[Effects of apple polyphenols on monocrotaline-induced pulmonary vascular remodeling in rats and its mechanism]

doi:10.12047/j.cjap.5769.2019.045

科研成果详情

题名	[Effects of apple polyphenols on monocrotaline-induced pulmonary vascular remodeling in rats and its mechanism]
其他题名	Effects of apple polyphenols on monocrotaline-induced pulmonary vascular remodeling in rats and its mechanism
作者	Shu-Hao Zhang 1; Si-Ming Shao2; Fang-Zheng Chen 1; Jing Zhu1; Luo-Wei Chen 1; Heng Wang 3; Xin-Hui Xiang 2; Lin-Bo Yuan4
发表日期	2019-05-28
发表期刊	Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology 影响因子和分区
语种	英语
原始文献类型	Article
关键词	苹果多酚肺动脉高压野百合碱(MCT) 大鼠细胞因子
摘要	Objective:To investigate the effects of apple polyphenols on pulmonary vascular remodeling in rats with pulmonary arterial hypertension and its mechanism. Methods:Rats were randomly divided into 4 groups:control (Con) group, monocrotaline (MCT) group, apple polyphenol (APP) group,monocrotaline + apple polyphenol (MCT+APP) group. In Con group, rats received a subcutaneous injection of physical saline. In APP group, rats received intraperitoneal injection of 20 mg/kg APP, every other day. In MCT group, rats received a single subcutaneous injection of MCT(60 mg/kg). In MCT+APP group, rats received subcutaneous injection of 60 mg/kg MCT followed by an intraperitoneal injection of 20 mg/kg APP every other day. All the disposal lasted 3 weeks. Then the PAH-relevant indicators, such as mean pulmonary artery pressure(mPAP), pulmonary vascular resistance(PVR), right ventricular hypertrophy index (RVHI) ,wall thickness (WT%) and wall area (WA%) were tested. After that, the inflammatory pathway related indicators, such as interleukin1(IL-1),interleukin1(IL-6), tumor necrosis factor α(TNF-α), cyclooxygenase 2(COX-2) and myeloperoxidase(MPO) in pulmonary tissue and free intracellular Ca2+ in pulmonary smooth muscle cell(PASMC), content of eNOS and NO in endothelial cells were determined. Results:Compared with the control group, the levels of mPAP, PVR, RVHI, WA%, WT%, and IL-1, IL-6, TNF-α, COX-2, MPO in tissue and the expression of Ca2 + in PASMC of MCT group were increased significantly, while the contents of eNOS and NO in endothelial cells were decreased significantly (P＜0.05). Compared with the MCT group, the apple polyphenol treatment could improve the above mentioned situation, and the COX-2 and Ca2+ indicators of the apple polyphenol treatment group were decreased significantly (P＜0.05). Conclusion:MCT can increase COX-2 expression and intracellular Ca2+ in pulmonary artery smooth muscle cells, decrease the contents of eNOS and NO in endothelial cells, while apple polyphenols can significantly inhibit these effects.
其他摘要	Objective: To investigate the effects of apple polyphenols on pulmonary vascular remodeling in rats with pulmonary arterial hypertension and its mechanism. Methods: Rats were randomly divided into 4 groups: control (Con) group,monocrotaline (MCT) group,apple polyphenol (APP) group,monocrotaline + apple polyphenol (MCT+APP) group. In Con group,rats received a subcutaneous injection of physical saline. In APP group,rats received intraperitoneal injection of 20 mg/kg APP,every other day. In MCT group,rats received a single subcutaneous injection of MCT(60 mg/kg). In MCT+APP group,rats received subcutaneous injection of 60 mg/kg MCT followed by an intraperitoneal injection of 20 mg/kg APP every other day. All the disposal lasted 3 weeks. Then the PAH-relevant indicators,such as mean pulmonary artery pressure(mPAP),pulmonary vascular resistance(PVR),right ventricular hypertrophy index (RVHI),wall thickness (WT%) and wall area (WA%) were tested. After that,the inflammatory pathway related indicators,such as interleukin1(IL-1), interleukin1(IL-6),tumor necrosis factor α(TNF-α),cyclooxygenase 2(COX-2) and myeloperoxidase(MPO) in pulmonary tissue and free intracellular Ca~(2+) in pulmonary smooth muscle cell(PASMC),content of eNOS and NO in endothelial cells were determined. Results: Compared with the control group,the levels of mPAP,PVR,RVHI,WA%, WT%,and IL-1,IL-6,TNF-α,COX-2,MPO in tissue and the expression of Ca~(2 +) in PASMC of MCT group were increased significantly, while the contents of eNOS and NO in endothelial cells were decreased significantly (P<0.05). Compared with the MCT group, the apple polyphenol treatment could improve the above mentioned situation,and the COX-2 and Ca~(2+) indicators of the apple polyphenol treatment group were decreased significantly (P<0.05). Conclusion: MCT can increase COX-2 expression and intracellular Ca~(2+) in pulmonary artery smooth muscle cells,decrease the contents of eNOS and NO in endothelial cells,while apple polyphenols can significantly inhibit these effects.
资助项目	地方高校国家级大学生创新创业训练计划 ; 浙江省温州市科技局项目 ; 浙江省新苗 ; 温州医科大学科研课题
出版者	NLM (Medline)
ISSN	1000-6834
卷号	35 期号:3 页码:209-214.
DOI	10.12047/j.cjap.5769.2019.045
页数	6
收录类别	PUBMED ; SCOPUS ; CSCD ; 万方 ; PKU ; 北大核心 ; ISTIC
学科领域	医药、卫生 ; 基础医学
URL	查看原文
CSCD记录号	CSCD:6529497
PubMed ID	31257800
SCOPUSEID	2-s2.0-85068969128
Scopus学科分类	Medicine (all)
万方分类号	R364.3+1(病理学)
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/33986
专题	第一临床医学院（信息与工程学院）、附属第一医院眼视光学院（生物医学工程学院）、附属眼视光医院仁济学院基础医学院（机能实验教学中心）_机能学系_生理学
作者单位	1.School of the First Clinical Medical Sciences, Wenzhou Medical University.; 2.Ren ji College of Wenzhou Medical University.; 3.College of Optometry, Wenzhou Medical University.; 4.Department of Physiology, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou 325000, China.
第一作者单位	第一临床医学院（信息与工程学院）、附属第一医院
第一作者的第一单位	第一临床医学院（信息与工程学院）、附属第一医院
推荐引用方式 GB/T 7714	Shu-Hao Zhang,Si-Ming Shao,Fang-Zheng Chen,et al. [Effects of apple polyphenols on monocrotaline-induced pulmonary vascular remodeling in rats and its mechanism][J]. Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology,2019,35(3):209-214..
APA	Shu-Hao Zhang., Si-Ming Shao., Fang-Zheng Chen., Jing Zhu., Luo-Wei Chen., ... & Lin-Bo Yuan. (2019). [Effects of apple polyphenols on monocrotaline-induced pulmonary vascular remodeling in rats and its mechanism]. Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology, 35(3), 209-214..
MLA	Shu-Hao Zhang,et al."[Effects of apple polyphenols on monocrotaline-induced pulmonary vascular remodeling in rats and its mechanism]".Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology 35.3(2019):209-214..

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