[Effects of pirfenidone on hepatic fibrosis in mice induced by carbon tetrachloride]

doi:10.13459/j.cnki.cjap.2016.04.023

科研成果详情

题名	[Effects of pirfenidone on hepatic fibrosis in mice induced by carbon tetrachloride]
其他题名	Effects of pirfenidone on hepatic fibrosis in mice induced by carbon tetrachloride
作者	Min Xiao; Xiao-Hu Qu; Jv-Ping Lv; Yang Shi; Chang-Xi Li; Ke-Jian Xie
发表日期	2016-04-08
发表期刊	Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology 影响因子和分区
语种	英语
原始文献类型	学术期刊
关键词	carbon tetrachloride hepatic fibrosis hepatic stellate cells mice pirfenidone.
其他关键词	pirfenidone ; carbon tetrachloride ; hepatic fibrosis ; hepatic stellate cells ; mice
摘要	Objective:To investigate the effects of pirfenidone on CCl4-induced liver fibrosis in mice. Methods:After 8-week feeding, 40 healthy male SPF ICR mice were randomly divided into 4 groups:liver fibrosis group (CCL4 group), low doses of Pirfenidone group (PFD-L group), high doses of Pirfenidone group (PFD-H group) and control group. The mice in CCL4 group, low doses of Pirfenidone group (PFD-L group), high doses of Pirfenidone group (PFD-H group) were injected intraperitoneally with 0.4 ml 10% CCL4 solution dissolved in soybean oil. Then the PFD-L and PFD-H groups were treated with 120 mg and 240 mg PFD via gastric gavage, respectively. Control group was injected with same volume of saline. Alanine aminotransferase(ALT), aspartate aminotransferase(AST), alkaline phosphatase(ALP) in serum were tested with automatic biochemistry analyzer and the pathologic changes of liver tissue were examined by HE staining. Furthermore, we identi-fied hyaluronic acids(HA), laminin(LN), collagentype IV(IV-C) in serum using radioimmunoassay and the expression of smooth muscle acti-nalpha(α-SMA) related gene in liver was tested by real-time fluorescence quantitative PCR. Results:Compared with control group, hepatic lobules in CCL4 mice were damaged significantly, collagenous fiber was deposited obviously, and counterfeit hepatic lobules formed. The serum levels of ALT, AST, ALP were increased obviously (P<0.05) with the enhancement of HA, LN, IV-C in serum (P<0.05) and the ex-pression of α-SMA related gene (P<0.05). Compared to CCL4-treated mice, the serum levels of ALT, AST, ALP in PFD-L and PFD-H groups were decreased, HA, LN, IV-C in PFD-L and PFD-H mice went down obviously,and the expression of α-SMA related gene was con-trolled (P<0.05). From pathological observation, we found the degree of liver fibrosis in PFD-L mice was reduced and collagenous fiber was decreased, only a little counterfeit hepatic lobule could be found. Cell arrangement in PFD-H mice recovered, the structural of hepatic lobules disordered and no obvious counterfeit hepatic lobules were found. Therefore, the recovery of PFD-H group was better than PFD-L group. Conclusions:Pirfenidone has a protective role in improving the outcome of the liver fibrosis and it may become a new direction of early intervention in liver fibrosis.
其他摘要	Objective: To investigate the effects of pirfenidone on CCl_4-induced liver fibrosis in mice. Methods: After 8-week feeding, 40 healthy male SPF ICR mice were randomly divided into 4 groups: liver fibrosis group (CCL_4 group),low doses of Pirfenidone group (PFD-L group), high doses of Pirfenidone group (PFD-H group) and control group. The mice in CCL_4 group, low doses of Pirfenidone group (PFD-L group), high doses of Pirfenidone group (PFD-H group) were injected intraperitoneally with 0.4 ml 10% CCL_4 solution dissolved in soybean oil. Then the PFD-L and PFD-H groups were treated with 120 mg and 240 mg PFD via gastric gavage, respectively. Control group was injected with same volume of saline. Alanine aminotransferase(ALT), aspartate aminotransferase(AST), alkaline phosphatase(ALP) in serum were tested with automatic biochemistry analyzer and the pathologic changes of liver tissue were examined by HE staining. Furthermore, we identified hyaluronic acids(HA), laminin(LN), collagentype Ⅳ(IV-C) in serum using radioimmunoassay and the expression of smooth muscle acti-nalpha(a-SMA) related gene in liver was tested by real-time fluorescence quantitative PCR. Results: Compared with control group, hepatic lobules in CCL_4 mice were damaged significantly, collagenous fiber was deposited obviously, and counterfeit hepatic lobules formed. The serum levels of ALT, AST, ALP were increased obviously (P < 0.05) with the enhancement of HA, LN, Ⅳ-C in serum (P < 0.05) and the expression of a-SMA related gene (P < 0.05). Compared to CCL_4-treated mice, the serum levels of ALT, AST, ALP in PFD-L and PFD-H groups were decreased, HA, LN, IV-C in PFD-L and PFD-H mice went down obviously, and the expression of a-SMA related gene was controlled (P < 0.05). From pathological observation, we found the degree of liver fibrosis in PFD-L mice was reduced and collagenous fiber was decreased, only a little counterfeit hepatic lobule could be found. Cell arrangement in PFD-H mice recovered, the structural of hepatic lobules disordered and no obvious counterfeit hepatic lobules were found. Therefore, the recovery of PFD-H group was better than PFD-L group. Conclusion:Pirfenidone has a protective role in improving the outcome of the liver fibrosis and it may become a new direction of early intervention in liver fibrosis.
资助项目	2015年度浙江省公益技术应用研究计划（实验动物）项目（2015C37099）
ISSN	1000-6834
卷号	32 期号:4 页码:378-382.
DOI	10.13459/j.cnki.cjap.2016.04.023
页数	5
收录类别	PUBMED ; CNKI ; SCOPUS ; 万方 ; CSCD ; PKU ; 北大核心 ; ISTIC
学科领域	医药、卫生 ; 内科学
URL	查看原文
CSCD记录号	CSCD:5763276
PubMed ID	29931966
SCOPUSEID	2-s2.0-85055078467
Scopus学科分类	Medicine (all)
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/34093
专题	基础医学院（机能实验教学中心）_生物科学系
作者单位	Department of Biology, School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou 325035, China.
第一作者单位	检验医学院（生命科学学院、生物学实验教学中心）
第一作者的第一单位	检验医学院（生命科学学院、生物学实验教学中心）
推荐引用方式 GB/T 7714	Min Xiao,Xiao-Hu Qu,Jv-Ping Lv,et al. [Effects of pirfenidone on hepatic fibrosis in mice induced by carbon tetrachloride][J]. Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology,2016,32(4):378-382..
APA	Min Xiao, Xiao-Hu Qu, Jv-Ping Lv, Yang Shi, Chang-Xi Li, & Ke-Jian Xie. (2016). [Effects of pirfenidone on hepatic fibrosis in mice induced by carbon tetrachloride]. Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology, 32(4), 378-382..
MLA	Min Xiao,et al."[Effects of pirfenidone on hepatic fibrosis in mice induced by carbon tetrachloride]".Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology 32.4(2016):378-382..

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