Baicalein enhances the osteogenic differentiation of human periodontal ligament cells by activating the Wnt/I beta-catenin signaling pathway

doi:10.1016/j.archoralbio.2017.01.019

科研成果详情

题名	Baicalein enhances the osteogenic differentiation of human periodontal ligament cells by activating the Wnt/I beta-catenin signaling pathway
作者	Chen, Li-Jiao1; Hu, Bi-Bo 1; Shi, Xin-Lian 2; Ren, Man-Man 2; Yu, Wen-Bin1; Cen, Sheng-Dan 2; Hu, Rong-Dang1; Deng, Hui2,3
发表日期	2017-06
发表期刊	ARCHIVES OF ORAL BIOLOGY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Baicalein Osteogenic differentiation Wnt/I beta-catenin signaling Periodontal ligament cells
其他关键词	OSTEOBLAST DIFFERENTIATION ; STEM-CELLS ; IN-VITRO ; SCUTELLARIA-BAICALENSIS ; TRANSCRIPTION FACTORS ; GENE-EXPRESSION ; BONE-FORMATION ; DISEASE ; INHIBITION ; RUNX2
摘要	Objective: Periodontium regeneration is one of the most important processes for periodontitis therapy. Human periodontal ligament cells (hPDLCs) play a vital role in the repair and regeneration of periodontal tissues. Our study aimed to investigated the mechanisms underlying the promotion of hPLDCs osteogenic differentiation by baicalein. Design: hPDLCs were obtained from periodontal ligament (PDL) tissues by primary culture. The MIT assay was used to determine the growth curves of hPDLCs treated with different concentrations of baicalein (1.25, 2.5, 5, or 10 M). Alkaline phosphatase (ALP) staining and Alizarin red S staining were performed to assess osteogenic differentiation of hPDLCs administered baicalein. Osteogenic differentiation-related gene and protein expression levels and Wnt/beta-catenin pathway signal changes were assessed by qRT-PCR and Western blotting analysis. Results: The results showed that baicalein decreased the growth of hPDLCs slightly and increased ALP activity and calcium deposition in a dose-dependent manner. The expression of runt-related transcription factor 2 (RUNX2), bone morphogenetic protein 2 (BMP2), Osterix (OSX) and osteocalcin (OCN) were elevated after baicalein administration. Moreover, baicalein strongly activated the Wnti beta-catenin pathway and up-regulated the expression of beta-catenin, lymphoid enhancer factor 1 (LEFI) and Cyclin Dl. Dickkopf-related protein 1 (DKK-1) significantly reversed the effects of baicalein on hPDLCs. Conclusions: Our findings indicated that baicalein enhanced the osteogenic differentiation of hPDLCs via the activation of the Wnt beta-catenin signaling pathway, which may represent a potential candidate for periodontitis therapy. (C) 2017 Elsevier Ltd. All rights reserved.
资助项目	Zhejiang Provincial Natural Science Foundation of ChinaNatural Science Foundation of Zhejiang Province [LY17H140009]; Zhejiang Provincial Traditional Chinese Medicine Science and Technology Plan [2015ZA122, 2017ZB068]; Public Welfare Science and Technology Plan of Wenzhou City [Y20150073, Y20160140]
出版者	PERGAMON-ELSEVIER SCIENCE LTD
出版地	OXFORD
ISSN	0003-9969
EISSN	1879-1506
卷号	78 页码:100-108
DOI	10.1016/j.archoralbio.2017.01.019
页数	9
WOS类目	Dentistry, Oral Surgery & Medicine
WOS研究方向	Dentistry, Oral Surgery & Medicine
WOS记录号	WOS:000400719000014
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	28222387
SCOPUSEID	2-s2.0-85013077378
通讯作者地址	[Hu, Rong-Dang]Department of Orthodontics,School of Stomatology,Wenzhou Medical University,Wenzhou,China
Scopus学科分类	Otorhinolaryngology;Dentistry (all);Cell Biology
引用统计	被引频次：36[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/3590
专题	口腔医学院、附属口腔医院_口腔正畸
通讯作者	Hu, Rong-Dang
作者单位	1.Department of Orthodontics,School of Stomatology,Wenzhou Medical University,Wenzhou,China; 2.Department of Periodontics,School of Stomatology,Wenzhou Medical University,Wenzhou,China; 3.Department of Orthodontics,School of Dentistry,University of Queensland,Brisbane,Australia
第一作者单位	口腔医学院、附属口腔医院
通讯作者单位	口腔医学院、附属口腔医院
第一作者的第一单位	口腔医学院、附属口腔医院
推荐引用方式 GB/T 7714	Chen, Li-Jiao,Hu, Bi-Bo,Shi, Xin-Lian,et al. Baicalein enhances the osteogenic differentiation of human periodontal ligament cells by activating the Wnt/I beta-catenin signaling pathway[J]. ARCHIVES OF ORAL BIOLOGY,2017,78:100-108.
APA	Chen, Li-Jiao., Hu, Bi-Bo., Shi, Xin-Lian., Ren, Man-Man., Yu, Wen-Bin., ... & Deng, Hui. (2017). Baicalein enhances the osteogenic differentiation of human periodontal ligament cells by activating the Wnt/I beta-catenin signaling pathway. ARCHIVES OF ORAL BIOLOGY, 78, 100-108.
MLA	Chen, Li-Jiao,et al."Baicalein enhances the osteogenic differentiation of human periodontal ligament cells by activating the Wnt/I beta-catenin signaling pathway".ARCHIVES OF ORAL BIOLOGY 78(2017):100-108.