[Effect of dexmedetomidine on renal injury induced by lung ischemia/reperfusion in mice]

doi:10.12047/j.cjap.5500.2017.092

科研成果详情

题名	[Effect of dexmedetomidine on renal injury induced by lung ischemia/reperfusion in mice]
其他题名	Effect of dexmedetomidine on renal injury induced by lung ischemia/reperfusion in mice
作者	Bing-Qian Xiang 1; Hui Gao1; Zi-Yin Luo 1; Zhou-Xi Fang2; Wan-Tie Wang1
发表日期	2017-04-08
发表期刊	Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology 影响因子和分区
语种	英语
原始文献类型	Article
关键词	IL-1β TNF-α dexmedetomidine ischemia/reperfusion injury renal.
其他关键词	dexmedetomidine ; ischemia/reperfusion injury ; renal ; IL-1β ; TNF-α
摘要	Objective:To evaluate the effect of dexmedetomidine(Dex) on renal injury induced by lung ischemia/reperfusion(I/R) in mice. Methods:Fifty healthy SPF male C57BL/6J mice, weighing 20 g~24 g,aged 8~10 weeks,were randomly divided into five groups(n=10 each):sham operation group(sham group),lung ischemia/reperfusion group(I/R group), lung ischemia/reperfusion and normal saline group (NS group), dexmedetomidine group(Dex group), dexmedetomidine and atipamezole group (DA group). Lung ischemia/reperfusion model was established by occlusion of the left pulmonary artery for 30 min followed by 180 min reperfusion in mice. In Dex and DA groups, dexmedetomidine 20 μg/kg and dexmedetomidine 20 μg/kg plus atipamezole 250 μg/kg were injected intraperitoneally respectively at 30 min before establishment of the model, isopyknic normal saline instead of Dex were injected intraperitoneally in NS group. After the experiment the mice were killed and plasma IL-1 beta and tumor necrosis factor α(TNF-α) concentration were detected by ELISA; the renal tissues were harvested to observe ultra structure under electron microscope. Results:Compared with sham group, the concentrations of IL-1β and TNF-α in other groups were increased significantly and the structure damages of renal tissues observed under electron microscope in other groups were more serious than those of sham group. Compared with I/R group, NS groups and DA group, the concentrations of IL-1β and TNF-α in Dex group were significantly lower(P<0.05)and the structure damages of renal tissues observed under electron microscope in Dex group were slighter. Conclusions:Dexmedetomidine pretreatment can attenuate renal injury induced by lung ischemia/reperfusion and the mechanism may be related to inhibition of inflammatory responses.
其他摘要	Objective: To evaluate the effect of dexmedetomidine(Dex) on renal injury induced by lung ischemia/reperfusion(I/R) in mice. Methods: Fifty healthy SPF male C57BL/6J mice, weighing 20 g ~24 g,aged 8 ~ 10 weeks, were randomly divided into five groups( n =10 each) : sham operation group(sham group), lung ischemia/reperfusion group(I/R group),lung ischemia/reperfusion and normal saline group (NS group),dexmedetomidine group(Dex group) , dexmedetomidine and atipamezole group (DA group). Lung ischemia / reperfusion model was established by occlusion of the left pulmonary artery for 30 min followed by 180 min reperfusion in mice. In Dex and DA groups, dexmedetomidine 20 μg/kg and dexmedetomidine 20 μg/kg plus atipamezole 250 μg/kg were injected intraperitoneally respectively at 30 min before establishment of the model,isopyknic normal saline instead of Dex were injected intraperitoneally in NS group. After the experiment the mice were killed and plasma IL-1 beta and tumor necrosis factor a(TNF-a) concentration were detected by ELISA; the renal tissues were harvested to observe ultra structure under electron microscope. Results: Compared with sham group, the concentrations of IL-1β and TNF-a in other groups were increased significantly and the structure damages of renal tissues observed under electron microscope in other groups were more serious than those of sham group. Compared with I/R group, NS groups and DA group, the concentrations of IL-1β and TNF-a in Dex group were significantly lower( P < 0.05) and the structure damages of renal tissues observed under electron microscope in Dex group were slighter. Conclusion: Dexmedetomidine pretreatment can attenuate renal injury induced by lung ischemia/reperfusion and the mechanism may be related to inhibition of inflammatory responses.
资助项目	浙江省公益技术应用研究项目 ; 浙江省新苗人才计划项目 ; 温州市公益性科技计划项目
ISSN	1000-6834
卷号	33 期号:4 页码:380-384.
DOI	10.12047/j.cjap.5500.2017.092
页数	5
收录类别	PUBMED ; SCOPUS ; CSCD ; 万方 ; PKU ; 北大核心 ; ISTIC
学科领域	医药、卫生 ; 基础医学
URL	查看原文
CSCD记录号	CSCD:6084193
PubMed ID	29926648
SCOPUSEID	2-s2.0-85056665763
Scopus学科分类	Medicine (all)
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/33969
专题	温州医科大学检验医学院（生命科学学院、生物学实验教学中心）_生物学实验教学中心_显微镜室、电镜室
作者单位	1.Ischemia/Reperfusion Injury Research Institute, Wenzhou Medical University, Wenzhou 325035, China.; 2.Department of Electron Microscopy, Wenzhou Medical University, Wenzhou 325035, China.
第一作者单位	温州医科大学
第一作者的第一单位	温州医科大学
推荐引用方式 GB/T 7714	Bing-Qian Xiang,Hui Gao,Zi-Yin Luo,et al. [Effect of dexmedetomidine on renal injury induced by lung ischemia/reperfusion in mice][J]. Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology,2017,33(4):380-384..
APA	Bing-Qian Xiang, Hui Gao, Zi-Yin Luo, Zhou-Xi Fang, & Wan-Tie Wang. (2017). [Effect of dexmedetomidine on renal injury induced by lung ischemia/reperfusion in mice]. Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology, 33(4), 380-384..
MLA	Bing-Qian Xiang,et al."[Effect of dexmedetomidine on renal injury induced by lung ischemia/reperfusion in mice]".Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology 33.4(2017):380-384..

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