题名 | Metal-organic framework-encapsulated micellar silver nanoparticles for tumor microenvironment-adaptive electrochemical determination of matrix metalloproteinase-2 |
作者 | |
发表日期 | 2022-04-10 |
发表期刊 | Electrochimica Acta 影响因子和分区 |
语种 | 英语 |
原始文献类型 | Journal article (JA) |
关键词 | Chemical detection Coatings Diagnosis Diseases Metal-Organic Frameworks Organometallics Peptides Silver nanoparticles Tumors Cancer diagnosis Electrochemical determination Electrochemical techniques Extracellular microenvironment Matrix metalloproteinase-2 Metalorganic frameworks (MOFs) Micellar silver nanoparticle Micellars Silver cores Tumor microenvironments |
其他关键词 | TRANSCRIPTION ; BIOSENSORS ; CLEAVAGE ; ASSAY ; MMP-2 |
摘要 | Matrix metalloproteinase 2, an important modulator of tumor microenvironment, has received significant attention as a biomarker for cancer diagnosis. In this paper, we report a tumor microenvironment-adaptive electrochemical method for sensitive determination of matrix metalloproteinase 2. Metal-organic framework-encapsulated micellar silver nanoparticles, mAgNPs@MOF, are the central element of the method, and are prepared through the coating of micellar silver nanoparticles with zeolitic imidazolate framework-8. Benefiting from inherent pH-responsive ability of MOF coating, mAgNPs@MOF disassemble in the tumor microenvironment at an acidic pH, releasing micellar silver nanoparticles that are consisted of a peptide shell and a silver core. Then, matrix metalloproteinase 2 presented in the tumor microenvironment selectively recognizes and cleaves the peptide shell of micellar silver nanoparticles, which promotes the accumulation of silver cores onto magnetic beads, thereby leading to amplified electrochemical responses. By contrast, in the normal extracellular microenvironment, stable MOF coating prevents matrix metalloproteinase 2 from approaching mAgNPs, and thus inhibits the generation of unexpected interfering signals. Experiment results demonstrate that the method not only displays a wide linear range from 50 fg/mL to 20 ng/mL and a detection limit as low as 20.8 fg/mL for enzymatic assay, but also exhibits high selectivity and anti-interference performance in the extracellular microenvironment of tumor cells. Therefore, the method may pave a valuable way for monitoring enzymatic activity in the tumor microenvironment, and imply a potential use in cancer diagnosis in future. © 2022 |
资助项目 | Wenzhou Science and Technology Bureau Project [Y20190143]; Zhejiang Natural Science Foundation Project [LY18H160054]; National Natural Science Foundation of China [81972799] |
出版者 | Elsevier Ltd |
出版地 | OXFORD |
ISSN | 0013-4686 |
EISSN | 1873-3859 |
卷号 | 411 |
DOI | 10.1016/j.electacta.2022.140100 |
页数 | 9 |
WOS类目 | Electrochemistry |
WOS研究方向 | Electrochemistry |
WOS记录号 | WOS:000780754900004 |
收录类别 | EI ; SCIE ; SCOPUS |
EI入藏号 | 20220911739063 |
EI主题词 | Metal nanoparticles |
EI分类号 | 461.2 Biological Materials and Tissue Engineering ; 461.6 Medicine and Pharmacology ; 461.9 Biology ; 531.1 Metallurgy ; 761 Nanotechnology ; 801 Chemistry ; 804.1 Organic Compounds ; 813.2 Coating Materials |
URL | 查看原文 |
Scopus记录号 | 2-s2.0-85125452382 |
通讯作者地址 | [Zhao, Jing]Center for Molecular Recognition and Biosensing,Shanghai Engineering Research Center of Organ Repair,School of Life Sciences,Shanghai University,Shanghai,200444,China |
scopus学科分类 | Chemical Engineering (all);Electrochemistry |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | https://kms.wmu.edu.cn/handle/3ETUA0LF/35043 |
专题 | 附属第一医院_神经外科 |
通讯作者 | Zhao, Jing |
作者单位 | 1.Department of Neurosurgery,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325000,China; 2.Center for Molecular Recognition and Biosensing,Shanghai Engineering Research Center of Organ Repair,School of Life Sciences,Shanghai University,Shanghai,200444,China; 3.Department of Breast Surgery,Fudan University Shanghai Cancer Center,Key Laboratory of Breast Cancer in Shanghai,Shanghai,200032,China |
第一作者单位 | 附属第一医院; 第一临床医学院(信息与工程学院)、附属第一医院 |
第一作者的第一单位 | 附属第一医院 |
推荐引用方式 GB/T 7714 | Wang, Ying,Sha, Lingjun,Mao, Huiru,et al. Metal-organic framework-encapsulated micellar silver nanoparticles for tumor microenvironment-adaptive electrochemical determination of matrix metalloproteinase-2[J]. Electrochimica Acta,2022,411. |
APA | Wang, Ying, Sha, Lingjun, Mao, Huiru, Zhao, Jing, & Tu, Ming. (2022). Metal-organic framework-encapsulated micellar silver nanoparticles for tumor microenvironment-adaptive electrochemical determination of matrix metalloproteinase-2. Electrochimica Acta, 411. |
MLA | Wang, Ying,et al."Metal-organic framework-encapsulated micellar silver nanoparticles for tumor microenvironment-adaptive electrochemical determination of matrix metalloproteinase-2".Electrochimica Acta 411(2022). |
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