[Streptococcus pneumoniae induces SPLUNC1 and the regulatory effects of resveratrol]

doi:10.7499/j.issn.1008-8830.2017.01.018

科研成果详情

题名	[Streptococcus pneumoniae induces SPLUNC1 and the regulatory effects of resveratrol]
其他题名	Streptococcus pneumoniae induces SPLUNC1 and the regulatory effects of resveratrol
作者	Yan-Ping Shang; Li Lin; Chang-Chong Li
发表日期	2017
发表期刊	Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics 影响因子和分区
语种	英语
原始文献类型	期刊论文
关键词	短腭、肺及鼻咽上皮克隆1 白藜芦醇肺炎链球菌人支气管上皮细胞
其他关键词	Short palate ; lung ; and nasal epithelium clone 1 ; Resveratrol ; Streptococcus pneumoniae ; Human bronchial epithelial cell
摘要	Objective:To investigate the host-defense role of short palate, lung, and nasal epithelium clone 1 (SPLUNC1) in Streptococcus pneumoniae (SP) infection and the effect of resveratrol (Res) on SPLUNC1 expression, and to provide new thoughts for the treatment of diseases caused by SP infection. Methods:According to the multiplicity of infection (MOI), BEAS-2B cells with SP infection were divided into control group, MOI20 SP group, and MOI50 SP group. According to the different concentrations of Res, the BEAS-2B cells with MOI20 SP infection pretreated by Res were divided into 12.5Res+SP group, 25Res+SP group, and 50Res+SP group (the final concentrations of Res were 12.5, 25, and 50 μmol/L, respectively). Cell Counting Kit-8 was used to measure cell activity and determine the optimal concentration and action time of SP and Res. In the formal experiment, the cells were divided into control group, Res group, SP group, and Res+SP group. Real-time PCR and ELISA were used to measure the mRNA and protein expression of SPLUNC1. Results:Over the time of SP infection, cell activity tended to decrease. Compared with the control group and the MOI20 SP group, the MOI50 SP group had a reduction in cell activity. Compared with the MOI20 SP group, the 25Res+SP group had increased cell activity and the 50Res+SP group had reduced cell activity (P<0.05). MOI20 SP bacterial suspension and 25 μmol/L Res were used for the formal experiment. Over the time of SP infection, the mRNA expression of SPLUNC1 in BEAS-2B cells firstly increased and then decreased in the SP group and the Res+SP group (P<0.05). Compared with the SP group, the Res+SP group had significant increases in the mRNA and protein expression of SPLUNC1 at all time points (P<0.05). Compared with the control group, the Res group had no significant changes in the mRNA and protein expression of SPLUNC1 (P>0.05). Conclusions:SP infection can induce SPLUNC1 expression and the host-defense role of SPLUNC1. Res can upregulate SPLUNC1 expression during the development of infection and enhance cell protection in a concentration- and time-dependent manner.
其他摘要	Objective To investigate the host-defense role of short palate, lung, and nasal epithelium clone 1 (SPLUNC1) in Streptococcus pneumoniae (SP) infection and the effect of resveratrol (Res) on SPLUNC1 expression, and to provide new thoughts for the treatment of diseases caused by SP infection. Methods According to the multiplicity of infection (MOI), BEAS-2B cells with SP infection were divided into control group, MOI20 SP group, and MOI50 SP group. According to the different concentrations of Res, the BEAS-2B cells with MOI20 SP infection pretreated by Res were divided into 12.5Res+SP group, 25Res+SP group, and 50Res+SP group (the final concentrations of Res were 12.5, 25, and 50 μmol/L, respectively). Cell Counting Kit-8 was used to measure cell activity and determine the optimal concentration and action time of SP and Res. In the formal experiment, the cells were divided into control group, Res group, SP group, and Res+SP group. Real-time PCR and ELISA were used to measure the mRNA and protein expression of SPLUNC1. Results Over the time of SP infection, cell activity tended to decrease. Compared with the control group and the MOI20 SP group, the MOI50 SP group had a reduction in cell activity. Compared with the MOI20 SP group, the 25Res+SP group had increased cell activity and the 50Res+SP group had reduced cell activity (P<0.05). MOI20 SP bacterial suspension and 25 μmol/L Res were used for the formal experiment. Over the time of SP infection, the mRNA expression of SPLUNC1 in BEAS-2B cells firstly increased and then decreased in the SP group and the Res+SP group (P<0.05). Compared with the SP group, the Res+SP group had significant increases in the mRNA and protein expression of SPLUNC1 at all time points (P<0.05). Compared with the control group, the Res group had no significant changes in the mRNA and protein expression of SPLUNC1 (P >0.05). Conclusions SP infection can induce SPLUNC1 expression and the host-defense role of SPLUNC1. Res can upregulate SPLUNC1 expression during the development of infection and enhance cell protection in a concentration- and time-dependent manner.
资助项目	浙江省自然科学基金 ; 浙江省教育厅项目 ; 卫生部国家临床重点专科开放课题 ; 温州市2014公益性科技计划项目
出版者	Xiangya Hospital of CSU141 Xiangya RoadChangsha Hunan410008
ISSN	1008-8830
卷号	19 期号:1 页码:111-116.
DOI	10.7499/j.issn.1008-8830.2017.01.018
页数	6
收录类别	PUBMED ; CSCD ; 万方 ; SCOPUS ; PKU ; 北大核心 ; ISTIC
学科领域	医药、卫生 ; 基础医学
URL	查看原文
CSCD记录号	CSCD:5905306
PubMed ID	28100333
PMC记录号	PMC7390127
SCOPUSEID	2-s2.0-85018394641
Scopus学科分类	Pediatrics, Perinatology and Child Health
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/34825
专题	第二临床医学院，附属第二医院、育英儿童医院_儿科学_儿内呼吸
作者单位	Department of Pediatric Pulmonology, Second Affiliated Hospital/Yuying Children&s Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325027, China
第一作者单位	第二临床医学院，附属第二医院、育英儿童医院_儿科学_儿内呼吸
第一作者的第一单位	第二临床医学院，附属第二医院、育英儿童医院_儿科学_儿内呼吸
推荐引用方式 GB/T 7714	Yan-Ping Shang,Li Lin,Chang-Chong Li. [Streptococcus pneumoniae induces SPLUNC1 and the regulatory effects of resveratrol][J]. Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics,2017,19(1):111-116..
APA	Yan-Ping Shang, Li Lin, & Chang-Chong Li. (2017). [Streptococcus pneumoniae induces SPLUNC1 and the regulatory effects of resveratrol]. Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics, 19(1), 111-116..
MLA	Yan-Ping Shang,et al."[Streptococcus pneumoniae induces SPLUNC1 and the regulatory effects of resveratrol]".Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics 19.1(2017):111-116..

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