[Effects of AdipoRon orally on the functions of spleen and pancreas in type 2 diabetic mice]

doi:10.12047/j.cjap.5589.2019.015

科研成果详情

题名	[Effects of AdipoRon orally on the functions of spleen and pancreas in type 2 diabetic mice]
其他题名	Effects of AdipoRon orally on the functions of spleen and pancreas in type 2 diabetic mice
作者	Ke-Jian Xie; Ling Huang; Xiao-Hu Qu; Xue Li; Shao-Jie Wang; Min Xiao
发表日期	2019
发表期刊	Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology 影响因子和分区
语种	英语
原始文献类型	Article
关键词	AdipoRon 2型糖尿病胰腺脾脏小鼠
摘要	Objective:To observe the effects of AdipoRon orally on the functions of spleen and pancreas in type 2 diabetic mice, in order to present data for clinical application. Methods:Forty C57/BL6 male mice were randomly divided into 2 groups: normal control group (n=10) and model group (n=30), the former group was fed normally, while the later group was fed with high fat and sugar for 4 weeks.After that, type 2 diabetes model was established in DM group induced by intraperitoneal injection of streptozotocin (STZ, 40 mg/kg).As type 2 diabetes model established successfully, the model mice were randomly divided into three groups (n=10): diabetes mellitus (DM) group, high dose of AdipoRon group (DM + H) and low dose of adiponRon group (DM + L).All the four groups were treated with saline, saline, AdipoRon at the doses of 20 mg/kg and 50 mg/kg by gavages respectively, once a day for 10 days.And then put them to death for collecting blood, pancreas and spleen.Pathological changes of pancreas were observed with a light microscope after HE staining.Protein contents of insulin receptor (INSR), insulin receptor substrate 1( IRS-1) and tumor necrosis factor-α(TNF-α) in pancreatic and spleen tissues were detected by ELISA.The protein level of phosphorylation insulin receptor substrate 1(p-IRS-1) in pancreas was determined by Western blot, and the expression of insulin mRNA in pancreas was tested by RT-PCR. Results:Under the light microscope, it was visible that the pancreatic tissue in NC group was full and closely packed, and the islet was big.Pancreatic tissue of DM mice was incompact and the islet of DM mice was smaller than that of normal mice.As for the mice treated with AdipoRon orally, the pancreatic tissue was full and closely arranged, and the islet was slightly smaller.Compared with NC group, the levels of TNF-α in pancreas and spleen of DM group were increased markedly, the levels of INSR and IRS-1 were decreased, the spleen coefficient, p-IR-1 protein level and insulin mRNA expression in pancreas were decreased, all were significant statistically (P＜0.05).Compared with DM group, the levels of TNF-α in pancreas and spleen of AdipoRon groups were decreased, the levels of INSR and IRS-1 in pancreas and spleen of AdipoRon groups were increased, while the spleen coefficient was increased (P＜0.05).The p-IRS-1 protein level and insulin mRNA expression in pancreas in DM+H group were increased (P＜0.05).Compared with DM + L group, the level of TNF-α was decreased, and the levels of INSR and IRS-1 were significantly increased (P＜0.05) in DM + H group (P＜0.05). Conclusion:Oral administration of AdipoRon can protect the spleen and pancreas of diabetic mice by decreasing the inflammatory response, up-regulating the expression of INSR, and increasing p-IRS-1 level in diabetic mice.
资助项目	2015年度浙江省公益技术应用研究计划(实验动物);
ISSN	1000-6834
卷号	35 期号:1 页码:60-64.
DOI	10.12047/j.cjap.5589.2019.015
页数	5
收录类别	PUBMED ; SCOPUS ; 万方 ; PKU ; 北大核心 ; ISTIC
URL	查看原文
Pubmed记录号	31245954
Scopus记录号	2-s2.0-85068911478
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/33976
专题	基础医学院（机能实验教学中心）_生物科学系
作者单位	Department of Biology, School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou 325035 China.
第一作者单位	检验医学院（生命科学学院、生物学实验教学中心）
第一作者的第一单位	检验医学院（生命科学学院、生物学实验教学中心）
推荐引用方式 GB/T 7714	Ke-Jian Xie,Ling Huang,Xiao-Hu Qu,et al. [Effects of AdipoRon orally on the functions of spleen and pancreas in type 2 diabetic mice][J]. Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology,2019,35(1):60-64..
APA	Ke-Jian Xie, Ling Huang, Xiao-Hu Qu, Xue Li, Shao-Jie Wang, & Min Xiao. (2019). [Effects of AdipoRon orally on the functions of spleen and pancreas in type 2 diabetic mice]. Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology, 35(1), 60-64..
MLA	Ke-Jian Xie,et al."[Effects of AdipoRon orally on the functions of spleen and pancreas in type 2 diabetic mice]".Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology 35.1(2019):60-64..

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