[A molecular epidemiological study of respiratory syncytial virus circulating in southern Zhejiang Province, China, from 2009 to 2014]

doi:10.7499/j.issn.1008-8830.2018.11.005

科研成果详情

题名	[A molecular epidemiological study of respiratory syncytial virus circulating in southern Zhejiang Province, China, from 2009 to 2014]
其他题名	A molecular epidemiological study of respiratory syncytial virus circulating in southern Zhejiang Province, China, from 2009 to 2014
作者	Yi-Wei Dong; Li-Hong Dai; Wen-Jing Ye; Xiao-Fang Chen; Lin Dong
发表日期	2018-11-01
发表期刊	Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Child G protein Genotype Respiratory syncytial virus Subtype
摘要	Objective:To find out the prevalence of respiratory syncytial virus (RSV) genotypes in southern Zhejiang Province, China, and to study the genetic characteristics of G protein from subtype A of RSV. Methods:The lower respiratory tract secretions of children under 5 years of age who were hospitalized for pneumonia and bronchiolitis in three hospitals in southern Zhejiang Province from July 2009 to June 2014 were collected. Direct immunofluorescence assay was used to detect RSV antigens from the collected secretions. A total of 200 samples were randomly selected from RSV-positive specimens in each prevailing year (from July of a specific year to June of the next year). RT-PCR was used to determine RSV subtypes, and the near-full length gene sequence of G protein from subtype A was amplified and sequenced to identify the genotype. Results:A total of 25 449 samples of lower respiratory tract secretions were collected from 2009 to 2014, among which 6 416 (25.21%) samples were RSV-positive. Among the 1 000 RSV-positive specimens randomly sampled, 462 strains (46.2%) were subtype A, and 538 strains (53.8%) were subtype B. Subtype A accounted for 22.5%, 74.5%, 84.5%, 19.0%, and 30.5% of the total strains in each year from 2009 to 2014. A total of 25 RSV subtype A strains were randomly sampled and sent out for bidirectional sequencing in each year, which confirmed 52 positive subtype A strains. Four genotypes of subtype A strains were obtained from the above strains, including NA1 (39 strains), NA4 (1 strain), ON1 (10 strains), and GA2 (2 strains). NA1 was the dominant genotype between 2009 and 2012, and ON1 was the only genotype of subtype A during 2013-2014. The nucleotide homology and amino acid homology between the G protein of subtype A and the prototype strain A2 were 80.7%-89.3% and 74.4%-82.6%, respectively. The nucleotide homology and amino acid homology between the isolates of subtype A were 81.5%-100% and 80.2%-100%, respectively. Conclusions:In southern Zhejiang Province from 2009 to 2014, there was a co-circulation of RSV subtypes A and B, as well as a co-circulation of several different genotypes of RSV subtype A, which had highly variable G protein genes.
其他摘要	Objective To find out the prevalence of respiratory syncytial virus (RSV) genotypes in southern Zhejiang Province, China, and to study the genetic characteristics of G protein from subtype A of RSV. Methods The lower respiratory tract secretions of children under 5 years of age who were hospitalized for pneumonia and bronchiolitis in three hospitals in southern Zhejiang Province from July 2009 to June 2014 were collected. Direct immunofluorescence assay was used to detect RSV antigens from the collected secretions. A total of 200 samples were randomly selected from RSV-positive specimens in each prevailing year (from July of a specific year to June of the next year). RT-PCR was used to determine RSV subtypes, and the near-full length gene sequence of G protein from subtype A was amplified and sequenced to identify the genotype. Results A total of 25 449 samples of lower respiratory tract secretions were collected from 2009 to 2014, among which 6 416 (25.21%) samples were RSV-positive. Among the 1 000 RSV-positive specimens randomly sampled, 462 strains (46.2%) were subtype A, and 538 strains (53.8%) were subtype B. Subtype A accounted for 22.5%, 74.5%, 84.5%, 19.0%, and 30.5% of the total strains in each year from 2009 to 2014. A total of 25 RSV subtype A strains were randomly sampled and sent out for bidirectional sequencing in each year, which confirmed 52 positive subtype A strains. Four genotypes of subtype A strains were obtained from the above strains, including NA1 (39 strains), NA4 (1 strain), ON1 (10 strains), and GA2 (2 strains). NA1 was the dominant genotype between 2009 and 2012, and ON1 was the only genotype of subtype A during 2013-2014. The nucleotide homology and amino acid homology between the G protein of subtype A and the prototype strain A2 were 80.7%-89.3% and 74.4%-82.6%, respectively. The nucleotide homology and amino acid homology between the isolates of subtype A were 81.5%-100% and 80.2%- 100%, respectively. Conclusions In southern Zhejiang Province from 2009 to 2014, there was a co-circulation of RSV subtypes A and B, as well as a co-circulation of several different genotypes of RSV subtype A, which had highly variable G protein genes.
资助项目	浙江省公益性技术应用研究项目 ; 国家临床重点专科开放项目
出版者	Xiangya Hospital of CSU141 Xiangya RoadChangsha Hunan410008
ISSN	1008-8830
卷号	20 期号:11 页码:904-910.
DOI	10.7499/j.issn.1008-8830.2018.11.005
页数	7
收录类别	PUBMED ; SCOPUS ; CSCD ; 万方 ; PKU ; 北大核心 ; ISTIC
学科领域	医药、卫生 ; 预防医学、卫生学 ; 儿科学
URL	查看原文
CSCD记录号	CSCD:6368613
PubMed ID	30477620
PMC记录号	PMC7389031
SCOPUSEID	2-s2.0-85057269022
Scopus学科分类	Pediatrics, Perinatology and Child Health
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/34828
专题	第二临床医学院，附属第二医院、育英儿童医院_内科学_呼吸内科
作者单位	Department of Pulmonology, Second Affiliated Hospital and Yuying Children&s Hospital, Wenzhou Medical University, Wenzhou, Zhejiang 325027, China
第一作者单位	第二临床医学院，附属第二医院、育英儿童医院_内科学_呼吸内科
第一作者的第一单位	第二临床医学院，附属第二医院、育英儿童医院_内科学_呼吸内科
推荐引用方式 GB/T 7714	Yi-Wei Dong,Li-Hong Dai,Wen-Jing Ye,et al. [A molecular epidemiological study of respiratory syncytial virus circulating in southern Zhejiang Province, China, from 2009 to 2014][J]. Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics,2018,20(11):904-910..
APA	Yi-Wei Dong, Li-Hong Dai, Wen-Jing Ye, Xiao-Fang Chen, & Lin Dong. (2018). [A molecular epidemiological study of respiratory syncytial virus circulating in southern Zhejiang Province, China, from 2009 to 2014]. Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics, 20(11), 904-910..
MLA	Yi-Wei Dong,et al."[A molecular epidemiological study of respiratory syncytial virus circulating in southern Zhejiang Province, China, from 2009 to 2014]".Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics 20.11(2018):904-910..

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