Relationship between the Number of Neutrophils and Myofibroblasts during Diabetic Wound Healing and Wound Age

doi:10.12116/j.issn.1004-5619.2019.02.004

科研成果详情

题名	Relationship between the Number of Neutrophils and Myofibroblasts during Diabetic Wound Healing and Wound Age
其他题名	Relationship between the Number of Neutrophils and Myofibroblasts during Diabetic Wound Healing and Wound Age
作者	J J Huang 1,2,3; Y Yao 1,2,3; C J Xia 1,2,3; Y D Zhao 1,2,3; S Yu 1,2,3; Y Gao1,2,3; G H Ye 1,2,3; L S Yu 1,2,3; Y Y Fan 1,2,3
发表日期	2019-04-01
发表期刊	Fa yi xue za zhi 影响因子和分区
语种	英语
原始文献类型	Article
关键词	forensic pathology diabetes mellitus wound healing neutrophil myofibroblast wound age estimation mice.
摘要	Objective To investigate the sequential changes of the number of neutrophils and myofibroblasts during diabetic wound healing, and discuss its application value in wound age estimation. Methods Diabetic DB mice and mice of the same age in the normal control group were selected, a wound healing model was established, wound samples were taken at different time points, while the number of neutrophils and myofibroblasts during diabetic wound healing were determined by immunohistochemical staining technique. Results The number of infiltrated neutrophils in the wounds of control and diabetic groups reached the peak respectively at 12 h and 5 d after injury. Compared with the control group, the number of neutrophils in the diabetic group decreased significantly from 6 h to 1 d after injury, but increased markedly from 5 d to 14 d. From 5 d to 10 d after injury, the average number of neutrophils at high magnification in wounds of the diabetic group was over 30, while that of neutrophils in wounds of the control group was less than 20. Myofibroblasts appeared in wounds from 3 d to 14 d after injury in the control group and from 5 d to 14 d after injury in the diabetic group. The difference in the number of myofibroblasts in wounds between control group and diabetic group from 3 to 7 d after injury had statistical significance. Conclusion In comparison with normal wound healing, the number of neutrophils and myofibroblasts during diabetic wound healing shows different sequential changes. The results of this study can provide reference for wound age estimation of patients with severe diabetes.
其他摘要	Objective To investigate the sequential changes of the number of neutrophils and myofibroblasts during diabetic wound healing, and discuss its application value in wound age estimation. Methods Diabetic DB mice and mice of the same age in the normal control group were selected, a wound healing model was established, wound samples were taken at different time points, while the number of neutrophils and myofibroblasts during diabetic wound healing were determined by immunohistochemical staining technique. Results The number of infiltrated neutrophils in the wounds of control and diabetic groups reached the peak respectively at 12 h and 5 d after injury. Compared with the control group, the number of neutrophils in the diabetic group decreased significantly from 6 h to 1 d after injury, but increased markedly from 5 d to 14 d. From 5 d to 10 d after injury, the average number of neutrophils at high magnification in wounds of the diabetic group was over 30, while that of neutrophils in wounds of the control group was less than 20. Myofibroblasts appeared in wounds from 3 d to 14 d after injury in the control group and from 5 d to 14 d after injury in the diabetic group. The difference in the number of myofibroblasts in wounds between control group and diabetic group from 3 to 7 d after injury had statistical significance. Conclusion In comparison with normal wound healing, the number of neutrophils and myofibroblasts during diabetic wound healing shows different sequential changes. The results of this study can provide reference for wound age estimation of patients with severe diabetes.
资助项目	国家自然科学基金 ; 浙江省自然科学基金 ; 上海市法医学重点实验室(司法鉴定科学研究院)资助项目 ; 温州医科大学基础研究发展课题资助项目
ISSN	1004-5619
卷号	35 期号:2 页码:149-153.
DOI	10.12116/j.issn.1004-5619.2019.02.004
页数	5
收录类别	PUBMED ; SCOPUS ; CSCD ; 万方 ; ISTIC
URL	查看原文
CSCD记录号	CSCD:6496834
PubMed ID	31135107
SCOPUSEID	2-s2.0-85066447545
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/34001
专题	基础医学院（机能实验教学中心）_法医学系温州医科大学
作者单位	1.Department of Forensic Medicine, Wenzhou Medical University, Wenzhou 325035, Zhejiang Province, China.; 2.Judical Forensic Center, Wenzhou Medical University, Wenzhou 325035, Zhejiang Province, China.; 3.Institute of Forensic Science, Wenzhou Medical University, Wenzhou 325035, Zhejiang Province, China.
第一作者单位	基础医学院（机能实验教学中心）_法医学系; 温州医科大学;
第一作者的第一单位	基础医学院（机能实验教学中心）_法医学系
推荐引用方式 GB/T 7714	J J Huang,Y Yao,C J Xia,et al. Relationship between the Number of Neutrophils and Myofibroblasts during Diabetic Wound Healing and Wound Age[J]. Fa yi xue za zhi,2019,35(2):149-153..
APA	J J Huang., Y Yao., C J Xia., Y D Zhao., S Yu., ... & Y Y Fan. (2019). Relationship between the Number of Neutrophils and Myofibroblasts during Diabetic Wound Healing and Wound Age. Fa yi xue za zhi, 35(2), 149-153..
MLA	J J Huang,et al."Relationship between the Number of Neutrophils and Myofibroblasts during Diabetic Wound Healing and Wound Age".Fa yi xue za zhi 35.2(2019):149-153..

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