[AdipoRon for the treatment of type 2 diabetes in mice and its possible mechanism of the liver]

doi:10.13459/j.cnki.cjap.2016.03.002

科研成果详情

题名	[AdipoRon for the treatment of type 2 diabetes in mice and its possible mechanism of the liver]
其他题名	AdipoRon for the treatment of type 2 diabetes in mice and its possible mechanism of the liver
作者	Min Xiao; Xiao-Hu Qu; Chang-Xi Li; Ju-Ping Lv; Yang Shi; Ke-Jian Xie
发表日期	2016-03-08
发表期刊	Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology 影响因子和分区
语种	英语
原始文献类型	学术期刊
关键词	adiponectin receptor excitomotor liver cell injury mice type 2 diabetes.
其他关键词	type 2 diabetes ; adiponectin receptor excitomotor ; liver cell injury ; mice
摘要	Objective:To observe the effect of AdipoRon for the treatment of type 2 diabetes (T2DM)in mice and its effect on the liver. Methods:Forty male C57/BL6 mice (SPF) were randomly divided to normal control (NC) group and the experimental group. To establish the T2DM mice model, mice in the experimental group were fed with high fat and high glucose, combined with intraperitoneal injection of streptozotocin (STZ) in small doses, and mice were further subdivided into model control (DM) group, model control with low AdipoRon (DM+L) group and model control with high AdipoRon (DM+H) group (n=10). Serum indexes, such as levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) were detected biochemically and the morphological changes of liver cells were observed with HE staining and expression of liver carbohydrate related gene (PEPCK) were determined by real-time fluorescence quantitative PCR (real time FQ-PCR). Results:Compared with mice in the DM group, levels of ALT, AST, ALP, triglyceride (TG), glucose (GLU) reduced in DM+L and DM+H group (P<0.05). Concentrations of serum free fatty acids (FFA) in DM+L and DM+H group reduced significantly (P<0.05). Besides, concentrations of liver glucose-6-phosphatase (G-6-P) in the mice of DM+L group reduced significantly, while there was no significant difference in the content of G-6-P between the mice of DM+H group and the mice of DM group. Furthermore, the expression of the liver phosphoenolpyruvate carboxylase (PEPCK) in the DM+H group reduced significantly (P<0.05). Compared with the DM group no significant change was found in the PEPCK expression between DM+L and DM group. Conclusions:The serum indexes such as levels of ALT, AST, ALP, TG, Glu, G-6-P and PEPCK were all reduced in DM mice treated with AdipoRon, indicating the obvious protecting effect of AdipoRon on the liver in DM mice.
其他摘要	Objective: To observe the effect of AdipoRon for the treatment of type 2 diabetes (T2DM)in mice and its effect on the liver. Methods: Forty male C57/BL6 mice (SPF)were randomly divided to normal control (NC)group and the experimental group. To establish the T2DM mice model,mice in the experimental group were fed with high fat and high glucose,combined with intraperitoneal injection of streptozotocin (STZ)in small doses,and mice were further subdivided into model control (DM)group,model control with low AdipoRon (DM +L)group and model control with high AdipoRon (DM+H)group (n =10). Serum indexes,such as levels of alanine aminotransferase (ALT),aspartate aminotransferase (AST),alkaline phosphatase (ALP)were detected biochemically and the morphological changes of liver cells were observed with HE staining and expression of liver carbohydrate related gene (PEPCK)were determined by real-time fluorescence quantitative PCR (real time FQ-PCR). Results: Compared with mice in the DM group,levels of ALT,AST,ALP,triglyceride (TG),glucose (GLU)reduced in DM+L and DM+H group (P <0.05). Concentrations of serum free fatty acids (FFA)in DM+L and DM+H group reduced significantly (P <0.05). Besides,concentrations of liver glucose-6-phosphatase (G- 6-P)in the mice of DM+L group reduced significantly,while there was no significant difference in the content of G-6-P between the mice of DM+H group and the mice of DM group. Furthermore,the expression of the liver phosphoenolpyruvate carboxylase (PEPCK)in the DM +H group reduced significantly (P <0.05). Compared with the DM group no significant change was found in the PEPCK expression between DM+L and DM group. Conclusion: The serum indexes such as levels of ALT,AST,ALP,TG,Glu,G-6-P and PEPCK were all reduced in DM mice treated with AdipoRon,indicating the obvious protecting effect of AdipoRon on the liver in DM mice.
资助项目	2015年度浙江省公益技术应用研究计划（实验动物）项目（2015C37099）
ISSN	1000-6834
卷号	32 期号:3 页码:198-201.
DOI	10.13459/j.cnki.cjap.2016.03.002
页数	6
收录类别	PUBMED ; CNKI ; SCOPUS ; 万方 ; CSCD ; PKU ; 北大核心 ; ISTIC
学科领域	医药、卫生 ; 内科学
URL	查看原文
CSCD记录号	CSCD:5711263
PubMed ID	29931876
SCOPUSEID	2-s2.0-85055081205
Scopus学科分类	Medicine (all)
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/34088
专题	基础医学院（机能实验教学中心）_生物科学系
作者单位	Department of Biology, School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou 325035, China.
第一作者单位	检验医学院（生命科学学院、生物学实验教学中心）
第一作者的第一单位	检验医学院（生命科学学院、生物学实验教学中心）
推荐引用方式 GB/T 7714	Min Xiao,Xiao-Hu Qu,Chang-Xi Li,et al. [AdipoRon for the treatment of type 2 diabetes in mice and its possible mechanism of the liver][J]. Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology,2016,32(3):198-201..
APA	Min Xiao, Xiao-Hu Qu, Chang-Xi Li, Ju-Ping Lv, Yang Shi, & Ke-Jian Xie. (2016). [AdipoRon for the treatment of type 2 diabetes in mice and its possible mechanism of the liver]. Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology, 32(3), 198-201..
MLA	Min Xiao,et al."[AdipoRon for the treatment of type 2 diabetes in mice and its possible mechanism of the liver]".Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology 32.3(2016):198-201..

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