Nicotine-induced chondrogenic differentiation of human bone marrow stromal cells in vitro

doi:10.1007/s00167-012-1890-0

科研成果详情

题名	Nicotine-induced chondrogenic differentiation of human bone marrow stromal cells in vitro
作者	Ying, Xiaozhou ; Zhang, Wei ; Cheng, Shaowen ; Nie, Pengfei ; Cheng, Xiaojie ; Shen, Yue ; Wang, Wei ; Xue, Enxing ; Chen, Qingyu ; Kou, Dongquan ; Peng, Lei2,3; Zhang, Yu ; Lu, Chuanzhu 2
发表日期	2012-11
发表期刊	KNEE SURGERY SPORTS TRAUMATOLOGY ARTHROSCOPY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Bone marrow stromal stem cells (BMSCs) Chondrogenic Nicotine Type II collagen Aggrecan Cartilage tissue engineering
其他关键词	MESENCHYMAL STEM-CELLS ; ARTICULAR-CARTILAGE ; SMOKING ; ACETYLCHOLINE ; TRANSPLANTATION ; CHONDROCYTES ; METABOLISM ; EXPRESSION ; RECEPTORS ; TOXICITY
摘要	Nicotine has been reported that it has a dose-dependent effect on matrix mineralization by human bone marrow cells. However, there is no relevant research concerning on chondrogenic differentiation potential of bone marrow stromal stem cells (BMSCs) treated with nicotine in vitro. The aims of the study were to examine the effects of nicotine (0, 10(-7), 10(-6) and 10(-5) M) on the proliferation and chondrogenic differentiation of BMSCs from three healthy donors in vitro. BMSCs proliferation was analyzed by CCK8 assay and real-time polymerase chain reaction was used to assay the expression of type II collagen, aggrecan, type I collagen and type X collagen. The proteoglycan content was stained by Alcian blue, and the sulfated glycosaminoglycan (sGAG) content of BMSCs was quantified spectrofluorometrically using dimethylmethylene blue. The cell viability was not significantly impaired until up to a concentration of 10(-5) M nicotine. Nicotine promoted the proliferation and enhanced the expression of type II collagen at the level up to 10(-6) M (P < 0.05). The expression of aggrecan was reduced at the concentration of 10(-5) M nicotine at day 14 (P < 0.05), and there was no significant difference in aggrecan gene expression at 10(-7) and 10(-6) M nicotine levels compared to control group (n.s.). Also the fibroblastic and hypertrophic gene expressions were down-regulated in the chondrogenic medium with 10(-7)-10(-5) M nicotine (P < 0.05). It was implied that local application of nicotine at an appropriate concentration may be a promising approach for enhancing chondrogenic differentiation capacity of BMSCs in cell-based cartilage tissue engineering. Also these results indicate that nicotine maybe a potentially useful drug for the treatment of Osteoarthritis.
资助项目	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31010121]; Hainan Provincial Health Department [2011-34]; Medicines and Health Research fund of Zhejiang [2007B147]; Zhejiang Science and Technology Project [2010C34G2090013]; Wenzhou Technology Project [Y20090288, Y20070133]
出版者	SPRINGER
出版地	NEW YORK
ISSN	0942-2056
EISSN	1433-7347
卷号	20 期号:11 页码:2329-2336
DOI	10.1007/s00167-012-1890-0
页数	8
WOS类目	Orthopedics ; Sport Sciences ; Surgery
WOS研究方向	Orthopedics ; Sport Sciences ; Surgery
WOS记录号	WOS:000310223100031
收录类别	SCIE ; PUBMED
URL	查看原文
PubMed ID	22258655
通讯作者地址	[Zhang, Yu]Second Affiliated Hosp, Dept Orthopaed Surg, Wenzhou Med Coll, 109 Xue Yuan Xi Rd, Wenzhou, Peoples R China.
TOP期刊	TOP期刊
引用统计	被引频次：11[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/3789
专题	温州医科大学附属第二医院_创伤外科
通讯作者	Zhang, Yu
作者单位	1.Second Affiliated Hosp, Dept Orthopaed Surg, Wenzhou Med Coll, Wenzhou, Peoples R China; 2.Affiliated Hosp, Hainan Med Coll, Ctr Trauma, Haikou 571100, Peoples R China; 3.Affiliated Hosp, Hainan Med Coll, Affiliated Hosp 2, Wenzhou Med Coll,Trauma Ctr, Wenzhou 325000, Peoples R China
通讯作者单位	温州医科大学
推荐引用方式 GB/T 7714	Ying, Xiaozhou,Zhang, Wei,Cheng, Shaowen,et al. Nicotine-induced chondrogenic differentiation of human bone marrow stromal cells in vitro[J]. KNEE SURGERY SPORTS TRAUMATOLOGY ARTHROSCOPY,2012,20(11):2329-2336.
APA	Ying, Xiaozhou., Zhang, Wei., Cheng, Shaowen., Nie, Pengfei., Cheng, Xiaojie., ... & Lu, Chuanzhu. (2012). Nicotine-induced chondrogenic differentiation of human bone marrow stromal cells in vitro. KNEE SURGERY SPORTS TRAUMATOLOGY ARTHROSCOPY, 20(11), 2329-2336.
MLA	Ying, Xiaozhou,et al."Nicotine-induced chondrogenic differentiation of human bone marrow stromal cells in vitro".KNEE SURGERY SPORTS TRAUMATOLOGY ARTHROSCOPY 20.11(2012):2329-2336.