[The effect of AdipoRon on insulin sensitivity of mouse skeletal muscle cells and its mechanism]

doi:10.12047/j.cjap.5533.2017.078

科研成果详情

题名	[The effect of AdipoRon on insulin sensitivity of mouse skeletal muscle cells and its mechanism]
其他题名	The effect of AdipoRon on insulin sensitivity of mouse skeletal muscle cells and its mechanism
作者	Min Xiao; Xiao-Hu Qu; Hui Chen; Li-Qin Jin
发表日期	2017-04-08
发表期刊	Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology 影响因子和分区
语种	英语
原始文献类型	Article
关键词	AdipoRon PI3K insulin resistance mouse myoblast cell line (C2C12).
其他关键词	insulin resistance ; AdipoRon ; PI3K ; mouse myoblast cell line (C2C12)
摘要	Objective:To observe the effect of AdipoRon, an adiponin receptor agonist, on insulin sensitivity in mouse myoblast cell line (C2C12) and to explore its mechanism. Methods:C2C12 was induced to differentiate into myoblasts by using horse serum. Then the cells were divided into 6 groups (9 double wells):blank control group, high dose AdipoRon group, low dose AdipoRon group, insulin group and the low dose AdipoRon with PI3K inhibitor (phosphatidylinositol 3 kinase) group and the insulin with PI3K inhibitor group. After cultured for 12 h, the supernatant was collected and glucose consumption was measured. Cell proliferation was tested by using CCK8. In the 6-well plate, C2C12 was induced to differentiate into myoblasts. The drug was incubated for 12 h and the mRNA level of GLUT4 was detected by RT-PCR. Results:Compared with the blank control group, the levels of glucose consumption in high dose AdipoRon group, low dose AdipoRon group and insulin group was increased significantly (P<0.05). After adding PI3K inhibitor, the levels of glucose consumption in the above mentioned three groups were not different from that in blank control group. high dose AdipoRon group, low dose AdipoRon group and insulin group had proliferation, but only the insulin group was statistically significant (P<0.05). Compared with the control group, the levels of GLUT4 mRNA in AdipoRon high dose group, low dose AdipoRon group and insulin group were all higher than those in control group (P<0.05). After adding PI3K inhibitor, GLUT4 mRNA level was not statistically significant compared with blank control group. Conclusions:AdipoRon can increase the consumption of glucose without affecting cell proliferation, which may play a role in improving insulin sensitivity, but the specific mechanism remains to be further studied.
其他摘要	Objective: To observe the effect of AdipoRon, an adiponin receptor agonist, on insulin sensitivity in mouse myoblast cell line(C2C12) and to explore its mechanism. Methods: C2C12 was induced to differentiate into myoblasts by using horse serum. Then the cells were divided into 6 groups (9 double wells) : blank control group, high dose AdipoRon group, low dose AdipoRon group, insulin group and the low dose AdipoRon with PI3K inhibitor (phosphatidylinositol 3 kinase) group and the insulin with PI3K inhibitor group. After cultured for 12 h, the supernatant was collected and glucose consumption was measured. Cell proliferation was tested by using CCK8. In the 6-well plate, C2C12 was induced to differentiate into myoblasts. The drug was incubated for 12 h and the mRNA level of GLUT4 was detected by RT-PCR. Results: Compared with the blank control group, the levels of glucose consumption in high dose AdipoRon group, low dose AdipoRon group and insulin group was increased significantly (P < 0.05). After adding PI3K inhibitor, the levels of glucose consumption in the above mentioned three groups were not different from that in blank control group, high dose AdipoRon group, low dose AdipoRon group and insulin group had proliferation, but only the insulin group was statistically significant ( P < 0.05). Compared with the control group, the levels of GLUT4 mRNA in AdipoRon high dose group, low dose AdipoRon group and insulin group were all higher than those in control group (P < 0.05) . After adding PBK inhibitor, GLUT4 mRNA level was not statistically significant compared with blank control group. Conclusion: AdipoRon can increase the consumption of glucose without affecting cell proliferation, which may play a role in improving insulin sensitivity, but the specific mechanism remains to be further studied.
资助项目	2015年度浙江省公益技术应用研究计划(实验动物)
ISSN	1000-6834
卷号	33 期号:4 页码:319-322.
DOI	10.12047/j.cjap.5533.2017.078
页数	4
收录类别	PUBMED ; SCOPUS ; CSCD ; 万方 ; PKU ; 北大核心 ; ISTIC
学科领域	医药、卫生 ; 内科学
URL	查看原文
CSCD记录号	CSCD:6084179
PubMed ID	29926636
SCOPUSEID	2-s2.0-85056635604
Scopus学科分类	Medicine (all)
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/33972
专题	基础医学院（机能实验教学中心）_生物科学系
作者单位	Department of Biology, School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou 325035, China.
第一作者单位	检验医学院（生命科学学院、生物学实验教学中心）
第一作者的第一单位	检验医学院（生命科学学院、生物学实验教学中心）
推荐引用方式 GB/T 7714	Min Xiao,Xiao-Hu Qu,Hui Chen,et al. [The effect of AdipoRon on insulin sensitivity of mouse skeletal muscle cells and its mechanism][J]. Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology,2017,33(4):319-322..
APA	Min Xiao, Xiao-Hu Qu, Hui Chen, & Li-Qin Jin. (2017). [The effect of AdipoRon on insulin sensitivity of mouse skeletal muscle cells and its mechanism]. Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology, 33(4), 319-322..
MLA	Min Xiao,et al."[The effect of AdipoRon on insulin sensitivity of mouse skeletal muscle cells and its mechanism]".Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology 33.4(2017):319-322..

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