Bioglass promotes wound healing by inhibiting endothelial cell pyroptosis through regulation of the connexin 43/reactive oxygen species (ROS) signaling pathway

doi:10.1038/s41374-021-00675-6

科研成果详情

题名	Bioglass promotes wound healing by inhibiting endothelial cell pyroptosis through regulation of the connexin 43/reactive oxygen species (ROS) signaling pathway
作者	Zhang, Kailun 1; Chai, Bo 2; Ji, Hao 1; Chen, liuqing 1; Ma, Yanbing 1; Zhu, Lifei2; Xu, Jingyu 1; Wu, Yanqing 1; Lan, Yinan 3; Li, Hao 4; Feng, Zhiguo 2; Xiao, Jian2; Zhang, Hongyu2; Xu, Ke 1
发表日期	2022-01
发表期刊	LABORATORY INVESTIGATION 影响因子和分区
语种	英语
原始文献类型	Article
其他关键词	STEM-CELLS ; TISSUE ; ANGIOGENESIS ; IMPROVES ; VASCULARIZATION ; INFLAMMASOMES ; COMPOSITES ; CONSTRUCTS ; INJURY ; BRAIN
摘要	Bioactive glass (BG) has recently shown great promise in soft tissue repair, especially in wound healing; however, the underlying mechanism remains unclear. Pyroptosis is a novel type of programmed cell death that is involved in various traumatic injury diseases. Here, we hypothesized that BG may promote wound healing through suppression of pyroptosis. To test this scenario, we investigated the possible effect of BG on pyroptosis in wound healing both in vivo and in vitro. This study showed that BG can accelerate wound closure, granulation formation, collagen deposition, and angiogenesis. Moreover, western blot analysis and immunofluorescence staining revealed that BG inhibited the expression of pyroptosis-related proteins in vivo and in vitro. In addition, while BG regulated the expression of connexin43 (Cx43), it inhibited reactive oxygen species (ROS) production. Cx43 activation and inhibition experiments further indicate that BG inhibited pyroptosis in endothelial cells by decreasing Cx43 expression and ROS levels. Taken together, these studies suggest that BG promotes wound healing by inhibiting pyroptosis via Cx43/ROS signaling pathway. In this study, the authors demonstrated that bioactive glass promotes granulation formation, collagen deposition and angiogenesis to accelerate wound healing. Bioactive glass down-regulaties the connexin 43/reactive oxygen species signaling pathway to inhibit endothelial cell pyroptosis in vitro and in vivo.
资助项目	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81802251, 81772450, 81801233]; Zhejiang Provincial Natural Science Foundation of ChinaNatural Science Foundation of Zhejiang Province [LQ18H150003, LQ18H090011, LY19H150001 LY17H090014, LWY20H310001]; Zhejiang Provincial Medical and Health Science and Technology Fund [2018KY186]; Wenzhou science and technology innovation project [ZY2020023, ZY2020026]
出版者	SPRINGERNATURE
出版地	LONDON
ISSN	0023-6837
EISSN	1530-0307
卷号	102 期号:1 页码:90-101
DOI	10.1038/s41374-021-00675-6
页数	12
WOS类目	Medicine, Research & Experimental ; Pathology
WOS研究方向	Research & Experimental Medicine ; Pathology
WOS记录号	WOS:000695765600002
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	34521991
SCOPUSEID	2-s2.0-85115169985
通讯作者地址	[Xu, Ke]Institute of Life Sciences,Engineering Laboratory of Zhejiang province for pharmaceutical development of growth factors,Biomedical Collaborative Innovation Center of Wenzhou,Wenzhou University,Zhejiang,China ; [Xiao, Jian]School of Pharmaceutical Sciences,Wenzhou Wound Repair and Regeneration Key Laboratory,Cixi Biomedical Research Institute,Wenzhou Medical University,Zhejiang,China ; [Zhang, Hongyu]School of Pharmaceutical Sciences,Wenzhou Wound Repair and Regeneration Key Laboratory,Cixi Biomedical Research Institute,Wenzhou Medical University,Zhejiang,China
Scopus学科分类	Medicine (all)
引用统计	被引频次：4[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/3519
专题	药学院（分析测试中心）研究生工作部（研究生院）第一临床医学院（信息与工程学院）、附属第一医院_外科学_整形外科其他_附属第六医院（丽水市人民医院）其他_附属第五医院（丽水市中心医院）
通讯作者	Xiao, Jian; Zhang, Hongyu; Xu, Ke
作者单位	1.Institute of Life Sciences,Engineering Laboratory of Zhejiang province for pharmaceutical development of growth factors,Biomedical Collaborative Innovation Center of Wenzhou,Wenzhou University,Zhejiang,China; 2.School of Pharmaceutical Sciences,Wenzhou Wound Repair and Regeneration Key Laboratory,Cixi Biomedical Research Institute,Wenzhou Medical University,Zhejiang,China; 3.Department of Orthopedic Surgery,Lishui Central Hospital,The Fifth Affiliated Hospital of Wenzhou Medical University,Zhejiang,China; 4.Department of Orthopedics Surgery,Lishui People’s Hospital,The sixth affiliated hospital of Wenzhou medical university,Lishui,Zhejiang,China
通讯作者单位	药学院（分析测试中心）
推荐引用方式 GB/T 7714	Zhang, Kailun,Chai, Bo,Ji, Hao,et al. Bioglass promotes wound healing by inhibiting endothelial cell pyroptosis through regulation of the connexin 43/reactive oxygen species (ROS) signaling pathway[J]. LABORATORY INVESTIGATION,2022,102(1):90-101.
APA	Zhang, Kailun., Chai, Bo., Ji, Hao., Chen, liuqing., Ma, Yanbing., ... & Xu, Ke. (2022). Bioglass promotes wound healing by inhibiting endothelial cell pyroptosis through regulation of the connexin 43/reactive oxygen species (ROS) signaling pathway. LABORATORY INVESTIGATION, 102(1), 90-101.
MLA	Zhang, Kailun,et al."Bioglass promotes wound healing by inhibiting endothelial cell pyroptosis through regulation of the connexin 43/reactive oxygen species (ROS) signaling pathway".LABORATORY INVESTIGATION 102.1(2022):90-101.