[Protective effects of Curcumin analogue L6H4 on kidney from type 2 diabetic rats]

doi:10.12047/j.cjap.5448.2017.003

科研成果详情

题名	[Protective effects of Curcumin analogue L6H4 on kidney from type 2 diabetic rats]
其他题名	Protective effects of Curcumin analogue L6H4 on kidney from type 2 diabetic rats
作者	Xi Liu 1; Jun Ma2; Mao-Tong Hu 2; Xi-Dan Dong2; Lan-Ting Xiang 2; Qian-Ru Gu 2; Zhuan-Yun Du 3; San-Mei Chen 4; Guo-Rong Chen2
发表日期	2017-01-08
发表期刊	Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology 影响因子和分区
语种	英语
原始文献类型	Article
关键词	curcumin analogue L6H4 kidney rat transforming growth factor-beta 1 type 2 diabetes.
摘要	Objective:To investigate the protective effect of curcumin analogue L6H4 on the kidney from the type 2 diabetic rats. Methods:Twenty-four SPF male SD rats were randomly divided into 3 groups(n=8):normal control group(NC),diabetes mellitus group(DM) and DM+L6H4-treatment group(DT). After rats were fed with high-fat diet for 4 weeks, both the DM and DT groups were injected with streptozotocin intraperitoneally to induce type 2 diabetes mellitus models. The rats in DT group were given L6H4 by gavage at the dose of 0.2 mg/kg·d for 8 weeks. After the treatment, the 24 h urinary protein, fasting blood glucose (FBG), triglyceride (TG), serum creatinine(Scr),blood urea nitrogen (BUN) and uric acid (UA) were detected biochemically. The pathological changes of the kidneys were observed under light and transmission electron microscopes. The expressions of TGF-β1, FN and Col IV were detected by immunohistochemistry. Results:The levels of the 24 h urinary protein, FBG, TG, Scr and BUN were elevated significantly in diabetic group(P<0.01). The glomerular volume of DM group rats became irregularly enlarged, diffused mesangial matrix accumulated, with basal membrane proliferous hypertrophy and fusion phenomenon of foot process, the expressions of TGF-β1,FN and Col-IV were elevated significantly (P<0.05). After treated with L6H4, the levels of the 24 h urinary protein, FBG, TG, Scr and BUN were decreased in DT group compared to DM group (P<0.01), the morphological changes of kidney were ameliorated. The expression levels of TGF-β1, FN and Col-IV were downregulated (P<0.05). Conclusions:L6H4 exerts the protective effect on kidneys of type 2 diabetic rats by reducing expression of TGF-β1, inhibiting secretion of Col-IV and FN, relieving the deposition of the extracellular matrix.
其他摘要	Objective:To investigate the protective effect of curcumin analogue L6H4 on the kidney from the type 2 diabetic rats. Methods:Twenty-four SPF male SD rats were randomly divided into 3 groups(n = 8):normal control group(NC),diabetes mellitus group(DM) and DM + L6H4-treatment group(DT). After rats were fed with high-fat diet for 4 weeks,both the DM and DT groups were injected with streptozotocin intraperitoneally to induce type 2 diabetes mellitus models. The rats in DT group were given L6H4 by gavage at the dose of 0.2 mg /kg·d for 8 weeks. After the treatment,the 24 h urinary protein,fasting blood glucose (FBG),triglyceride(TG),serum creatinine(Scr),blood urea nitrogen (BUN) and uric acid (UA) were detected biochemically. The pathological changes of the kidneys were observed under light and transmission electron microscopes. The expressions of TGF-β1,FN and Col IV were detected by immunohistochemistry. Results:The levels of the 24 h urinary protein,FBG,TG,Scr and BUN were elevated significantly in diabetic group(P < 0.01). The glomerular volume of DM group rats became irregularly enlarged,diffused mesangial matrix accumulated,with basal membrane proliferous hypertrophy and fusion phenomenon of foot process,the expressions of TGF-β1,FN and Col-IV were elevated significantly (P < 0.05). After treated with L6H4,the levels of the 24 h urinary protein,FBG,TG,Scr and BUN were decreased in DT group compared to DM group (P < 0.01),the morphological changes of kidney were ameliorated. The expression levels of TGF-β1,FN and Col-IV were downregulated (P < 0.05). Conclusion:L6H4 exerts the protective effect on kidneys of type 2 diabetic rats by reducing expression of TGF-β1,inhibiting secretion of Col-IV and FN,relieving the deposition of the extracellular matrix.
资助项目	浙江省公益性技术应用研究计划项目
ISSN	1000-6834
卷号	33 期号:1 页码:11-15.
DOI	10.12047/j.cjap.5448.2017.003
收录类别	PUBMED ; SCOPUS ; CSCD
学科领域	医药、卫生 ; 基础医学
URL	查看原文
CSCD记录号	CSCD:5903342
PubMed ID	29926600
SCOPUSEID	2-s2.0-85055614162
Scopus学科分类	Medicine (all)
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/33964
专题	附属第一医院基础医学院（机能实验教学中心）_生物科学系
作者单位	1.Department of Nephrology, the Affiliated Wenzhou Chinese Medical Hospital of Zhejiang Chinese Medical University, Wenzhou 325000.; 2.Department of Pathology, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325015.; 3.Department of Biology, School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou 325035.; 4.Medical College, Shaoxing University, Shaoxing 312000, China.
推荐引用方式 GB/T 7714	Xi Liu,Jun Ma,Mao-Tong Hu,et al. [Protective effects of Curcumin analogue L6H4 on kidney from type 2 diabetic rats][J]. Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology,2017,33(1):11-15..
APA	Xi Liu., Jun Ma., Mao-Tong Hu., Xi-Dan Dong., Lan-Ting Xiang., ... & Guo-Rong Chen. (2017). [Protective effects of Curcumin analogue L6H4 on kidney from type 2 diabetic rats]. Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology, 33(1), 11-15..
MLA	Xi Liu,et al."[Protective effects of Curcumin analogue L6H4 on kidney from type 2 diabetic rats]".Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology 33.1(2017):11-15..

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