION & DIABETES 8.1(2018):1.